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Literature summary for 1.1.1.28 extracted from

  • Lee, H.S.; Park, J.; Yoo, Y.J.; Yeon, Y.J.
    Engineering D-lactate dehydrogenase from Pediococcus acidilactici for improved ctivity on 2-hydroxy acids with bulky C3 functional group (2019), Appl. Biochem. Biotechnol., 189, 1141-1155 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Pediococcus acidilactici

Protein Variants

Protein Variants Comment Organism
F298L negligible activity Pediococcus acidilactici
F298V negligible activity Pediococcus acidilactici
N76A reduced activity compared to wild-type Pediococcus acidilactici
N76I negligible activity Pediococcus acidilactici
N76L reduced activity compared to wild-type Pediococcus acidilactici
N76V reduced activity compared to wild-type Pediococcus acidilactici
V77I reduced activity compared to wild-type Pediococcus acidilactici
Y100F reduced activity compared to wild-type Pediococcus acidilactici
Y100I negligible activity Pediococcus acidilactici
Y100L negligible activity Pediococcus acidilactici
Y51A mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici
Y51F mutant with improved catalytic efficiency on 2-oxobutanoate and 3-methyl-2-oxobutanoate of 37.2 and 23.2 per s and mM, respectively Pediococcus acidilactici
Y51L mutant with improved catalytic efficiency on phenylpyruvate of 2200 per s and mM Pediococcus acidilactici
Y51M mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici
Y51S mutant with improved catalytic efficiency on phenylpyruvate Pediococcus acidilactici

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
0.17
-
phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
0.22
-
phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
0.22
-
phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
0.4
-
phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
2.6
-
phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
2.7
-
2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
7.5
-
2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
7.7
-
3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
8.6
-
3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
11
-
2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
12
-
2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
12
-
3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
38
-
3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici

Organism

Organism UniProt Comment Textmining
Pediococcus acidilactici E0NDE9
-
-
Pediococcus acidilactici DSM 20284 E0NDE9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NADH + H+
-
Pediococcus acidilactici 2-hydroxybutanoate + NAD+
-
?
2-oxobutanoate + NADH + H+
-
Pediococcus acidilactici DSM 20284 2-hydroxybutanoate + NAD+
-
?
3-methyl-2-oxobutanoate + NADH + H+
-
Pediococcus acidilactici 2-hydroxy-3-methylbutanoate + NAD+
-
?
3-methyl-2-oxobutanoate + NADH + H+
-
Pediococcus acidilactici DSM 20284 2-hydroxy-3-methylbutanoate + NAD+
-
?
phenylpyruvate + NADH + H+
-
Pediococcus acidilactici (R)-3-phenyllactate + NAD+
-
?
phenylpyruvate + NADH + H+
-
Pediococcus acidilactici DSM 20284 (R)-3-phenyllactate + NAD+
-
?

Synonyms

Synonyms Comment Organism
D-LDH
-
Pediococcus acidilactici
ldhd
-
Pediococcus acidilactici

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.9
-
3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
41.3
-
phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
50.7
-
2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
76.2
-
3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
90.2
-
3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
99.4
-
2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
152
-
2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
179
-
3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
199
-
2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
239
-
phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
269
-
phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
376
-
phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
391
-
phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
462
-
phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.89
-
3-methyl-2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
4.4
-
2-oxobutanoate wild-type, pH 5.5, 30°C Pediococcus acidilactici
6 12 phenylpyruvate mutant Y51S, pH 5.5, 30°C Pediococcus acidilactici
7.4
-
3-methyl-2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
8.9
-
3-methyl-2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
15.9
-
phenylpyruvate wild-type, pH 5.5, 30°C Pediococcus acidilactici
16.8
-
2-oxobutanoate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici
20.7
-
2-oxobutanoate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
23.2
-
3-methyl-2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
37.2
-
2-oxobutanoate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
1720
-
phenylpyruvate mutant Y51A, pH 5.5, 30°C Pediococcus acidilactici
1880
-
phenylpyruvate mutant Y51F, pH 5.5, 30°C Pediococcus acidilactici
2130
-
phenylpyruvate mutant Y51M, pH 5.5, 30°C Pediococcus acidilactici
2200
-
phenylpyruvate mutant Y51L, pH 5.5, 30°C Pediococcus acidilactici