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Literature summary for 1.1.1.28 extracted from

  • Chen, L.; Bai, Y.; Fan, T.P.; Zheng, X.; Cai, Y.
    Characterization of a D-lactate dehydrogenase from Lactobacillus fermentum JN248 with high phenylpyruvate reductive activity (2017), J. Food Sci., 82, 2269-2275 .
    View publication on PubMed
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Limosilactobacillus fermentum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 NADH pH 8, 50°C Limosilactobacillus fermentum
1.68
-
 phenylpyruvate pH 8, 50°C Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum
-
-
-
Limosilactobacillus fermentum JN248
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NADH + H+ 12.6% of the activity with pyruvate Limosilactobacillus fermentum (R)-2-hydroxybutanoate + NAD+
-
 ?
2-oxobutanoate + NADH + H+ 12.6% of the activity with pyruvate Limosilactobacillus fermentum JN248 (R)-2-hydroxybutanoate + NAD+
-
 ?
3,4-dihydroxyphenylpyruvate + NADH + H+ 43.1% of the activity with pyruvate Limosilactobacillus fermentum (R)-3,4-dihydroxyphenyllactate + NAD+
-
 ?
3,4-dihydroxyphenylpyruvate + NADH + H+ 43.1% of the activity with pyruvate Limosilactobacillus fermentum JN248 (R)-3,4-dihydroxyphenyllactate + NAD+
-
 ?
3-methyl-2-oxobutanoate + NADH + H+ 19.4% of the activity with pyruvate Limosilactobacillus fermentum (R)-2-hydroxy-3-methylbutanoate + NAD+
-
 ?
additional information enzyme displays more than 99% enantioselectivity. No substrates: 3-methyl-2-oxopentanoate, 4-methyl-2-oxopentanoate, indole-3-pyruvate Limosilactobacillus fermentum ?
-
-
additional information enzyme displays more than 99% enantioselectivity. No substrates: 3-methyl-2-oxopentanoate, 4-methyl-2-oxopentanoate, indole-3-pyruvate Limosilactobacillus fermentum JN248 ?
-
-
phenylpyruvate + NADH + H+ 70.4% of the activity with pyruvate Limosilactobacillus fermentum (R)-3-phenyllactate + NAD+
-
 ?
phenylpyruvate + NADH + H+ 70.4% of the activity with pyruvate Limosilactobacillus fermentum JN248 (R)-3-phenyllactate + NAD+
-
 ?
pyruvate + NADH + H+
-
 Limosilactobacillus fermentum (R)-lactate + NAD+
-
 ?
pyruvate + NADH + H+
-
 Limosilactobacillus fermentum JN248 (R)-lactate + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
D-LDH0653
-
 Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Limosilactobacillus fermentum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
67.4
-
 NADH pH 8, 50°C Limosilactobacillus fermentum
122.7
-
 phenylpyruvate pH 8, 50°C Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Limosilactobacillus fermentum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 90% of maximum activity Limosilactobacillus fermentum
10
-
 70% residual activity Limosilactobacillus fermentum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
56.16
-
 NADH pH 8, 50°C Limosilactobacillus fermentum
73.01
-
 phenylpyruvate pH 8, 50°C Limosilactobacillus fermentum