BRENDA - Enzyme Database
show all sequences of 1.13.11.66

Hydroquinone dioxygenase from Pseudomonas fluorescens ACB: a novel member of the family of nonheme-iron(II)-dependent dioxygenases

Moonen, M.J.; Synowsky, S.A.; van den Berg, W.A.; Westphal, A.H.; Heck, A.J.; van den Heuvel, R.H.; Fraaije, M.W.; van Berkel, W.J.; J. Bacteriol. 190, 5199-5209 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
Pseudomonas fluorescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
inactivation
Pseudomonas fluorescens
4-hydroxybenzoate
competitive with hydroquinone
Pseudomonas fluorescens
4-Hydroxybenzonitrile
0.2 mM, no residual activity
Pseudomonas fluorescens
4-hydroxycinnamate
0.2 mM, no residual activity
Pseudomonas fluorescens
4-nitrophenol
0.2 mM, no residual activity
Pseudomonas fluorescens
bromohydroquinone
substrate inhibition
Pseudomonas fluorescens
chlorohydroquinone
substrate inhibition
Pseudomonas fluorescens
hydrogen peroxide
inactivation
Pseudomonas fluorescens
Hydroxyhydroquinone
0.2 mM, 9% residual activity
Pseudomonas fluorescens
methoxyhydroquinone
strong substrtae inhibition
Pseudomonas fluorescens
additional information
weak or no inhibition: 2-hydroxy-, 3-hydroxy-, 2,3-dihydroxy-, 2,5-dihydroxy-, 2,6-dihydroxy-, 3,4-dihydroxy-, 3,4,5-trihydroxy-, 3-chloro-4-hydroxy-, tetrafluoro-4-hydroxy-, 3-amino-4-hydroxy-, 4-hydroxy-3-methoxy-, 4-amino- and methyl 4-hydroxybenzoate; 6-hydroxynicotinate, 4-hydroxypropiophenone, 4-hydroxymandelate, 4-hydroxyphenylglycine, 4-hydroxybenzenesulfonic acid, and 4-methyl-, 4-methoxy-, and 4-aminophenol
Pseudomonas fluorescens
o-phenanthroline
inactivation
Pseudomonas fluorescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
hydroquinone
pH 7.0, 25°C
Pseudomonas fluorescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
10fold activation by Fe2+, each subunit binds one molecule of 4-hydroxybenzoate and one molecule of iron
Pseudomonas fluorescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17800
-
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
38300
-
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
112000
-
gel filtration
Pseudomonas fluorescens
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens ACB
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.89
-
pH 7.0, 25°C
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,3-difluorohydroquinone + O2
80% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
2,3-difluorohydroquinone + O2
80% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
2,5-difluorohydroquinone + O2
75% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
2,5-difluorohydroquinone + O2
75% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
3,5-difluorohydroquinone + O2
90% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
3,5-difluorohydroquinone + O2
90% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
bromohydroquinone + O2
30% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
bromohydroquinone + O2
30% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
chlorohydroquinone + O2
70% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
hydroquinone + O2
-
719714
Pseudomonas fluorescens
4-hydroxymuconic semialdehyde
-
-
-
?
methoxyhydroquinone + O2
50% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
methylhydroquinone + O2
120% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
additional information
no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol
719714
Pseudomonas fluorescens
?
-
-
-
?
additional information
no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1
-
hydroquinone
pH 7.0, 25°C
Pseudomonas fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Pseudomonas fluorescens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.014
-
4-hydroxybenzoate
pH 7.0, 25°C
Pseudomonas fluorescens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
inactivation
Pseudomonas fluorescens
4-hydroxybenzoate
competitive with hydroquinone
Pseudomonas fluorescens
4-Hydroxybenzonitrile
0.2 mM, no residual activity
Pseudomonas fluorescens
4-hydroxycinnamate
0.2 mM, no residual activity
Pseudomonas fluorescens
4-nitrophenol
0.2 mM, no residual activity
Pseudomonas fluorescens
bromohydroquinone
substrate inhibition
Pseudomonas fluorescens
chlorohydroquinone
substrate inhibition
Pseudomonas fluorescens
hydrogen peroxide
inactivation
Pseudomonas fluorescens
Hydroxyhydroquinone
0.2 mM, 9% residual activity
Pseudomonas fluorescens
methoxyhydroquinone
strong substrtae inhibition
Pseudomonas fluorescens
additional information
weak or no inhibition: 2-hydroxy-, 3-hydroxy-, 2,3-dihydroxy-, 2,5-dihydroxy-, 2,6-dihydroxy-, 3,4-dihydroxy-, 3,4,5-trihydroxy-, 3-chloro-4-hydroxy-, tetrafluoro-4-hydroxy-, 3-amino-4-hydroxy-, 4-hydroxy-3-methoxy-, 4-amino- and methyl 4-hydroxybenzoate; 6-hydroxynicotinate, 4-hydroxypropiophenone, 4-hydroxymandelate, 4-hydroxyphenylglycine, 4-hydroxybenzenesulfonic acid, and 4-methyl-, 4-methoxy-, and 4-aminophenol
Pseudomonas fluorescens
o-phenanthroline
inactivation
Pseudomonas fluorescens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.014
-
4-hydroxybenzoate
pH 7.0, 25°C
Pseudomonas fluorescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
hydroquinone
pH 7.0, 25°C
Pseudomonas fluorescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
10fold activation by Fe2+, each subunit binds one molecule of 4-hydroxybenzoate and one molecule of iron
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17800
-
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
38300
-
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
112000
-
gel filtration
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
Commentary
Organism
ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.89
-
pH 7.0, 25°C
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,3-difluorohydroquinone + O2
80% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
2,3-difluorohydroquinone + O2
80% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
2,5-difluorohydroquinone + O2
75% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
2,5-difluorohydroquinone + O2
75% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
3,5-difluorohydroquinone + O2
90% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
3,5-difluorohydroquinone + O2
90% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
bromohydroquinone + O2
30% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
bromohydroquinone + O2
30% of the activity with hydroquinone
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
chlorohydroquinone + O2
70% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
hydroquinone + O2
-
719714
Pseudomonas fluorescens
4-hydroxymuconic semialdehyde
-
-
-
?
methoxyhydroquinone + O2
50% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
methylhydroquinone + O2
120% of the activity with hydroquinone
719714
Pseudomonas fluorescens
?
-
-
-
?
additional information
no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol
719714
Pseudomonas fluorescens
?
-
-
-
?
additional information
no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol
719714
Pseudomonas fluorescens ACB
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit
Pseudomonas fluorescens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1
-
hydroquinone
pH 7.0, 25°C
Pseudomonas fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Pseudomonas fluorescens
Other publictions for EC 1.13.11.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744430
Ferraroni
The crystal structures of nat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Biochim. Biophys. Acta
1865
520-530
2017
-
-
-
1
-
-
2
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
6
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
745331
Liu
Crystal structure of PnpCD, a ...
Pseudomonas sp.
J. Biol. Chem.
290
24547-24560
2015
-
-
1
1
9
-
-
-
-
3
-
2
-
1
-
-
1
-
-
-
-
-
2
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
9
-
-
-
-
-
-
3
-
2
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
745802
Mancini
A copper-induced quinone degr ...
Lactococcus lactis, Lactococcus lactis IL1403
Mol. Microbiol.
95
645-659
2015
-
-
1
-
-
1
1
1
-
2
-
6
-
13
-
-
1
-
-
-
-
-
11
1
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
1
-
2
-
6
-
-
-
1
-
-
-
-
11
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp.
BMC Microbiol.
12
27
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
2
-
2
1
1
1
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
1
1
1
-
1
1
2
-
-
-
-
-
-
-
718555
Kolvenbach
Purification and characterizat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
AMB Express
1
08
2011
-
-
-
-
-
-
9
2
-
1
6
-
-
9
-
-
2
-
-
-
2
-
32
2
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
2
-
1
6
-
-
-
-
2
-
-
2
-
32
2
-
-
-
2
-
2
-
-
-
-
-
-
2
2
718916
Machonkin
Substrate specificity of Sphin ...
Sphingobium chlorophenolicum
Biochemistry
50
8899-8913
2011
-
-
-
-
-
-
3
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
12
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
719104
Shen
Cloning and characterization o ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Biores. Technol.
101
7516-7522
2010
-
-
-
-
2
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
-
-
4
2
2
-
-
-
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-
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-
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-
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-
2
-
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2
-
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-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719327
Yin
Characterization of MnpC, a hy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Curr. Microbiol.
61
471-476
2010
-
-
1
-
-
-
-
1
-
2
1
-
-
28
-
-
-
-
-
-
1
3
4
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
3
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
720066
Machonkin
Determination of the active si ...
Sphingobium chlorophenolicum
J. Biol. Inorg. Chem.
15
291-301
2010
-
-
1
1
5
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719714
Moonen
Hydroquinone dioxygenase from ...
Pseudomonas fluorescens, Pseudomonas fluorescens ACB
J. Bacteriol.
190
5199-5209
2008
1
-
-
-
-
-
12
1
-
1
3
-
-
13
-
-
1
-
-
-
1
-
14
1
-
-
-
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
12
1
1
-
1
3
-
-
-
-
1
-
-
1
-
14
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
718727
Xun
Characterization of 2,6-dichlo ...
Sphingobium chlorophenolicum
Biochem. Biophys. Res. Commun.
266
322-325
1999
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
718831
Xu
Evidence that pcpA encodes 2,6 ...
Sphingobium chlorophenolicum
Biochemistry
38
7659-7669
1999
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719693
Miyauchi
Cloning and sequencing of a no ...
Sphingomonas paucimobilis
J. Bacteriol.
181
6712-6719
1999
-
-
1
-
3
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
2
-
3
1
1
-
-
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1
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3
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1
1
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2
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3
1
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