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Literature summary for 2.5.1.21 extracted from

  • Zhang, D.; Jennnings, S.M.; Robinson, G.W.; Poulter, C.D.
    Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme [published erratum appears in Arch Biochem Biophys 1993 Sep;305(2):622] (1993), Arch. Biochem. Biophys., 304, 133-143.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activates recombinant enzyme Saccharomyces cerevisiae
Tween 20 stimulates recombinant enzyme Saccharomyces cerevisiae
Tween 40 stimulates recombinant enzyme Saccharomyces cerevisiae
Tween 80 stimulates recombinant enzyme Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
development of a plasmid for heterologous expression of yeast squalene synthase in Escherichia coli Saccharomyces cerevisiae

General Stability

General Stability Organism
methanol, 10%, does not stabilize Saccharomyces cerevisiae
octylglucoside, 5 mM, causes rapid loss of activity Saccharomyces cerevisiae
stable in buffer containing 5 mM octylglucoside and 15 mM CHAPS Saccharomyces cerevisiae
sucrose, 10%, does not stabilize Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for squalene synthase reaction Saccharomyces cerevisiae
Mg2+ Mn2+ is more effective than Mg2+ in activation of dehydrosqualene formation Saccharomyces cerevisiae
Mn2+ stimulates, maximum stimulation at 0.1 mM Saccharomyces cerevisiae
Mn2+ more effective than Mg2+ in activation of dehydrosqualene formation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + farnesyl diphosphate Saccharomyces cerevisiae first pathway-specific enzyme in cholesterol biosynthesis diphosphate + presqualene diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
expressed in E. coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-70°C, stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + farnesyl diphosphate
-
Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
farnesyl diphosphate + farnesyl diphosphate first pathway-specific enzyme in cholesterol biosynthesis Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
presqualene diphosphate + NAD(P)H
-
Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 44000, expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
farnesyl diphosphate in presence of 1% Tween 80 and Mg2+ Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.2 pH 5.2: about 45% of maximum activity, pH 8.2: about 75% of maximum activity Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
irreversible denaturation above Saccharomyces cerevisiae