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Literature summary for 1.1.1.28 extracted from

  • Luo, X.; Zhang, Y.; Yin, F.; Hu, G.; Jia, Q.; Yao, C.; Fu, Y.
    Enzymological characterization of a novel D-lactate dehydrogenase from Lactobacillus rossiae and its application in D-phenyllactic acid synthesis (2020), 3 Biotech, 10, 101 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.28

Application

Application Comment Organism
synthesis recombinant Escherichia coli expressing D-lactate dehydrogenase, without coexpression of a cofactor regeneration system, can produce 20.5 g/l D-phenyllactic acid with enantiomeric excess above 99% from phenylpyruvic acid in a fed-batch biotransformation process, with a productivity of 49.2 g/l per day Furfurilactobacillus rossiae

General Stability

General Stability Organism
stable in a neutral to alkaline environment below 35 °C Furfurilactobacillus rossiae

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 13% residual activity Furfurilactobacillus rossiae
Ba2+ 1 mM, 88% residual activity Furfurilactobacillus rossiae
Cu2+ 1 mM, 83% residual activity Furfurilactobacillus rossiae
Fe2+ 1 mM, 66% residual activity Furfurilactobacillus rossiae
Fe3+ 1 mM, 73% residual activity Furfurilactobacillus rossiae
Mg2+ 1 mM, 85% residual activity Furfurilactobacillus rossiae
Mn2+ 1 mM, 97% residual activity Furfurilactobacillus rossiae
additional information not inhibitory: EDTA at 2%, Ca2+ Furfurilactobacillus rossiae
Ni2+ 1 mM, 80% residual activity Furfurilactobacillus rossiae
SDS 1%, 9% residual activity Furfurilactobacillus rossiae
Triton X-100 1%, 81% residual activity Furfurilactobacillus rossiae
Tween 80 1%, 89% residual activity Furfurilactobacillus rossiae
Zn2+ 1 mM, 114% residual activity Furfurilactobacillus rossiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.83
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1 mM, 109% of initial activity Furfurilactobacillus rossiae
Co2+ 1 mM, 115% of initial activity Furfurilactobacillus rossiae

Organic Solvent Stability

Organic Solvent Comment Organism
butyl acetate 2%, 127% of initial activity Furfurilactobacillus rossiae
cyclohexane 2%, 146% of initial activity Furfurilactobacillus rossiae
dibutyl phthalate 2%, 90% of initial activity Furfurilactobacillus rossiae
dichloromethane 2%, 84% of initial activity Furfurilactobacillus rossiae
dimethyl sulfoxide 2%, 88% of initial activity Furfurilactobacillus rossiae
Ethanol 2%, 73% of initial activity Furfurilactobacillus rossiae
Ethyl acetate 2%, 114% of initial activity Furfurilactobacillus rossiae
Glycerol 2%, 92% of initial activity Furfurilactobacillus rossiae
isobutanol 2%, 91% of initial activity Furfurilactobacillus rossiae
isopropanol 2%, 93% of initial activity Furfurilactobacillus rossiae
Methanol 2%, 94% of initial activity Furfurilactobacillus rossiae
n-hexane 2%, 80% of initial activity Furfurilactobacillus rossiae

Organism

Organism UniProt Comment Textmining
Furfurilactobacillus rossiae A0A0R1RJ30
-
-
Furfurilactobacillus rossiae DSM 15814 A0A0R1RJ30
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylpyruvate + NADH + H+
-
 Furfurilactobacillus rossiae D-phenyllactate + NAD+
-
 ?
phenylpyruvate + NADH + H+
-
 Furfurilactobacillus rossiae DSM 15814 D-phenyllactate + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 38700, calculated from sequence Furfurilactobacillus rossiae

Synonyms

Synonyms Comment Organism
FD35_GL001981
-
 Furfurilactobacillus rossiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Furfurilactobacillus rossiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 half-life 1453 min Furfurilactobacillus rossiae
40
-
 half-life 11 min Furfurilactobacillus rossiae
50
-
 half-life 3.7 min Furfurilactobacillus rossiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.29
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Furfurilactobacillus rossiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 half-life 895 min Furfurilactobacillus rossiae
7
-
 half-life 1655 min Furfurilactobacillus rossiae
8
-
 half-life 2319 min Furfurilactobacillus rossiae
9
-
 half-life 1315 min Furfurilactobacillus rossiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Furfurilactobacillus rossiae
NADPH NADH is preferred over NADPH Furfurilactobacillus rossiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.34
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae