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Literature summary for 2.5.1.21 extracted from

  • Huang, D.; Yao, Y.; Zhang, H.; Mei, Z.; Wang, R.; Feng, L.; Liu, B.
    Directed optimization of a newly identified squalene synthase from Mortierella alpine based on sequence truncation and site-directed mutagenesis (2015), J. Ind. Microbiol. Biotechnol., 42, 1341-1352.
    View publication on PubMed
Search for more literature for 2.5.1.21

Application

Application Comment Organism
synthesis the engineered enzym emutant mMaSQS?C17 (E186K) is a potential candidate of the terpenes and steroids synthesis employed for synthetic biology Mortierella alpina

Cloned(Commentary)

Cloned (Comment) Organism
gene SQS, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Mortierella alpina

Protein Variants

Protein Variants Comment Organism
E186K site-directed mutagenesis, the engineered enzyme E186K mutant mMaSQSDELTAC17 shows a 3.4fold improvement in catalytic efficiency (kcat/Km) compared to the control Mortierella alpina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetic model Mortierella alpina
0.0052
-
 (2E,6E)-farnesyl diphosphate pH 7.2, 37°C, recombinant mutant enzyme Mortierella alpina
0.242
-
 NADPH pH 7.2, 37°C, recombinant mutant E186K enzyme Mortierella alpina

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mortierella alpina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, recombinant enzyme, SDS-PAGE Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Mortierella alpina
-
 squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Mortierella alpina ATCC 32222
-
 squalene + 2 diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alpina A0A0K2F2K4 gene SQS
-
Mortierella alpina ATCC 32222 A0A0K2F2K4 gene SQS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Mortierella alpina squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Mortierella alpina ATCC 32222 squalene + 2 diphosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, recombinant enzyme, SDS-PAGE Mortierella alpina

Synonyms

Synonyms Comment Organism
SQS
-
 Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mortierella alpina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 activity range, profile overview Mortierella alpina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
 (2E,6E)-farnesyl diphosphate pH 7.2, 37°C, recombinant mutant E186K enzyme Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Mortierella alpina

pH Range

pH Minimum pH Maximum Comment Organism
6.9 9 activity range, profile overview Mortierella alpina

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Mortierella alpina

General Information

General Information Comment Organism
evolution the enzyme belongs to the isoprenoid biosynthesis enzymes class 1 superfamily Mortierella alpina
metabolism the enzyme is a key enzyme in the isoprenoid biosynthesis Mortierella alpina
additional information structure homology modelling using the crystal structure of human squalene synthase, PDB ID 1EZFB, as template, overview Mortierella alpina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
90
-
 (2E,6E)-farnesyl diphosphate pH 7.2, 37°C, recombinant mutant E186K enzyme Mortierella alpina