Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a fusion protein to maltose-binding protein | Hyoscyamus niger |
Protein Variants | Comment | Organism |
---|---|---|
D219H | inactive mutant enzyme | Hyoscyamus niger |
D219N | inactive mutant enzyme | Hyoscyamus niger |
H217Q | inactive mutant enzyme | Hyoscyamus niger |
H66Q | mutant enzyme has 97% of the activity of the wild-type enzyme | Hyoscyamus niger |
S274Q | inactive mutant enzyme | Hyoscyamus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? |