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1.4.1.14: glutamate synthase (NADH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADH), go to the full flat file.

Wordmap for 1.4.1.14

Word Map on EC 1.4.1.14 Wordmap for 1.4.1.14

Reaction

2 L-glutamate +

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NAD+
=
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L-glutamine
+
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2-oxoglutarate
+
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NADH
+
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H+

Synonyms

gltA, GltA2, gltB, glutamate (reduced nicotinamide adenine dinucleotide) synthase, glutamate synthase, glutamate synthase (NADH), glutamate synthase (NADH-dependent), glutamate synthetase, glutamine 2-oxoglutarate aminotransferase, GOGAT, GOGAT1, GOGAT2, L-glutamate synthase (NADH), L-glutamate synthetase, NADH glutamate synthase, NADH-dependent glutamate synthase, NADH-dependent glutamine-2-oxoglutarate aminotransferase, NADH-GltS, NADH-glutamate synthase, NADH-GOGAT, NADH-GOGAT I, NADH-GOGAT II, NADH-GOGAT1, NADH-GOGAT2, NADH: GOGAT, Os01g0681900, Os05g0555600, synthase, glutamate (reduced nicotinamide adenine dinucleotide)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                EC 1.4.1.141.4.1.14 glutamate synthase (NADH)

Inhibitors

Inhibitors on EC 1.4.1.14 - glutamate synthase (NADH)

for references in articles please use BRENDA:EC1.4.1.14

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,5-dioxoimidazolidin-4-yl)urea
-
activity of NADH-GOGAT I significantly decreases in the presence of 10 mM allantoine
(2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid
2-hydroxyglutarate
-
D- and L-isomer, most potent competitive inhibitor
2-oxoglutarate
-
-
3-acetylpyridine adenine dinucleotide
-
competitive inhibitor with respect to NADH
6-diazo-5-oxo-L-norleucine
acridine
-
inhibitor of flavoenzymes, 65% inhibition
allopurinol
-
activity of NADH-GOGAT I significantly decreases in the presence of 2 mM allopurinol
arginine
-
20 mM, complete inhibition
asparagine
aspartate
azaserine
bathophenanthroline
-
50% inhibition
Cd2+
-
0.5 mM slightly decreases GOGAT activity in the absence of 2-oxoglutarate
citrate
-
inhibited by high concentrations, 40 mM
Co2+
-
slight inhibition
Cu2+
-
Cu2+ and Fe2+ are most inhibitory metal ions
D-2-hydroxyglutarate
-
-
D-glutamate
-
competitive inhibitor
D-glutamine
DL-ethionine sulfone
DL-homocysteic acid
-
-
Fe2+
-
Cu2+ and Fe2+ are most inhibitory metal ions
glutamine
-
activity of NADH-GOGAT I significantly decreases in the presence of 10 mM glutamine
Glutarate
-
most potent competitive inhibitor
glycine
L-2-Amino-4-oxo-5-chloropentanoate
-
1 mM, 30 min, 0┬░C, pH 7.6, irreversible inhibition
L-2-hydroxyglutarate
-
-
L-Albizziin
L-glutamate
L-histidine
-
25 mM, 20-30% inhibition
L-homocysteine sulfonamide
-
very potent transition state inhibitor, competitive to L-glutamine
L-methionine D,L-sulfoximine
-
-
L-methionine sulfone
L-methionine-SR-sulfoxide
malate
methionine
Mg2+
-
slight inhibition
NADH
-
substrate inhibition at high concentrations
NADPH
-
0.2 mM, 50% inhibition
O-diazoacetyl-L-serine
-
1 mM, 30 min, 0┬░C, pH 7.6, irreversible inhibition
oxaloacetate
-
weak inhibition, competitive with 2-oxoglutarate
p-hydroxymercuribenzoate
-
complete inhibition at 0.1-1 mM, at 0.0005 mM 33% inhibition or 75% inhibition after preincubation in presence of glutamine and 2-oxoglutarate, substrates induce a conformation, which makes essential sulfhydryl groups more accessible to reagent
phenylalanine
-
20 mM, complete inhibition
pyruvate
serine
Sodium dithionite
-
strong inhibitory effect
tryptophan
xanthine
-
activity of NADH-GOGAT I significantly decreases in the presence of 10 mM xanthine
Zn2+
-
slight inhibition
additional information
-