Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid | potent irreversible inhibitor | Saccharomyces cerevisiae | |
6-diazo-5-oxo-L-norleucine | 0.1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition | Saccharomyces cerevisiae | |
D-glutamine | slight inhibition | Saccharomyces cerevisiae | |
DL-ethionine sulfone | potent competitive inhibitor | Saccharomyces cerevisiae | |
DL-homocysteic acid | - |
Saccharomyces cerevisiae | |
L-2-Amino-4-oxo-5-chloropentanoate | 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition | Saccharomyces cerevisiae | |
L-Albizziin | less inhibitory than L-homocysteine sulfonamide, methionine sulfone and ethionine sulfone | Saccharomyces cerevisiae | |
L-homocysteine sulfonamide | very potent transition state inhibitor, competitive to L-glutamine | Saccharomyces cerevisiae | |
L-methionine sulfone | very potent competitive inhibitor | Saccharomyces cerevisiae | |
L-methionine-SR-sulfoxide | slight inhibition | Saccharomyces cerevisiae | |
O-diazoacetyl-L-serine | 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADH | - |
Saccharomyces cerevisiae | |
0.04 | - |
2-oxoglutarate | - |
Saccharomyces cerevisiae | |
0.28 | - |
L-glutamine | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
169000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
265000 | - |
- |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
7500fold purification | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | low activity when NH3 is substituted for glutamine, NH4Cl: 2.7% of the activity with L-glutamine | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
additional information | no activity with 2-oxovalerate, 2-oxopimelate, 2-oxoadipate, oxaloacetate, D-glutamine, L- or D-asparagine, L- or D-homoglutamine, alpha-methyl-L-glutamine, DL-beta-glutamine, L-isoglutamine, glutaramide or glutaramate | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | glutamine-dependent NADH oxidase activity | Saccharomyces cerevisiae |
9 | - |
ammonia-dependent NADH oxidase activity, rate steadily increases from pH 6 to 9 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Saccharomyces cerevisiae | |
NADH | specific for NADH as reductant | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
L-homocysteine sulfonamide | - |
Saccharomyces cerevisiae | |
0.005 | - |
L-methionine sulfone | - |
Saccharomyces cerevisiae | |
0.015 | - |
DL-ethionine sulfone | - |
Saccharomyces cerevisiae | |
0.1 | - |
L-Albizziin | - |
Saccharomyces cerevisiae | |
0.146 | - |
(2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid | - |
Saccharomyces cerevisiae | |
0.178 | - |
DL-homocysteic acid | - |
Saccharomyces cerevisiae | |
0.3 | - |
D-glutamine | - |
Saccharomyces cerevisiae | |
0.3 | - |
L-methionine-SR-sulfoxide | - |
Saccharomyces cerevisiae |