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Literature summary for 1.1.1.28 extracted from

  • Yu, S.; Jiang, H.; Jiang, B.; Mu, W.
    Characterization of D-lactate dehydrogenase producing D-3-phenyllactic acid from Pediococcus pentosaceus (2012), Biosci. Biotechnol. Biochem., 76, 853-855.
    View publication on PubMed
Search for more literature for 1.1.1.28

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ slight inhibition at 1 mM Pediococcus pentosaceus
Cu2+ slight inhibition at 1 mM Pediococcus pentosaceus
Mn2+ slight inhibition at 1 mM Pediococcus pentosaceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
1.73
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates by 15% at 1 mM Pediococcus pentosaceus
Mg2+ very slightly activating Pediococcus pentosaceus
Ni2+ activates by 35% at 1 mM Pediococcus pentosaceus
Zn2+ very slightly activating Pediococcus pentosaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37203
-
 x * 37203, sequence calculation Pediococcus pentosaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Pediococcus pentosaceus
-
 pyruvate + NADH + H+
-
 r
(R)-lactate + NAD+ Pediococcus pentosaceus ATCC 25745
-
 pyruvate + NADH + H+
-
 r
phenylpyruvate + NADH + H+ Pediococcus pentosaceus
-
 (R)-3-phenyllactate + NAD+
-
 ?
phenylpyruvate + NADH + H+ Pediococcus pentosaceus ATCC 25745
-
 (R)-3-phenyllactate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Pediococcus pentosaceus
-
-
-
Pediococcus pentosaceus ATCC 25745
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
116
-
 substrate phenylpyruvate, pH 5.5, 45°C Pediococcus pentosaceus
835
-
 substrate pyruvate, pH 5.5, 45°C Pediococcus pentosaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Pediococcus pentosaceus pyruvate + NADH + H+
-
 r
(R)-lactate + NAD+
-
 Pediococcus pentosaceus ATCC 25745 pyruvate + NADH + H+
-
 r
phenylpyruvate + NADH + H+
-
 Pediococcus pentosaceus (R)-3-phenyllactate + NAD+
-
 ?
phenylpyruvate + NADH + H+
-
 Pediococcus pentosaceus ATCC 25745 (R)-3-phenyllactate + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 37203, sequence calculation Pediococcus pentosaceus

Synonyms

Synonyms Comment Organism
D-LDH
-
 Pediococcus pentosaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pediococcus pentosaceus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range, profile overview Pediococcus pentosaceus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 45 87% activity remaining after 4 h at 30°C, higher loss of activity above 45°C Pediococcus pentosaceus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
173
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
320
-
 pyruvate pH 5.5, 45°C Pediococcus pentosaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pediococcus pentosaceus

pH Range

pH Minimum pH Maximum Comment Organism
3 9 activity range, profile overview Pediococcus pentosaceus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pediococcus pentosaceus
NADH
-
 Pediococcus pentosaceus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
 phenylpyruvate pH 5.5, 45°C Pediococcus pentosaceus
658
-
 pyruvate pH 5.5, 45°C Pediococcus pentosaceus