BRENDA - Enzyme Database show
show all sequences of 1.14.11.11

Analysis of active-site residues in hyoscyamine 6beta-hydroxylase

Matsuda, J.; Hashimoto, T.; Yamada, Y.; Plant Biotechnol. 14, 51-57 (1997)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli as a fusion protein to maltose-binding protein
Hyoscyamus niger
Engineering
Amino acid exchange
Commentary
Organism
D219H
inactive mutant enzyme
Hyoscyamus niger
D219N
inactive mutant enzyme
Hyoscyamus niger
H217Q
inactive mutant enzyme
Hyoscyamus niger
H66Q
mutant enzyme has 97% of the activity of the wild-type enzyme
Hyoscyamus niger
S274Q
inactive mutant enzyme
Hyoscyamus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyoscyamus niger
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-hyoscyamine + 2-oxoglutarate + O2
-
439281
Hyoscyamus niger
6beta-hydroxyhyoscyamine + succinate + CO2
-
439281
Hyoscyamus niger
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli as a fusion protein to maltose-binding protein
Hyoscyamus niger
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D219H
inactive mutant enzyme
Hyoscyamus niger
D219N
inactive mutant enzyme
Hyoscyamus niger
H217Q
inactive mutant enzyme
Hyoscyamus niger
H66Q
mutant enzyme has 97% of the activity of the wild-type enzyme
Hyoscyamus niger
S274Q
inactive mutant enzyme
Hyoscyamus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-hyoscyamine + 2-oxoglutarate + O2
-
439281
Hyoscyamus niger
6beta-hydroxyhyoscyamine + succinate + CO2
-
439281
Hyoscyamus niger
?
Other publictions for EC 1.14.11.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741836
Cardillo
-
Production of tropane alkaloi ...
Brugmansia x candida
Biochem. Eng. J.
125
180-189
2017
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1
1
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743509
Xia
Promoting scopolamine biosynt ...
Hyoscyamus niger
Plant Physiol. Biochem.
106
46-53
2016
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742949
Cao
Efficient biosynthesis of rar ...
Anisodus acutangulus
J. Biotechnol.
211
123-129
2015
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1
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2
2
726207
Dehghan
An atypical pattern of accumul ...
Hyoscyamus senecionis
Plant Physiol. Biochem.
70
188-194
2013
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1
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6
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2
1
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1
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2
2
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724556
Li
Functional characterization of ...
Atropa belladonna
Bioorg. Med. Chem.
20
4356-4363
2012
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1
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8
1
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2
2
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1
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1
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1
1
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1
1
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1
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1
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725946
Kai
Enhancing the production of tr ...
Anisodus acutangulus
Mol. Biosyst.
8
2883-2890
2012
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1
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1
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1
1
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726202
Vakili
Chromium-induced tropane alkal ...
Atropa belladonna
Plant Physiol. Biochem.
52
98-103
2012
1
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3
3
1
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724637
Kai
Functional identification of h ...
Anisodus acutangulus
Biotechnol. Lett.
33
1361-1365
2011
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713300
Pramod
Biochemical and structural cha ...
Datura metel
Plant Physiol. Biochem.
48
966-970
2010
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1
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2
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1
1
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700788
Jaber-Vazdekis
Cloning, characterization and ...
Atropa baetica
Plant Physiol. Biochem.
47
20-25
2009
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1
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688836
Cardillo
Expression of Brugmansia candi ...
Brugmansia x candida
Microb. Cell Fact.
7
17
2008
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1
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1
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700783
Higa
Root tip-dependent, active rib ...
Hyoscyamus albus
Plant Physiol. Biochem.
46
452-460
2008
-
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1
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687475
Kai
Molecular cloning and characte ...
Anisodus acutangulus
J. Biochem. Mol. Biol.
40
715-722
2007
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1
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5
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4
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4
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1
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1
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672740
Zarate
Tailoring tropane alkaloid acc ...
Hyoscyamus niger
Biotechnol. Lett.
28
1271-1277
2006
-
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1
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673384
Rahman
-
Exogenous plant H6H but not ba ...
Hyoscyamus niger
Enzyme Microb. Technol.
39
1183-1189
2006
-
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1
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660331
Liu
Molecular cloning, expression ...
Anisodus tanguticus
Planta Med.
71
249-253
2005
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1
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8
2
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1
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4
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675125
Haekkinen
Enhanced secretion of tropane ...
Hyoscyamus niger
J. Exp. Bot.
56
2611-2618
2005
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660281
Kang
-
Effects of methyl jasmonate an ...
Scopolia parviflora
Plant Sci.
166
745-751
2004
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660278
Palazon
-
Alkaloid production in Duboisi ...
Hyoscyamus niger
Plant Sci.
165
1289-1295
2003
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439278
Rocha
-
Functional expression of tropi ...
Hyoscyamus niger
Plant Sci.
162
905-913
2002
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439280
Suzuki
An Atropa belladonna hyoscyami ...
Atropa belladonna
Plant Mol. Biol.
40
141-152
1999
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439281
Matsuda
-
Analysis of active-site residu ...
Hyoscyamus niger
Plant Biotechnol.
14
51-57
1997
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5
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439279
Yun
-
Transgenic tobacco plants with ...
Hyoscyamus niger
Biosci. Biotechnol. Biochem.
57
502-503
1993
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439276
Matsuda
Molecular cloning of hyoscyami ...
Hyoscyamus niger
J. Biol. Chem.
266
9460-9464
1991
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439275
Hashimoto
Purification and characterizat ...
Hyoscyamus niger
Eur. J. Biochem.
164
277-285
1987
6
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31
3
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15
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439277
Hashimoto
Hyoscyamine 6beta-hydroxylase, ...
Atropa belladonna, Datura metel, Duboisia leichhardtii, Hyoscyamus albus, Hyoscyamus gyorffi, Hyoscyamus muticus, Hyoscyamus niger, Hyoscyamus pusillus
Plant Physiol.
81
619-625
1986
32
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2
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8
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8
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1
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8
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32
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2
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8
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8
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1
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8
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8
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