Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tagged fusion proteins | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroquinone + O2 | - |
Pseudomonas sp. | 4-hydroxymuconic acid semialdehyde | - |
? | |
hydroquinone + O2 | - |
Pseudomonas sp. 1-7 | 4-hydroxymuconic acid semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hydroquinone 1,2-dioxygenase | - |
Pseudomonas sp. |
PdcDE | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the purified enzyme retains 35% activity after 20 min at 60°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | - |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
20% activity after 30 min at pH 3.0 | Pseudomonas sp. |
10 | - |
60% activity after 30 min at pH 10.0 | Pseudomonas sp. |