1.13.11.66: hydroquinone 1,2-dioxygenase

This is an abbreviated version!
For detailed information about hydroquinone 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.66

Reaction

benzene-1,4-diol
+
O2
=
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate

Synonyms

HQDO, hydroquinone 1,2-dioxygenase, hydroquinone dioxygenase, LinE, mnpC, PcpA, PdcDE, PnpC1, PnpC2, PnpCD, two-subunit hydroquinone 1,2-dioxygenase, type II HQDO, YaiA

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.66 hydroquinone 1,2-dioxygenase

Engineering

Engineering on EC 1.13.11.66 - hydroquinone 1,2-dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E248Q
C1I210; C1I209
site-directed mutagenesis, the mutation results in complete loss of enzyme activity
F264A
C1I210; C1I209
site-directed mutagenesis, the mutation results in almost complete loss of enzyme activity
F79A
C1I210; C1I209
site-directed mutagenesis, the mutation results in almost complete loss of enzyme activity
L252A
C1I210; C1I209
site-directed mutagenesis, the mutation results in a 70% loss of enzyme activity
L313A
C1I210; C1I209
site-directed mutagenesis, the mutation results in almost complete loss of enzyme activity
V315A
C1I210; C1I209
site-directed mutagenesis, the mutation results in a 50% loss of enzyme activity
W230A
C1I210; C1I209
site-directed mutagenesis, the mutation results in a 70% loss of enzyme activity
W273a
C1I210; C1I209
site-directed mutagenesis, the mutation results in almost complete loss of enzyme activity
W76A
C1I210; C1I209
site-directed mutagenesis, the mutation results in almost complete loss of enzyme activity
E276A
-
less than 6% of wild-type activity
H11A
-
less than 6% of wild-type activity
H159A
-
67% of wild-type activity
H227A
-
less than 6% of wild-type activity
Y266F
-
about 6% of wild-type activity
E278A
mutation in putative Fe(II)-binding site, complete loss of activity
H162A
mutation in putative Fe(II)-binding site, complete loss of activity
H229A
mutation in putative Fe(II)-binding site, complete loss of activity
additional information