1.14.11.11: hyoscyamine (6S)-dioxygenase

This is an abbreviated version!
For detailed information about hyoscyamine (6S)-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.11

Reaction

L-hyoscyamine
+
2-oxoglutarate
+
O2
=
(6S)-hydroxyhyoscyamine
+
succinate
+
CO2

Synonyms

6H6, AaH6H, AtH6H, H6H, HnH6H, hyoscyamine 6-hydroxylase, hyoscyamine 6beta-hydroxylase, hyoscyamine-6beta-hydroxylase, oxygenase, hyoscyamine 6beta-di-

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.11 hyoscyamine (6S)-dioxygenase

Reference

Reference on EC 1.14.11.11 - hyoscyamine (6S)-dioxygenase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hashimoto, T.; Yamada, Y.
Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. Hydroxylase and epoxidase activities in the enzyme preparation
Eur. J. Biochem.
164
277-285
1987
Hyoscyamus niger
Manually annotated by BRENDA team
Matsuda, J.; Okabe, S.; Hashimoto, T.; Yamada, Y.
Molecular cloning of hyoscyamine 6 beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger
J. Biol. Chem.
266
9460-9464
1991
Hyoscyamus niger
Manually annotated by BRENDA team
Hashimoto, T.; Yamada, Y.
Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, an alkaloid-producing root cultures
Plant Physiol.
81
619-625
1986
Atropa belladonna, Datura metel, Duboisia leichhardtii, Hyoscyamus albus, Hyoscyamus gyorffi, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus pusillus
Manually annotated by BRENDA team
Rocha, P.; Stenzel, O.; Parr, A.; Walton, N.; Christou, P.; Drager, B.; Leech, M.J.
Functional expression of tropinone reductase I (trI) and hyoscyamine-6beta-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum
Plant Sci.
162
905-913
2002
Hyoscyamus niger
-
Manually annotated by BRENDA team
Yun, D.J.; Hashimoto, T.; Yamada, Y.
Transgenic tobacco plants with two consecutive oxidation reactions catalyzed by hyoscyamine 6b-hydroxylase
Biosci. Biotechnol. Biochem.
57
502-503
1993
Hyoscyamus niger
-
Manually annotated by BRENDA team
Suzuki, K.I.; Yun, D.J.; Chen, X.Y.; Yamada, Y.; Hashimoto, T.
An Atropa belladonna hyoscyamine 6beta-hydroxylase gene is differentially expressed in the root pericycle and anthers
Plant Mol. Biol.
40
141-152
1999
Atropa belladonna
Manually annotated by BRENDA team
Matsuda, J.; Hashimoto, T.; Yamada, Y.
Analysis of active-site residues in hyoscyamine 6beta-hydroxylase
Plant Biotechnol.
14
51-57
1997
Hyoscyamus niger
-
Manually annotated by BRENDA team
Palazon, J.; Moyano, E.; Cusido, R.M.; Bonfill, M.; Oksman-Caldentey, K.M.; Pinol, M.T.
Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene
Plant Sci.
165
1289-1295
2003
Hyoscyamus niger
-
Manually annotated by BRENDA team
Kang, S.M.; Jung, H.Y.; Kang, Y.M.; Yun, D.J.; Bahk, J.D.; Yang, J.k.; Choi, M.S.
Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora
Plant Sci.
166
745-751
2004
Scopolia parviflora
Manually annotated by BRENDA team
Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; He, H.X.
Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus
Planta Med.
71
249-253
2005
Anisodus tanguticus (Q6V0J1), Anisodus tanguticus
Manually annotated by BRENDA team
Zarate, R.; el Jaber-Vazdekis, N.; Medina, B.; Ravelo, A.G.
Tailoring tropane alkaloid accumulation in transgenic hairy roots of Atropa baetica by over-expressing the gene encoding hyoscyamine 6beta-hydroxylase
Biotechnol. Lett.
28
1271-1277
2006
Hyoscyamus niger
Manually annotated by BRENDA team
Rahman, L.u.; Kitamura, Y.; Yamaguchi, J.; Mukai, M.; Akiyama, K.; Yamamoto, H.; Muranaka, T.; Ikenaga, T.
Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production
Enzyme Microb. Technol.
39
1183-1189
2006
Hyoscyamus niger
-
Manually annotated by BRENDA team
Haekkinen, S.T.; Moyano, E.; Cusido, R.M.; Palazon, J.; Pinol, M.T.; Oksman-Caldentey, K.M.
Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase
J. Exp. Bot.
56
2611-2618
2005
Hyoscyamus niger
Manually annotated by BRENDA team
Kai, G.; Chen, J.; Li, L.; Zhou, G.; Zhou, L.; Zhang, L.; Chen, Y.; Zhao, L.
Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6beta-hydroxylase from roots of Anisodus acutangulus
J. Biochem. Mol. Biol.
40
715-722
2007
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
Manually annotated by BRENDA team
Cardillo, A.B.; Talou, J.R.; Giulietti, A.M.
Expression of Brugmansia candida hyoscyamine 6beta-hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst
Microb. Cell Fact.
7
17
2008
Brugmansia x candida (B2CNC8)
Manually annotated by BRENDA team
Higa, A.; Miyamoto, E.; Rahman, L.u.; Kitamura, Y.
Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency
Plant Physiol. Biochem.
46
452-460
2008
Hyoscyamus albus
Manually annotated by BRENDA team
Jaber-Vazdekis, N.; Gonzalez, C.; Ravelo, A.G.; Zarate, R.
Cloning, characterization and analysis of expression profiles of a cDNA encoding a hyoscyamine 6beta-hydroxylase (H6H) from Atropa baetica Willk
Plant Physiol. Biochem.
47
20-25
2009
Atropa baetica (A9Q1G4), Atropa baetica
Manually annotated by BRENDA team
Pramod, K.K.; Singh, S.; Jayabaskaran, C.
Biochemical and structural characterization of recombinant hyoscyamine 6beta-hydroxylase from Datura metel L
Plant Physiol. Biochem.
48
966-970
2010
Datura metel
Manually annotated by BRENDA team
Li, J.; van Belkum, M.J.; Vederas, J.C.
Functional characterization of recombinant hyoscyamine 6beta-hydroxylase from Atropa belladonna
Bioorg. Med. Chem.
20
4356-4363
2012
Atropa belladonna
Manually annotated by BRENDA team
Kai, G.; Liu, Y.; Wang, X.; Yang, S.; Fu, X.; Luo, X.; Liao, P.
Functional identification of hyoscyamine 6beta-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
Biotechnol. Lett.
33
1361-1365
2011
Anisodus acutangulus
Manually annotated by BRENDA team
Kai, G.; Zhang, A.; Guo, Y.; Li, L.; Cui, L.; Luo, X.; Liu, C.; Xiao, J.
Enhancing the production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by over-expressing tropinone reductase I and hyoscyamine-6beta-hydroxylase
Mol. Biosyst.
8
2883-2890
2012
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
Manually annotated by BRENDA team
Vakili, B.; Karimi, F.; Sharifi, M.; Behmanesh, M.
Chromium-induced tropane alkaloid production and H6H gene expression in Atropa belladonna L. (Solanaceae) in vitro-propagated plantlets
Plant Physiol. Biochem.
52
98-103
2012
Atropa belladonna (Q9XJ43)
Manually annotated by BRENDA team
Dehghan, E.; Shahriari Ahmadi, F.; Ghotbi Ravandi, E.; Reed, D.W.; Covello, P.S.; Bahrami, A.R.
An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis
Plant Physiol. Biochem.
70
188-194
2013
Hyoscyamus senecionis (J7HDC2), Hyoscyamus senecionis
Manually annotated by BRENDA team
Cardillo, A.; Perassolo, M.; Sartuqui, M.; Rodri­guez Talou, J.; Giulietti, A.
Production of tropane alkaloids by biotransformation using recombinant Escherichia coli whole cells
Biochem. Eng. J.
125
180-189
2017
Brugmansia x candida (B2CNC8)
-
Manually annotated by BRENDA team
Cao, Y.D.; He, Y.C.; Li, H.; Kai, G.Y.; Xu, J.H.; Yu, H.L.
Efficient biosynthesis of rare natural product scopolamine using E. coli cells expressing a S14P/K97A mutant of hyoscyamine 6beta-hydroxylase AaH6H
J. Biotechnol.
211
123-129
2015
Anisodus acutangulus (A2IBI0), Anisodus acutangulus
Manually annotated by BRENDA team
Xia, K.; Liu, X.; Zhang, Q.; Qiang, W.; Guo, J.; Lan, X.; Chen, M.; Liao, Z.
Promoting scopolamine biosynthesis in transgenic Atropa belladonna plants with pmt and h6h overexpression under field conditions
Plant Physiol. Biochem.
106
46-53
2016
Hyoscyamus niger (P24397), Hyoscyamus niger
Manually annotated by BRENDA team