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2.5.1.21: squalene synthase

This is an abbreviated version!
For detailed information about squalene synthase, go to the full flat file.

Word Map on EC 2.5.1.21

Reaction

2 (2E,6E)-farnesyl diphosphate +

NAD(P)H
+
H+
=
squalene
 + 2 diphosphate +
NAD(P)+

Synonyms

BbSS, BSS, CrSQS, dt-ySQase, Erg9, EtSS, farnesyl-diphosphate farnesyltransferase, farnesyl-diphosphate:farnesyldiphosphate farnesyltransferase, farnesyldiphosphate farnesyltransferase 1, farnesyldiphosphate:farnesyldiphosphate farnesyltransferase, farnesyltransferase, FDFT1, hSQS, presqualene synthase, presqualene-diphosphate synthase, SgSQS, SQase, SQS, SQS1, SQS2, squalene synthase, squalene synthase 1, squalene synthase 2, squalene synthetase, SS1, SSase, SSN, synthase, squalene, TkSQS1, TkSQS2

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.21 squalene synthase

Purification

Purification on EC 2.5.1.21 - squalene synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in E. coli
-
gel purification and Ni-NTA column purification
-
HiTrap nickel-chelating column chromatography and MonoQ HR5/5 anion-exchange column chromatography
-
overexpressed in E. coli
-
partial
partially purified recombinant enzyme in microsomes is further purified by ultracentrifugation
recombinant C-terminally His6-tagged and truncated enzyme from Escherichia coli by cobalt affinity chromatography
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol
Thermosynechococcus vestitus
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, the N-terminal His-tag is then cleaved using thrombin and removed using nickel affinity chromatography
recombinant His6-tagged enzyme from Pichia pastoris strain GS115 by metal affinity chromatography to 97% purity
recombinant His6-tagged LdSSN from Escherichia coli strain BL21(DE3) by anion exchange chromatography, nickel affinity chrmatography is not successful
recombinant His6-tagged WsSQS1and WsSQS2 proteins from Escherichia coli by nickel affinity chromatgraphy
recombinant protein
-
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography, supplementation of any type of detergent inhibits the enzyme activity
recombinant soluble His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant truncated N-terminally His6-tagged SQS(31-370) from Escherichia coli strain BL21(DE3), the tag is cleaved off by thrombin
-
trypsin-truncated enzyme
-