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1.4.1.14: glutamate synthase (NADH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADH), go to the full flat file.

Word Map on EC 1.4.1.14

Reaction

2 L-glutamate +

NAD+
=
L-glutamine
+
2-oxoglutarate
+
NADH
+
H+

Synonyms

gltA, GltA2, gltB, glutamate (reduced nicotinamide adenine dinucleotide) synthase, glutamate synthase, glutamate synthase (NADH), glutamate synthase (NADH-dependent), glutamate synthetase, glutamine 2-oxoglutarate aminotransferase, GOGAT, GOGAT1, GOGAT2, L-glutamate synthase (NADH), L-glutamate synthetase, NADH glutamate synthase, NADH-dependent glutamate synthase, NADH-dependent glutamine-2-oxoglutarate aminotransferase, NADH-GltS, NADH-glutamate synthase, NADH-GOGAT, NADH-GOGAT I, NADH-GOGAT II, NADH-GOGAT1, NADH-GOGAT2, NADH: GOGAT, Os01g0681900, Os05g0555600, synthase, glutamate (reduced nicotinamide adenine dinucleotide)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.14 glutamate synthase (NADH)

Cloned

Cloned on EC 1.4.1.14 - glutamate synthase (NADH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length cDNA clone and genomic clone for NADH-GOGAT gene
-
gene encoding NADH-GltS is cloned, N-terminal three quarters of the gene product is similar to the bacterial alpha subunit from NADPH-GltS
genes gltA and gltA2, phylogenetic analysis
-
isolation and characterization of NADH-GOGAT gene with 22 exons and 21 introns, isolation of 7.2 kbp cDNA encoding the complete enzyme
-
locus of structural gene encoding NADH-GOGAT is mapped on chromosome 1, detection of quantitative trait loci associated with the enzyme content in leaves
-
NADH-GOGAT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis
NADH-GOGAT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis
open reading frames encoding proteins similar to NADH-GltS are found during sequencing of genome
overexpression of chimeric NADH-GOGAT gene from Medicago sativa fused to the CaMV 35S promoter in transgenic Nicotiana tabacum
-
single copy gene glt1, expression pattern analysis
transgenic plants expressing antisense Fd-GOGAT mRNA, NADH-GOGAT activity remains low
-
transgenic tobacco lines harboring the GLU1 promoter of the Arabidopsis Fd-GOGAT fused to a GUS reporter gene, NADH-GOGAT activity decreases in old leaves by 32% to 35%
-