Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 5 mM, stimulates activity with 36% enhancement | Prosthecochloris vibrioformis f. thiosulfatophilum | |
succinate | 50 mM, stimulates activity by 57% | Prosthecochloris vibrioformis f. thiosulfatophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 20 mM, about 75% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
alanine | slight inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
AMP | 20 mM, about 75% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
arginine | 20 mM, complete inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
Atebrin | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | |
ATP | 5 mM, 75% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
glycine | 48% inhibition at 10 mM, complete inhibition at 20 mM | Prosthecochloris vibrioformis f. thiosulfatophilum | |
leucine | 10 mM, 52% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
malate | 20 mM, 30% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
methionine | 10 mM, 72% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
additional information | cumulative inhibition by combinations of amino acids and nucleotides, cumulative feedback inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
NAD+ | 1 mM, 27% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
NADP+ | 1 mM, 48% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
phenylalanine | 20 mM, complete inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
proline | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | |
pyruvate | 20 mM, 20% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
serine | 10 mM, 73% inhibition | Prosthecochloris vibrioformis f. thiosulfatophilum | |
tryptophan | 29% inhibition at 10 mM, complete inhibition at 20 mM | Prosthecochloris vibrioformis f. thiosulfatophilum | |
valine | - |
Prosthecochloris vibrioformis f. thiosulfatophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADH | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | |
0.27 | - |
2-oxoglutarate | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | |
0.769 | - |
L-glutamine | - |
Prosthecochloris vibrioformis f. thiosulfatophilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatophore | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | Prosthecochloris vibrioformis f. thiosulfatophilum | glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | Prosthecochloris vibrioformis f. thiosulfatophilum | key enzyme for glutamate production | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | key enzyme for glutamate production | glutamate + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prosthecochloris vibrioformis f. thiosulfatophilum | - |
- |
- |
Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
222fold purification | Prosthecochloris vibrioformis f. thiosulfatophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Prosthecochloris vibrioformis f. thiosulfatophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Prosthecochloris vibrioformis f. thiosulfatophilum | L-glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Prosthecochloris vibrioformis f. thiosulfatophilum | L-glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | L-glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | L-glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia | Prosthecochloris vibrioformis f. thiosulfatophilum | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | key enzyme for glutamate production | Prosthecochloris vibrioformis f. thiosulfatophilum | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | glutamine synthetase/glutamate synthase pathway for ammonia assimilation is fafavoured by growth at low concentrations of ammonia | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | glutamate + NAD+ | - |
ir | |
L-glutamine + 2-oxoglutarate + NADH | key enzyme for glutamate production | Prosthecochloris vibrioformis f. thiosulfatophilum NCIB 8346 | glutamate + NAD+ | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Prosthecochloris vibrioformis f. thiosulfatophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavin component | Prosthecochloris vibrioformis f. thiosulfatophilum | |
NADH | specific for NADH as reductant | Prosthecochloris vibrioformis f. thiosulfatophilum |