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Literature summary for 1.14.11.11 extracted from

  • Kai, G.; Zhang, A.; Guo, Y.; Li, L.; Cui, L.; Luo, X.; Liu, C.; Xiao, J.
    Enhancing the production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by over-expressing tropinone reductase I and hyoscyamine-6beta-hydroxylase (2012), Mol. Biosyst., 8, 2883-2890.
    View publication on PubMed

Application

Application Comment Organism
pharmacology tropane alkaloids including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, improvement of production by metabolic engineering introduction of genes encoding the branch-controlling enzyme tropinone reductase I and the downstream rate-limiting enzyme hyoscyamine-6beta-hydroxylase Anisodus acutangulus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the enzyme in hairy roots of transgenic Anisodus acutangulus using Agrobacterium tumefaciens strain C58C1 transfection method, coexpression with tropinone reductase I Anisodus acutangulus

Protein Variants

Protein Variants Comment Organism
additional information enhanced production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropan alkaloid contents in transgenic lines, overview Anisodus acutangulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2 Anisodus acutangulus
-
(6S)-hydroxyhyoscyamine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Anisodus acutangulus A2IBI0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
Anisodus acutangulus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2
-
Anisodus acutangulus (6S)-hydroxyhyoscyamine + succinate + CO2
-
?
additional information the enzyme also catalyzes the reaction of EC 1.14.11.14 Anisodus acutangulus ?
-
?

Synonyms

Synonyms Comment Organism
H6H
-
Anisodus acutangulus
hyoscyamine-6beta-hydroxylase
-
Anisodus acutangulus

General Information

General Information Comment Organism
metabolism the enzyme is involved in biosynthesis pathway of tropane alkaloids catalyzing two oxidation steps to form scopolamine from hyoscyamine, overview, cf. EC 1.14.11.14 Anisodus acutangulus