Literature summary for 1.1.1.28 extracted from

Liu, Y.; Li, Q.; Wang, L.; Guo, X.; Wang, J.; Wang, Q.; Zhao, Z.K.
Engineering D-lactate dehydrogenase to favor an non-natural cofactor nicotinamide cytosine dinucleotide (2020), ChemBioChem, 21, 1972-1975 .

Search for more literature for 1.1.1.28

Protein Variants

Protein Variants	Comment	Organism
V152R	catalytic efficiency with NAD analogue nicotinamide cytosine dinucleotide is 2.4fold higher than that with NAD	Lactobacillus helveticus
V152R/I177K	mutant shows good activity with NAD analogue nicotinamide cytosine dinucleotide and reduced activity with NAD	Lactobacillus helveticus
V152R/I177K/N213I	triple mutant shows high preference or activity with NAD analogue nicotinamide cytosine dinucleotide	Lactobacillus helveticus
V152R/N213E	mutant shows good activity with NAD analogue nicotinamide cytosine dinucleotide and reduced activity with NAD	Lactobacillus helveticus
V152R/V210N/N213E	triple mutant shows high preference or activity with NAD analogue nicotinamide cytosine dinucleotide	Lactobacillus helveticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.28	-	NAD+	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.65	-	nicotinamide cytosine dinucleotide	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.66	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.87	-	NAD+	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.92	-	NAD+	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
1.05	-	NAD+	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
1.38	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
1.38	-	nicotinamide cytosine dinucleotide	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
1.39	-	nicotinamide cytosine dinucleotide	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
1.6	-	NAD+	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus helveticus	P30901	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-lactate + NAD+ + H+	-	Lactobacillus helveticus	pyruvate + NADH	-	?
D-lactate + nicotinamide cytosine dinucleotide	-	Lactobacillus helveticus	pyruvate + reduced nicotinamide cytosine dinucleotide	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.08	-	NAD+	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.08	-	NAD+	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.62	-	NAD+	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.66	-	nicotinamide cytosine dinucleotide	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.02	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.14	-	NAD+	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.84	-	NAD+	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.95	-	nicotinamide cytosine dinucleotide	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
3.61	-	nicotinamide cytosine dinucleotide	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
4.51	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus

General Information

General Information	Comment	Organism
metabolism	amino acid residues I177 and N213 form a gate guarding the NAD adenine moiety binding cavity	Lactobacillus helveticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.05	-	NAD+	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.074	-	NAD+	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.48	-	nicotinamide cytosine dinucleotide	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
0.71	-	NAD+	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.1	-	nicotinamide cytosine dinucleotide	mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
2.3	-	NAD+	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
3.1	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
3.3	-	nicotinamide cytosine dinucleotide	mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
5.6	-	nicotinamide cytosine dinucleotide	mutant V152R, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus
10.1	-	NAD+	wild-type, pH not specified in the publication, temperature not specified in the publication	Lactobacillus helveticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)