BRENDA - Enzyme Database
show all sequences of 1.13.11.66

A copper-induced quinone degradation pathway provides protection against combined copper/quinone stress in Lactococcus lactis IL1403

Mancini, S.; Abicht, H.K.; Gonskikh, Y.; Solioz, M.; Mol. Microbiol. 95, 645-659 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene yaiA, encoded in the the yahCD-yaiAB operon, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli. YaiA activity could be detected in crude Escherichia coli cytoplasmic extract overexpressing Lactcoccus lactis YaiA from a plasmid
Lactococcus lactis
General Stability
General Stability
Organism
4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme
Lactococcus lactis
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme
Lactococcus lactis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
2-methylhydroquinone
pH 6.4, 30°C, recombinant enzyme
Lactococcus lactis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
copper
copper content is 0.12 atoms per monomer
Lactococcus lactis
Iron
iron content is 0.96 atoms per monomer
Lactococcus lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-methylhydroquinone + O2
Lactococcus lactis
-
?
-
-
?
2-methylhydroquinone + O2
Lactococcus lactis IL1403
-
?
-
-
?
benzene-1,4-diol + O2
Lactococcus lactis
-
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
?
benzene-1,4-diol + O2
Lactococcus lactis IL1403
-
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
?
additional information
Lactococcus lactis
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
?
-
-
-
additional information
Lactococcus lactis IL1403
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis IL1403
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,5-dimethylhydroquinone + O2
-
745802
Lactococcus lactis
2-methylmaleylacetone
-
-
-
?
2,5-dimethylhydroquinone + O2
-
745802
Lactococcus lactis IL1403
2-methylmaleylacetone
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis
?
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis IL1403
?
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis
maleylacetone
-
-
-
?
benzene-1,4-diol + O2
-
745802
Lactococcus lactis
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
-
?
benzene-1,4-diol + O2
-
745802
Lactococcus lactis IL1403
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
-
?
additional information
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
745802
Lactococcus lactis
?
-
-
-
-
additional information
2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone
745802
Lactococcus lactis
?
-
-
-
-
additional information
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
745802
Lactococcus lactis IL1403
?
-
-
-
-
additional information
2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone
745802
Lactococcus lactis IL1403
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 43300, SDS-PAGE
Lactococcus lactis
Synonyms
Synonyms
Commentary
Organism
hydroquinone dioxygenase
-
Lactococcus lactis
YaiA
-
Lactococcus lactis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lactococcus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
with substrate 2-methylhydroquinone
Lactococcus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene yaiA, encoded in the the yahCD-yaiAB operon, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli. YaiA activity could be detected in crude Escherichia coli cytoplasmic extract overexpressing Lactcoccus lactis YaiA from a plasmid
Lactococcus lactis
General Stability (protein specific)
General Stability
Organism
4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme
Lactococcus lactis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
4-hydrobenzoate, the competitive inhibitor of the heterotetrameric HapCD hydroquinone dioxygenase of Pseudomonas fluorescens ACB, stabilizes the enzyme
Lactococcus lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
2-methylhydroquinone
pH 6.4, 30°C, recombinant enzyme
Lactococcus lactis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
copper
copper content is 0.12 atoms per monomer
Lactococcus lactis
Iron
iron content is 0.96 atoms per monomer
Lactococcus lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-methylhydroquinone + O2
Lactococcus lactis
-
?
-
-
?
2-methylhydroquinone + O2
Lactococcus lactis IL1403
-
?
-
-
?
benzene-1,4-diol + O2
Lactococcus lactis
-
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
?
benzene-1,4-diol + O2
Lactococcus lactis IL1403
-
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
?
additional information
Lactococcus lactis
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
?
-
-
-
additional information
Lactococcus lactis IL1403
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,5-dimethylhydroquinone + O2
-
745802
Lactococcus lactis
2-methylmaleylacetone
-
-
-
?
2,5-dimethylhydroquinone + O2
-
745802
Lactococcus lactis IL1403
2-methylmaleylacetone
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis
?
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis IL1403
?
-
-
-
?
2-methylhydroquinone + O2
-
745802
Lactococcus lactis
maleylacetone
-
-
-
?
benzene-1,4-diol + O2
-
745802
Lactococcus lactis
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
-
?
benzene-1,4-diol + O2
-
745802
Lactococcus lactis IL1403
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate
-
-
-
?
additional information
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
745802
Lactococcus lactis
?
-
-
-
-
additional information
2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone
745802
Lactococcus lactis
?
-
-
-
-
additional information
YaiA is a hydroquinone dioxygenase that converts hydroquinone putatively to 4-hydroxymuconic semialdehyde in an oxygen-consuming reaction. Hydroquinone and methylhydroquinone are both substrates of YaiA
745802
Lactococcus lactis IL1403
?
-
-
-
-
additional information
2-methylhydroquinone is a slightly better substrate than unsubstituted hydroquinone
745802
Lactococcus lactis IL1403
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43300, SDS-PAGE
Lactococcus lactis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lactococcus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
with substrate 2-methylhydroquinone
Lactococcus lactis
Expression
Organism
Commentary
Expression
Lactococcus lactis
the the yahCD-yaiAB operon is induced by copper but not by quinones
up
General Information
General Information
Commentary
Organism
physiological function
the enzyme YaiA plays a key role in alleviating quinone/copper stress. The yahCD-yaiAB operon of Lactococcus lactis IL1403 provides resistance to combined copper/quinone stress. The operon is under the control of CopR, which also regulates expression of the copRZA copper resistance operon as well as other Lactococcus lactis genes. Expression of the yahCD-yaiAB operon is induced by copper but not by quinones. Copper induction of the yahCD-yaiAB operon offers protection to copper/quinone toxicity and can provide a growth advantage to Lactococcus lactis in some environments. Regulation of quinone degradation by CopR thus appears to be a hallmark of Lactococci. The conversion of hydroquinone to 4-hydroxymuconic semialdehyde by the YaiA dioxygenase appears to be too slow for acute detoxification, but probably serves as long-term remedy by degrading the hydroquinone pool, which constitutes a reservoir for the formation of toxic pbenzoquinones by spontaneous oxidation that is greatly accelerated by copper
Lactococcus lactis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme YaiA plays a key role in alleviating quinone/copper stress. The yahCD-yaiAB operon of Lactococcus lactis IL1403 provides resistance to combined copper/quinone stress. The operon is under the control of CopR, which also regulates expression of the copRZA copper resistance operon as well as other Lactococcus lactis genes. Expression of the yahCD-yaiAB operon is induced by copper but not by quinones. Copper induction of the yahCD-yaiAB operon offers protection to copper/quinone toxicity and can provide a growth advantage to Lactococcus lactis in some environments. Regulation of quinone degradation by CopR thus appears to be a hallmark of Lactococci. The conversion of hydroquinone to 4-hydroxymuconic semialdehyde by the YaiA dioxygenase appears to be too slow for acute detoxification, but probably serves as long-term remedy by degrading the hydroquinone pool, which constitutes a reservoir for the formation of toxic pbenzoquinones by spontaneous oxidation that is greatly accelerated by copper
Lactococcus lactis
Expression (protein specific)
Organism
Commentary
Expression
Lactococcus lactis
the the yahCD-yaiAB operon is induced by copper but not by quinones
up
Other publictions for EC 1.13.11.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744430
Ferraroni
The crystal structures of nat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Biochim. Biophys. Acta
1865
520-530
2017
-
-
-
1
-
-
2
-
-
1
-
2
-
5
-
-
-
1
-
-
-
-
6
2
2
1
-
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-
1
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1
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-
2
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1
-
2
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-
6
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
745331
Liu
Crystal structure of PnpCD, a ...
Pseudomonas sp.
J. Biol. Chem.
290
24547-24560
2015
-
-
1
1
9
-
-
-
-
3
-
2
-
1
-
-
1
-
-
-
-
-
2
2
2
1
-
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-
1
-
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1
-
1
9
-
-
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-
3
-
2
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
745802
Mancini
A copper-induced quinone degr ...
Lactococcus lactis, Lactococcus lactis IL1403
Mol. Microbiol.
95
645-659
2015
-
-
1
-
-
1
1
1
-
2
-
6
-
13
-
-
1
-
-
-
-
-
11
1
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
2
-
6
-
-
-
1
-
-
-
-
11
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp.
BMC Microbiol.
12
27
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
-
-
-
2
-
2
1
1
1
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
1
1
1
-
1
1
2
-
-
-
-
-
-
-
718555
Kolvenbach
Purification and characterizat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
AMB Express
1
08
2011
-
-
-
-
-
-
9
1
-
1
3
-
-
7
-
-
1
-
-
-
1
-
32
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
2
-
1
6
-
-
-
-
2
-
-
2
-
32
2
-
-
-
2
-
2
-
-
-
-
-
-
1
2
718916
Machonkin
Substrate specificity of Sphin ...
Sphingobium chlorophenolicum
Biochemistry
50
8899-8913
2011
-
-
-
-
-
-
3
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
9
-
-
-
-
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-
-
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-
-
-
-
-
-
3
-
12
-
-
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-
-
-
-
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-
-
-
-
8
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
719104
Shen
Cloning and characterization o ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Biores. Technol.
101
7516-7522
2010
-
-
-
-
1
-
-
-
-
-
2
-
-
6
-
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4
1
2
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2
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2
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-
4
2
-
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-
-
-
-
-
-
-
-
719327
Yin
Characterization of MnpC, a hy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Curr. Microbiol.
61
471-476
2010
-
-
1
-
-
-
-
1
-
2
1
-
-
27
-
-
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-
1
3
4
1
1
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1
1
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1
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1
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2
1
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1
3
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
720066
Machonkin
Determination of the active si ...
Sphingobium chlorophenolicum
J. Biol. Inorg. Chem.
15
291-301
2010
-
-
1
1
5
-
-
-
-
1
-
-
-
1
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-
-
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1
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1
-
1
5
-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
719714
Moonen
Hydroquinone dioxygenase from ...
Pseudomonas fluorescens, Pseudomonas fluorescens ACB
J. Bacteriol.
190
5199-5209
2008
1
-
-
-
-
-
12
1
-
1
3
-
-
11
-
-
1
-
-
-
1
-
14
1
-
-
-
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
12
1
1
-
1
3
-
-
-
-
1
-
-
1
-
14
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
718727
Xun
Characterization of 2,6-dichlo ...
Sphingobium chlorophenolicum
Biochem. Biophys. Res. Commun.
266
322-325
1999
-
-
1
-
-
-
-
1
-
1
-
-
-
1
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2
-
1
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1
1
1
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1
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1
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1
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-
-
2
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
718831
Xu
Evidence that pcpA encodes 2,6 ...
Sphingobium chlorophenolicum
Biochemistry
38
7659-7669
1999
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
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719693
Miyauchi
Cloning and sequencing of a no ...
Sphingomonas paucimobilis
J. Bacteriol.
181
6712-6719
1999
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