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Literature summary for 2.5.1.21 extracted from

  • Kuswik-Rabiega, G.; Rilling, H.C.
    Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities (1987), J. Biol. Chem., 262, 1505-1509.
    View publication on PubMed
Search for more literature for 2.5.1.21

General Stability

General Stability Organism
methanol stabilizes Saccharomyces cerevisiae
sucrose stabilizes Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for squalene synthase reaction Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.95
-
 squalene Saccharomyces cerevisiae
15.6
-
 presqualene Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + farnesyl diphosphate
-
 Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
 ?
presqualene diphosphate + NAD(P)H presqualene diphosphate synthetase and squalene synthetase are copurified during isolation Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Saccharomyces cerevisiae
NADPH preference for NADPH over NADH Saccharomyces cerevisiae