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Literature summary for 1.1.1.28 extracted from

  • Nakano, K.; Sawada, S.; Yamada, R.; Mimitsuka, T.; Ogino, H.
    Enhancement of the catalytic activity of D-lactate dehydrogenase from Sporolactobacillus laevolacticus by site-directed mutagenesis (2018), Biochem. Eng. J., 133, 214-218 .
No PubMed abstract available
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sporolactobacillus laevolacticus

Protein Variants

Protein Variants Comment Organism
A100V less than 10% of wild-type catalytic efficiency Sporolactobacillus laevolacticus
A234C about 30% of wild-type catalytic efficiency Sporolactobacillus laevolacticus
A234G mutation enhances catalytic activity toward pyruvate Sporolactobacillus laevolacticus
A234S mutation enhances catalytic activity toward pyruvate Sporolactobacillus laevolacticus
A234T about 15% of wild-type catalytic efficiency Sporolactobacillus laevolacticus
A78V specific activity similar to wild-type Sporolactobacillus laevolacticus
F299Y specific activity similar to wild-type Sporolactobacillus laevolacticus
G297A about 20% increase in activity Sporolactobacillus laevolacticus
N263G about 30% of wild-type activity Sporolactobacillus laevolacticus
T260V specific activity similar to wild-type Sporolactobacillus laevolacticus
T75L increase in specific acitivity Sporolactobacillus laevolacticus
T75L/A234g mutation improves kcat/Km by 5fold Sporolactobacillus laevolacticus
T75L/A234S mutation improves kcat/Km by 6.8fold Sporolactobacillus laevolacticus
T77N insoluble Sporolactobacillus laevolacticus
V296G strong decrease in activity Sporolactobacillus laevolacticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 pyruvate mutant A234C, pH 7, 27°C Sporolactobacillus laevolacticus
6.1
-
 pyruvate mutant G297A, pH 7, 27°C Sporolactobacillus laevolacticus
9.2
-
 pyruvate mutant A78V, pH 7, 27°C Sporolactobacillus laevolacticus
9.5
-
 pyruvate mutant A234S, pH 7, 27°C Sporolactobacillus laevolacticus
10.6
-
 pyruvate mutant T75L/A234S, pH 7, 27°C Sporolactobacillus laevolacticus
11.5
-
 pyruvate mutant T75L/A234G, pH 7, 27°C Sporolactobacillus laevolacticus
14.1
-
 pyruvate mutant T75L, pH 7, 27°C Sporolactobacillus laevolacticus
14.8
-
 pyruvate wild-type, pH 7, 27°C Sporolactobacillus laevolacticus
15.3
-
 pyruvate mutant A234T, pH 7, 27°C Sporolactobacillus laevolacticus
18.5
-
 pyruvate mutant A234G, pH 7, 27°C Sporolactobacillus laevolacticus
20.8
-
 pyruvate mutant F299Y, pH 7, 27°C Sporolactobacillus laevolacticus
21.4
-
 pyruvate mutant T260V, pH 7, 27°C Sporolactobacillus laevolacticus
67.3
-
 pyruvate mutant A100V, pH 7, 27°C Sporolactobacillus laevolacticus
136
-
 pyruvate mutant N263G, pH 7, 27°C Sporolactobacillus laevolacticus

Organism

Organism UniProt Comment Textmining
Sporolactobacillus laevolacticus
-
-
-
Sporolactobacillus laevolacticus JCM 2513
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
 reduction of pyruvate, pH 7, 27°C Sporolactobacillus laevolacticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+
-
 Sporolactobacillus laevolacticus (R)-lactate + NAD+
-
 ?
pyruvate + NADH + H+
-
 Sporolactobacillus laevolacticus JCM 2513 (R)-lactate + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE and calculated from sequence Sporolactobacillus laevolacticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00008
-
 pyruvate mutant A234C, pH 7, 27°C Sporolactobacillus laevolacticus
0.00009
-
 pyruvate mutant A234T, pH 7, 27°C Sporolactobacillus laevolacticus
0.00016
-
 pyruvate mutant A100V, pH 7, 27°C Sporolactobacillus laevolacticus
0.00019
-
 pyruvate mutant G297A, pH 7, 27°C Sporolactobacillus laevolacticus
0.00031
-
 pyruvate mutant A78V, pH 7, 27°C Sporolactobacillus laevolacticus
0.00044
-
 pyruvate mutant F299Y, pH 7, 27°C Sporolactobacillus laevolacticus
0.00046
-
 pyruvate wild-type, pH 7, 27°C Sporolactobacillus laevolacticus
0.00057
-
 pyruvate mutant A234S, pH 7, 27°C Sporolactobacillus laevolacticus
0.00057
-
 pyruvate mutant T260V, pH 7, 27°C Sporolactobacillus laevolacticus
0.00069
-
 pyruvate mutant A234G, pH 7, 27°C Sporolactobacillus laevolacticus
0.00072
-
 pyruvate mutant N263G, pH 7, 27°C Sporolactobacillus laevolacticus
0.00078
-
 pyruvate mutant T75L, pH 7, 27°C Sporolactobacillus laevolacticus
0.00181
-
 pyruvate mutant T75L/A234G, pH 7, 27°C Sporolactobacillus laevolacticus
0.00225
-
 pyruvate mutant T75L/A234S, pH 7, 27°C Sporolactobacillus laevolacticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000002
-
 pyruvate mutant A100V, pH 7, 27°C Sporolactobacillus laevolacticus
0.000005
-
 pyruvate mutant N263G, pH 7, 27°C Sporolactobacillus laevolacticus
0.000006
-
 pyruvate mutant A234T, pH 7, 27°C Sporolactobacillus laevolacticus
0.000013
-
 pyruvate mutant A234C, pH 7, 27°C Sporolactobacillus laevolacticus
0.000021
-
 pyruvate mutant F299Y, pH 7, 27°C Sporolactobacillus laevolacticus
0.000027
-
 pyruvate mutant T260V, pH 7, 27°C Sporolactobacillus laevolacticus
0.000031
-
 pyruvate mutant G297A, pH 7, 27°C Sporolactobacillus laevolacticus
0.000031
-
 pyruvate wild-type, pH 7, 27°C Sporolactobacillus laevolacticus
0.000034
-
 pyruvate mutant A78V, pH 7, 27°C Sporolactobacillus laevolacticus
0.000037
-
 pyruvate mutant A234G, pH 7, 27°C Sporolactobacillus laevolacticus
0.000055
-
 pyruvate mutant T75L, pH 7, 27°C Sporolactobacillus laevolacticus
0.00006
-
 pyruvate mutant A234S, pH 7, 27°C Sporolactobacillus laevolacticus
0.000157
-
 pyruvate mutant T75L/A234G, pH 7, 27°C Sporolactobacillus laevolacticus
0.000211
-
 pyruvate mutant T75L/A234S, pH 7, 27°C Sporolactobacillus laevolacticus