General Stability | Organism |
---|---|
2-oxoglutarate, 2-mercaptoethanol and EDTA stabilize and have a synergystic effect | Clostridium pasteurianum |
sodium dithionite and glutamine stabilize | Clostridium pasteurianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
asparagine | slight inhibition | Clostridium pasteurianum | |
Co2+ | slight inhibition | Clostridium pasteurianum | |
Cu2+ | Cu2+ and Fe2+ are most inhibitory metal ions | Clostridium pasteurianum | |
Fe2+ | Cu2+ and Fe2+ are most inhibitory metal ions | Clostridium pasteurianum | |
glycine | slight inhibition | Clostridium pasteurianum | |
L-glutamate | 4 mM, 20% inhibition, product inhibition | Clostridium pasteurianum | |
L-histidine | 25 mM, 20-30% inhibition | Clostridium pasteurianum | |
methionine | 25 mM L-methionine, 20-30% inhibition | Clostridium pasteurianum | |
Mg2+ | slight inhibition | Clostridium pasteurianum | |
additional information | not inhibited by Mn2+ and Ca2+ | Clostridium pasteurianum | |
pyruvate | slight inhibition, analogue of 2-oxoglutarate | Clostridium pasteurianum | |
serine | 25 mM L-serine, 20-30% inhibition | Clostridium pasteurianum | |
Sodium dithionite | strong inhibitory effect | Clostridium pasteurianum | |
tryptophan | 25 mM L-tryptophan, 20-30% inhibition | Clostridium pasteurianum | |
Zn2+ | slight inhibition | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NADH | - |
Clostridium pasteurianum | |
0.16 | - |
L-glutamine | - |
Clostridium pasteurianum | |
0.17 | - |
2-oxoglutarate | - |
Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | absence of flavin and iron | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
31000 | - |
2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
68000 | - |
2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
86000 | - |
2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
91000 | - |
2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
590000 | - |
gel filtration | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | Clostridium pasteurianum | regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen | glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | Clostridium pasteurianum | ammonia assimilation | glutamate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
strain ATCC 6013 | - |
Purification (Comment) | Organism |
---|---|
58fold purification | Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
- |
Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | 1 mol 2-oxoglutarate and 1 mol glutamine are consumed per mol NADH oxidized | Clostridium pasteurianum | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Clostridium pasteurianum | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Clostridium pasteurianum | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen | Clostridium pasteurianum | glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | ammonia assimilation | Clostridium pasteurianum | glutamate + NAD+ | - |
? | |
additional information | no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH | Clostridium pasteurianum | ? | - |
? | |
additional information | no activity with albizziin, 6-diazo-5-oxo-L-norvaline, azaserine, methionine sulfoximine, FADH or FMNH | Clostridium pasteurianum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE | Clostridium pasteurianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Clostridium pasteurianum |
50 | - |
- |
Clostridium pasteurianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
enzyme loses activity after incubation for 10 min | Clostridium pasteurianum |
50 | - |
half-life: 10 min, presence of 2-oxoglutarate and L-glutamine stabilizes against thermal denaturation, whereas preincubation with NADH or L-glutamate causes more heat sensitivity | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
broad pH profile with optimum at pH 7.2 | Clostridium pasteurianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Clostridium pasteurianum | |
additional information | absence of flavin and iron | Clostridium pasteurianum | |
NADH | specific for NADH as reductant | Clostridium pasteurianum |