Cloned (Comment) | Organism |
---|---|
gene ERG9, recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 | Candida tropicalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | heterologous expression of antigenic enzyme from Candida tropicalis in Pichia pastoris can be exploited for large-scale production | Candida tropicalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Candida tropicalis | 16020 | - |
microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
- |
Candida tropicalis |
57000 | - |
- |
Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Candida tropicalis | - |
squalene + 2 diphosphate + NADP+ | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Candida tropicalis ATCC MYA-3404 | - |
squalene + 2 diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | C5M7M6 | gene ERG9 | - |
Candida tropicalis ATCC MYA-3404 | C5M7M6 | gene ERG9 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Pichia pastoris strain GS115 by metal affinity chromatography to 97% purity | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Candida tropicalis | squalene + 2 diphosphate + NADP+ | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Candida tropicalis ATCC MYA-3404 | squalene + 2 diphosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, recombinant His6-tagged enzyme, SDS-PAGE, x * 55000, about, sequence calculation of His6-tagged enzyme | Candida tropicalis |
Synonyms | Comment | Organism |
---|---|---|
SQS | - |
Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Candida tropicalis |