1.13.11.66: hydroquinone 1,2-dioxygenase

This is an abbreviated version!
For detailed information about hydroquinone 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.66

Reaction

benzene-1,4-diol
+
O2
=
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate

Synonyms

HQDO, hydroquinone 1,2-dioxygenase, hydroquinone dioxygenase, LinE, mnpC, PcpA, PdcDE, PnpC1, PnpC2, PnpCD, two-subunit hydroquinone 1,2-dioxygenase, type II HQDO, YaiA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.66 hydroquinone 1,2-dioxygenase

Purification

Purification on EC 1.13.11.66 - hydroquinone 1,2-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
C1I210; C1I209
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate
-
using Ni-NTA chromatography
-