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Literature summary for 1.1.1.28 extracted from
- Major role of NAD-dependent lactate dehydrogenases in the production of L-lactic acid with high optical purity by the thermophile Bacillus coagulans (2014), Appl. Environ. Microbiol., 80, 7134-7141.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ldhD, quantitative enzyme realtime PCR expression analysis during ferentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactobacillus delbrueckii subsp. bulgaricus |
| gene ldhD, quantitative enzyme realtime PCR expression analysis during fermentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Weizmannia coagulans |
| gene ldhD, quantitative enzyme realtime PCR expression analysis during fermentation, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactiplantibacillus plantarum subsp. plantarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus |  |
| 2.7 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
| 5.9 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
| 9.54 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Weizmannia coagulans | the enzyme shows very low in vivo activity | ? | - |
? | |
| additional information | Weizmannia coagulans 2-6 | the enzyme shows very low in vivo activity | ? | - |
? | |
| pyruvate + NADH + H+ | Lactobacillus delbrueckii subsp. bulgaricus | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Weizmannia coagulans | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Lactiplantibacillus plantarum subsp. plantarum | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | - |
(R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | Weizmannia coagulans 2-6 | - |
(R)-lactate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum subsp. plantarum | C0LJH4 | - |
- |
| Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | C0LJH4 | - |
- |
| Lactobacillus delbrueckii subsp. bulgaricus | P26297 | - |
- |
| Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | P26297 | - |
- |
| Weizmannia coagulans | F8RPR8 | - |
- |
| Weizmannia coagulans 2-6 | F8RPR8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus delbrueckii subsp. bulgaricus |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Weizmannia coagulans |
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactiplantibacillus plantarum subsp. plantarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain 2-6 is an L-lactic acid producer, it is a homofermentative producer of L-lactic acid with an optical purity of 99% | Weizmannia coagulans | - |
| additional information | strain DSM 20081 is a D-lactic acid producer producing D-lactic acid at an optical purity of 98% | Lactobacillus delbrueckii subsp. bulgaricus | - |
| additional information | strain DSM 20174 is a DL-lactic acid producer, 1:1 ratio of L-lactic acid to D-lactic acid | Lactiplantibacillus plantarum subsp. plantarum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
crude enzyme, pyruvate reduction in vivo activity is below detection limit | Weizmannia coagulans |
| 2.51 | - |
crude enzyme, pyruvate reduction in vivo activity, pH 6.5, 30°C | Lactiplantibacillus plantarum subsp. plantarum |
| 8.3 | - |
crude enzyme, pyruvate reduction in vivo activity, pH 6.5, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows very low in vivo activity | Weizmannia coagulans | ? | - |
? | |
| additional information | the enzyme shows very low in vivo activity | Weizmannia coagulans 2-6 | ? | - |
? | |
| pyruvate + NADH + H+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Weizmannia coagulans | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Lactiplantibacillus plantarum subsp. plantarum | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Lactiplantibacillus plantarum subsp. plantarum DSM 20174 | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Lactobacillus delbrueckii subsp. bulgaricus DSM 20081 | (R)-lactate + NAD+ | - |
r | |
| pyruvate + NADH + H+ | - |
Weizmannia coagulans 2-6 | (R)-lactate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-nLDH | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| D-nLDH | - |
Weizmannia coagulans |
| D-nLDH | - |
Lactiplantibacillus plantarum subsp. plantarum |
| ldhd | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| ldhd | - |
Weizmannia coagulans |
| ldhd | - |
Lactiplantibacillus plantarum subsp. plantarum |
| NAD-dependent D-lactate dehydrogenase | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| NAD-dependent D-lactate dehydrogenase | - |
Weizmannia coagulans |
| NAD-dependent D-lactate dehydrogenase | - |
Lactiplantibacillus plantarum subsp. plantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lactiplantibacillus plantarum subsp. plantarum |
| 35 | - |
- |
Lactobacillus delbrueckii subsp. bulgaricus |
| 35 | - |
- |
Weizmannia coagulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
| 11.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
| 91.3 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
| 235.5 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Lactobacillus delbrueckii subsp. bulgaricus |
| 6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Weizmannia coagulans |
| 6.5 | - |
recombinant enzyme, pyruvate reduction assay at | Lactiplantibacillus plantarum subsp. plantarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
| NAD+ | - |
Weizmannia coagulans | |
| NAD+ | - |
Lactiplantibacillus plantarum subsp. plantarum | |
| NADH | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
| NADH | - |
Weizmannia coagulans | |
| NADH | - |
Lactiplantibacillus plantarum subsp. plantarum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | ldhD transcription levels are higher than those of ldhL, EC 1.1.1.27 | Lactobacillus delbrueckii subsp. bulgaricus |
| additional information | ldhD transcription levels are higher than those of ldhL, EC 1.1.1.27 | Lactiplantibacillus plantarum subsp. plantarum |
| additional information | ldhL, EC 1.1.1.27, transcription levels are higher than those of ldhD | Weizmannia coagulans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0073 | - |
(R)-lactate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
| 1.9 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Weizmannia coagulans | |
| 34 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactiplantibacillus plantarum subsp. plantarum | |
| 7900 | - |
pyruvate | pH 6.5, 30°C, recombinant enzyme | Lactobacillus delbrueckii subsp. bulgaricus | |
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Release 2023.1 (January 2023)
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