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3.2.1.67: galacturonan 1,4-alpha-galacturonidase

This is an abbreviated version!
For detailed information about galacturonan 1,4-alpha-galacturonidase, go to the full flat file.

Word Map on EC 3.2.1.67

Reaction

[(1->4)-alpha-D-galacturonide]n
+
H2O
=
[(1->4)-alpha-D-galacturonide]n-1
+
D-galacturonate

Synonyms

alkaline exo-polygalacturonase, carrot exopolygalacturonase, CbPelA, corn pollen polygalacturonase, EPG, exo polygalacturonase, exo-D-galacturonanase, exo-D-galacturonase, Exo-PG, exo-PGase, exo-PGI, exo-PGII, exo-PGU B, exo-poly alpha galactouronosidase, exo-polygalacturonanase, exo-polygalacturonase, exolytic PGase, exoPG, exoPG1, exoPG2, exoPGase, exopoly-D-galacturonase, exopolygalacturonanase, exopolygalacturonase, exopolygalacturonase B, exopolygalacturonase X, Galacturan 1,4-alpha-galacturonidase, GH28, macerating enzyme, More, MpPG1, MpPG3, oligogalacturonate hydrolase, peach exopolygalacturonase, PECI, pectinase, PelB, PG III, PGase, PGase I, PGC2, PGC3, PGI, poly 1,4-alpha-D-galacturonide-galacturonohydrolase, Poly(1,4-alpha-D-galacturonide)galacturonohydrolase, poly(galacturonate)hydrolase, poly-(1-4)-alpha-D-galactosiduronate glycanohydrolase, polygalacturonanase, polygalacturonase, polymethylgalacturonase, RPG15, RPG16, SSXPG1, SSXPG2, TM0437, TmGalU, YeGH2

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.67 galacturonan 1,4-alpha-galacturonidase

Cloned

Cloned on EC 3.2.1.67 - galacturonan 1,4-alpha-galacturonidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) CodonPlus cells
Thermotoga thermophilus
-
expressed in Escherichia coli Rosetta cells
-
expressed in Pichia pastoris strain SMD1168
expressed in Pichia pastoris strains of SMD1168
expression in Escherichia coli
gene ACM61449, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain origami(DE3)
-
gene pehY, pYEII-4-H192, cloned and expressed in Escherichia coli
Yersinia enterolytica
-
gene pgaX cloned by reverse genetics, pIM361, overexpression of the enzyme by transformants
-
gene pgu B, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21DE3
isozyme MpPG1, DNA and amino acid sequence determination and analysis
isozyme MpPG3, DNA and amino acid sequence determination and analysis
recombinant expression of the wild-type full-length enzyme and its catalytic domain of polygalacturonase in Pichia pastoris strain GS115
-
the YeGH28 construct is then transformed into BL21 DE3 star cells
-