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0.38 - 20.8
2-Ketobutyrate
1.18 - 189
2-ketocaproate
15.5
2-Ketoglutarate
-
30°C, pH 5.5, wild-type enzyme
0.124 - 31
2-ketoisocaproate
5.5 - 18.6
2-ketoisovalerate
3.8 - 12
2-oxoisocaproate
3.5
2-oxoisovalerate
pH 7.0, 30°C, recombinant enzyme
0.09 - 0.1
3,4-dihydroxyphenylpyruvic acid
7.7 - 38
3-methyl-2-oxobutanoate
4.4 - 10
alpha-Ketobutyrate
0.8
D-lactate
-
pH 8.0, 30°C
19
DL-2-hydroxybutyrate
-
pH 8.0, 25°C
0.28 - 53
Hydroxypyruvate
0.65 - 1.39
nicotinamide cytosine dinucleotide
additional information
additional information
-
0.59
(R)-lactate
-
-
8.5
(R)-lactate
-
pH 8.0, 25°C
9.54
(R)-lactate
pH 6.5, 30°C, recombinant enzyme
87.9
(R)-lactate
-
pH 8, , 30°C
260
(R)-lactate
-
pH 11.0, 20°C, recombinant enzyme
0.38
2-Ketobutyrate
-
30°C, pH 5.5, mutant enzyme Y52L
3.5
2-Ketobutyrate
-
30°C, pH 5.5, mutant enzyme Y52V
8.1
2-Ketobutyrate
-
30°C, pH 5.5, wild-type enzyme
20.8
2-Ketobutyrate
-
30°C, pH 5.5, mutant enzyme Y52A
1.18
2-ketocaproate
-
30°C, pH 5.5, mutant enzyme Y52R
13.3
2-ketocaproate
-
30°C, pH 5.5, wild-type enzyme
14.1
2-ketocaproate
-
30°C, pH 5.5, mutant enzyme Y52R/F299G
51
2-ketocaproate
-
30°C, pH 5.5, mutant enzyme Y52V
102
2-ketocaproate
-
30°C, pH 5.5, mutant enzyme Y52A
189
2-ketocaproate
-
30°C, pH 5.5, mutant enzyme Y52L
0.124
2-ketoisocaproate
-
30°C, pH 5.5, mutant enzyme Y52L
0.7
2-ketoisocaproate
-
30°C, pH 5.5, mutant enzyme Y52V
12.1
2-ketoisocaproate
-
30°C, pH 5.5, mutant enzyme Y52A
31
2-ketoisocaproate
-
30°C, pH 5.5, wild-type enzyme
5.5
2-ketoisovalerate
-
30°C, pH 5.5, wild-type enzyme
18.6
2-ketoisovalerate
-
30°C, pH 5.5, mutant enzyme Y52L
0.15
2-ketovalerate
-
30°C, pH 5.5, mutant enzyme Y52L
0.8
2-ketovalerate
-
30°C, pH 5.5, mutant enzyme Y52V
11.7
2-ketovalerate
-
30°C, pH 5.5, mutant enzyme Y52A
17
2-ketovalerate
-
30°C, pH 5.5, wild-type enzyme
2.7
2-oxobutanoate
mutant Y51F, pH 5.5, 30°C
7.5
2-oxobutanoate
mutant Y51M, pH 5.5, 30°C
11
2-oxobutanoate
wild-type, pH 5.5, 30°C
12
2-oxobutanoate
mutant Y51L, pH 5.5, 30°C
234
2-oxobutanoate
-
pH 8, 30°C
0.31
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
0.64
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
2.3
2-oxobutyrate
-
pH 8.0, 25°C
8.1
2-oxobutyrate
-
30°C, pH 5.5, wild-type enzyme
30
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
58
2-oxobutyrate
-
30°C, pH 5.5, mutant enzyme N97D
2.6
2-oxocaproate
pH 7.0, 30°C, recombinant enzyme
5.8
2-oxocaproate
pH 7.0, 30°C, recombinant enzyme
3.8
2-oxoisocaproate
pH 7.0, 30°C, recombinant enzyme
12
2-oxoisocaproate
pH 7.0, 30°C, recombinant enzyme
0.68
2-oxovalerate
pH 7.0, 30°C, recombinant enzyme
4
2-oxovalerate
pH 7.0, 30°C, recombinant enzyme
0.09
3,4-dihydroxyphenylpyruvic acid
-
cosubstrate NADH, pH 8.0, 25°C
0.1
3,4-dihydroxyphenylpyruvic acid
-
cosubstrate NADPH, pH 8.0, 25°C
7.7
3-methyl-2-oxobutanoate
mutant Y51F, pH 5.5, 30°C
8.6
3-methyl-2-oxobutanoate
mutant Y51M, pH 5.5, 30°C
12
3-methyl-2-oxobutanoate
mutant Y51L, pH 5.5, 30°C
38
3-methyl-2-oxobutanoate
wild-type, pH 5.5, 30°C
4.4
alpha-Ketobutyrate
-
-
10
alpha-Ketobutyrate
-
-
5.4
glyoxylate
-
30°C, pH 5.5, wild-type enzyme
6.8
glyoxylate
pH 7.0, 30°C, recombinant enzyme
18
glyoxylate
pH 7.0, 30°C, recombinant enzyme
20
glyoxylate
pH 7.0, 30°C, recombinant enzyme
72
glyoxylate
-
30°C, pH 5.5, mutant enzyme N97D
0.28
Hydroxypyruvate
-
30°C, pH 5.5, wild-type enzyme
2.1
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme Y52R/F299G
3
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
3
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme Y52L
8.7
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme Y52V
12
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
17
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme Y52A
18
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
18.6
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme F299S
20.2
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme Y52R
29
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme N97D
53
Hydroxypyruvate
-
30°C, pH 5.5, mutant enzyme F299G
0.00044
NAD+
-
-
0.28
NAD+
wild-type, pH not specified in the publication, temperature not specified in the publication
0.41
NAD+
-
isozyme D-LDH3, pH 8.0, 25°C
0.87
NAD+
mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication
0.92
NAD+
mutant V152R, pH not specified in the publication, temperature not specified in the publication
1.05
NAD+
mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication
1.6
NAD+
mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication
1.96
NAD+
-
pH 11.0, 20°C, recombinant enzyme
2.58
NAD+
-
isozyme D-LDH2, pH 8.0, 25°C
2.75
NAD+
-
isozyme D-LDH1, pH 8.0, 25°C
0.00021
NADH
-
-
0.019
NADH
pH 7.0, 30°C, recombinant enzyme
0.022
NADH
pH 7.0, 30°C, recombinant enzyme
0.028
NADH
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
0.031
NADH
pH 8.0, 30°C, recombinant enzyme, with Mg2+
0.035
NADH
pH 8.0, 30°C, recombinant enzyme, with or without fructose 1,6-bisphosphate and Mg2+
0.036
NADH
pH 8.0, 30°C, recombinant enzyme
0.04
NADH
-
isozyme D-LDH3, pH 8.0, 25°C
0.055
NADH
pH 7.0, 30°C, recombinant enzyme
0.088
NADH
pH 8.0, 30°C, recombinant enzyme
0.09
NADH
pH 8.0, 30°C, recombinant enzyme, with Mg2+
0.092
NADH
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
0.5
NADH
-
pH 8.0, 30°C, recombinant enzyme
0.7
NADH
-
isozyme D-LDH1, pH 8.0, 25°C
2.83
NADH
-
isozyme D-LDH2, pH 8.0, 25°C
0.65
nicotinamide cytosine dinucleotide
mutant V152R, pH not specified in the publication, temperature not specified in the publication
0.66
nicotinamide cytosine dinucleotide
mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication
1.38
nicotinamide cytosine dinucleotide
mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication
1.38
nicotinamide cytosine dinucleotide
mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication
1.39
nicotinamide cytosine dinucleotide
wild-type, pH not specified in the publication, temperature not specified in the publication
1.7
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
2.2
oxaloacetate
-
30°C, pH 5.5, wild-type enzyme
2.5
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
3.8
oxaloacetate
-
30°C, pH 5.5, mutant enzyme Y52R
7.5 - 9
oxaloacetate
-
recombinant and wild-type enzyme
43.1
oxaloacetate
-
30°C, pH 5.5, mutant enzyme F299S
54
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
78.8
oxaloacetate
-
pH 8, 30°C
0.03
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52A
0.06
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52L
0.14
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52V
0.16
phenylpyruvate
mutant Y51A, pH 5.5, 30°C
0.17
phenylpyruvate
mutant Y51L, pH 5.5, 30°C
0.22
phenylpyruvate
mutant Y51F, pH 5.5, 30°C
0.22
phenylpyruvate
mutant Y51M, pH 5.5, 30°C
0.27
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant Y52L
0.32
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant F299Y
0.4
phenylpyruvate
mutant Y51S, pH 5.5, 30°C
0.49
phenylpyruvate
-
pH 5.5, 45°C
0.8
phenylpyruvate
-
30°C, pH 5.5, wild-type enzyme
1.4
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant Y52L/F299Y
1.68
phenylpyruvate
-
pH 8, 50°C
1.73
phenylpyruvate
-
pH 5.5, 45°C
2.1
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52R
2.6
phenylpyruvate
wild-type, pH 5.5, 30°C
2.83
phenylpyruvate
pH 7, 30°C
2.9
phenylpyruvate
-
recombinant enzyme, pH 5.5, 30°C
3.32
phenylpyruvate
recombinant enzyme, pH and temperature not specified in the publication
6.9
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52R/F299G
7.1
phenylpyruvate
pH 7.0, 30°C, recombinant enzyme
7.2
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme F299S
8.7
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme Y52T/D299S
11
phenylpyruvate
pH 7.0, 30°C, recombinant enzyme
11.4
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged wild-type enzyme
22
phenylpyruvate
-
30°C, pH 5.5, mutant enzyme F299G
0.07
pyruvate
-
-
0.09
pyruvate
-
recombinant enzyme, pH 5.5, 30°C
0.1
pyruvate
pH 7.0, 30°C, recombinant enzyme
0.12
pyruvate
-
30°C, pH 5.5, wild-type enzyme
0.3
pyruvate
pH 6.5, 30°C, recombinant enzyme
0.34
pyruvate
pH 7.0, 30°C, recombinant enzyme
0.36
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
0.47
pyruvate
-
isoform LDHD1, pH 6, 35°C
0.58
pyruvate
-
pH 8.0, 30°C, recombinant enzyme
0.67
pyruvate
-
pH 8.0, 25°C
0.85
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
1.2
pyruvate
pH 8.0, 30°C, recombinant enzyme
1.2
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
1.4
pyruvate
-
isoform LDHD2, pH 7.5, 35°C
1.5 - 1.6
pyruvate
-
recombinant and wild-type enzyme
1.7
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
1.8
pyruvate
-
30°C, pH 5.5, mutant enzyme Y52L
2.5
pyruvate
pH 8.0, 30°C, recombinant enzyme
2.6
pyruvate
pH 7.0, 30°C, recombinant enzyme
2.66
pyruvate
-
pH 8, 30°C
2.7
pyruvate
pH 6.5, 30°C, recombinant enzyme
3.1
pyruvate
-
isoform LDHD3, pH 5.5, 35°C
3.4
pyruvate
recombinant enzyme, pH and temperature not specified in the publication
5.9
pyruvate
pH 6.5, 30°C, recombinant enzyme
6
pyruvate
-
mutant A234C, pH 7, 27°C
6.1
pyruvate
-
mutant G297A, pH 7, 27°C
6.5
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
7.2
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
7.6
pyruvate
pH 8.0, 30°C, recombinant enzyme
8.2
pyruvate
-
30°C, pH 5.5, mutant enzyme N97D
9.2
pyruvate
-
mutant A78V, pH 7, 27°C
9.5
pyruvate
-
mutant A234S, pH 7, 27°C
10.6
pyruvate
-
mutant T75L/A234S, pH 7, 27°C
11.5
pyruvate
-
mutant T75L/A234G, pH 7, 27°C
12.6
pyruvate
-
30°C, pH 5.5, mutant enzyme Y52A
13.1
pyruvate
-
30°C, pH 5.5, mutant enzyme Y52V
14.1
pyruvate
-
mutant T75L, pH 7, 27°C
14.8
pyruvate
-
wild-type, pH 7, 27°C
15.3
pyruvate
-
mutant A234T, pH 7, 27°C
18.5
pyruvate
-
mutant A234G, pH 7, 27°C
20.8
pyruvate
-
mutant F299Y, pH 7, 27°C
21.4
pyruvate
-
mutant T260V, pH 7, 27°C
31
pyruvate
-
30°C, pH 5.5, mutant enzyme F299G
67.3
pyruvate
-
mutant A100V, pH 7, 27°C
136
pyruvate
-
mutant N263G, pH 7, 27°C
additional information
additional information
kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5
-
additional information
additional information
-
kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows negative cooperativity in the pyruvate saturation curve above pH 7.5
-
additional information
additional information
kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5
-
additional information
additional information
kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5
-
additional information
additional information
-
kinetics and allosteric mechanism of D-LDHs, overview. The enzyme shows a hyperbolic shaped pyruvate saturation curve below pH 6.5, and shows positive cooperativity in the pyruvate saturation curve above pH 7.5
-