Information on EC 2.6.1.7 - kynurenine-oxoglutarate transaminase

Word Map on EC 2.6.1.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.6.1.7
-
RECOMMENDED NAME
GeneOntology No.
kynurenine-oxoglutarate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-tryptophan degradation XI (mammalian, via kynurenine)
-
-
Metabolic pathways
-
-
Tryptophan metabolism
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-kynurenine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on 3-hydroxykynurenine. The product 4-(2-aminophenyl)-2,4-dioxobutanoate is converted into kynurenate by a spontaneous reaction.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-38-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
yeast
-
-
Manually annotated by BRENDA team
Hansenula schneggii
yeast
-
-
Manually annotated by BRENDA team
isoforms KAT I and KAT II, no change of enzyme activity 24 and 72 h after transient global ischemia episode
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
black tiger prawn
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
low activity
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobutyrate + glyoxylate
?
show the reaction diagram
-
-
-
-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
slightly better than L-kynurenine
-
?
3-hydroxykynurenine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
3-hydroxykynurenine + pyruvate
?
show the reaction diagram
-
-
-
?
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate
2-oxoheptane-1,7-dioate + L-glutamate
show the reaction diagram
-
at 39.7% of the activity with 2-aminohexane-1,6-dioate
-
?
L-2-aminohexane-1,6-dioate + 2-oxoglutarate
2-oxohexane-1,6-dioate + L-glutamate
show the reaction diagram
Hansenula schneggii
-
at about 60% the rate of L-kynurenine
-
?
L-3-hydroxykynurenine + 2-oxoglutarate
xanthurenic acid + L-glutamate
show the reaction diagram
L-4-chloro-kynurenine + 2-oxoglutarate
L-7-chloro-kynurenic acid + L-glutamate
show the reaction diagram
-
-
-
?
L-5-chloro-kynurenine + 2-oxoglutarate
L-6-chloro-kynurenic acid + L-glutamate
show the reaction diagram
-
-
-
?
L-alanine + 2-oxobutanoate
pyruvate + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-alanine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxobutanoate
2-oxosuccinamic acid + 2-aminobutanoate
show the reaction diagram
L-asparagine + glyoxylate
?
show the reaction diagram
-
KAT II has high transamination activity toward L-asparagine
-
-
?
L-cysteine + 2-oxobutanoate
2-aminobutanoate + 3-mercapto-2-oxopropanoate
show the reaction diagram
L-cysteine + 2-oxobutanoate
? + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-cysteine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-glutamine + 2-oxobutanoate
2-oxoglutarate + 2-aminobutanoate
show the reaction diagram
L-glutamine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-histidine + 2-oxobutanoate
3-(1H-imidazol-4-yl)-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-histidine + 2-oxoglutarate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-histidine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxo-4-methylthiobutyrate
?
show the reaction diagram
L-kynurenine + 2-oxo-4-methylthiobutyrate
kynurenate + L-methionine
show the reaction diagram
L-kynurenine + 2-oxo-4-methylthiobutyrate
kynurenic acid + L-methionine
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxoadipate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxoadipate
kynurenic acid + 2-aminoadipate + H2O
show the reaction diagram
L-kynurenine + 2-oxobutanoate
4-(2-aminophenyl)-2,4-dioxobutanoate + 2-aminobutanoate
show the reaction diagram
L-kynurenine + 2-oxobutyrate
?
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxobutyrate
kynurenate + ?
show the reaction diagram
L-kynurenine + 2-oxobutyrate
kynurenic acid + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxobutyrate
kynurenic acid + 2-aminobutanoate + H2O
show the reaction diagram
L-kynurenine + 2-oxocaproate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxocaproic acid
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxocaproic acid
kynurenic acid + 2-aminohexanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 2-oxoglutarate
4-(2-aminophenyl)2,4-dioxobutanoate + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
?
show the reaction diagram
-
poor cosubstrate for KAT III
-
-
?
L-kynurenine + 2-oxoglutarate
kynurenate + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate
show the reaction diagram
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate + H2O
show the reaction diagram
L-kynurenine + 2-oxohexanedioate
kynurenic acid + 2-aminohexanedioate + H2O
show the reaction diagram
L-kynurenine + 2-oxohexanoate
kynurenic acid + L-norleucine + H2O
show the reaction diagram
Hansenula schneggii
-
-
-
?
L-kynurenine + 2-oxoisocaproate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-leucine
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxoisohexanoate
kynurenic acid + 2-aminoisohexanoate + H2O
show the reaction diagram
L-kynurenine + 2-oxoisopentanoate
kynurenic acid + 2-aminoisopentanoate + H2O
show the reaction diagram
-
poor substrate
-
?
L-kynurenine + 2-oxomethylthiobutyric acid
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 2-oxopentanoate
kynurenic acid + L-norvaline + H2O
show the reaction diagram
L-kynurenine + 2-oxovalerate
?
show the reaction diagram
L-kynurenine + 2-oxovalerate
kynurenic acid + 2-aminopentanoate
show the reaction diagram
-
-
-
?
L-kynurenine + 3-indole-2-oxopropanoate
kynurenic acid + L-tryptophan + H2O
show the reaction diagram
L-kynurenine + 3-methyl-2-oxobutanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 3-methyl-2-oxopentanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 3-methyl-2-oxopentanoate
kynurenic acid + L-isoleucine + H2O
show the reaction diagram
-
poor substrate
-
?
L-kynurenine + 3-phenyl-2-oxopropanoate
kynurenic acid + L-phenylalanine + H2O
show the reaction diagram
L-kynurenine + 4-methyl-2-oxopentanoate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + 4-methyl-2-oxovalerate
kynurenic acid + 2,4-dimethylvalerate + H2O
show the reaction diagram
5.9% of activity with 2-oxoglutarate
-
?
L-kynurenine + 4-methylsulfanyl-2-oxobutyrate
kynurenic acid + L-methionine + H2O
show the reaction diagram
L-kynurenine + glyoxylate
4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
show the reaction diagram
L-kynurenine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + glyoxylate
kynurenic acid + glycine
show the reaction diagram
L-kynurenine + glyoxylate
kynurenic acid + glycine + H2O
show the reaction diagram
L-kynurenine + hydroxyphenylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + hydroxyphenylpyruvate
kynurenic acid + L-tyrosine
show the reaction diagram
-
-
-
?
L-kynurenine + indo-3-pyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + indol-3-ylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + indole-3-pyruvate
kynurenic acid + L-tryptophan
show the reaction diagram
-
-
-
?
L-kynurenine + mercaptopyruvate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-cysteine
show the reaction diagram
-
-
-
-
?
L-kynurenine + mercaptopyruvate
?
show the reaction diagram
L-kynurenine + mercaptopyruvate
kynurenic acid + ?
show the reaction diagram
-
-
-
?
L-kynurenine + oxaloacetate
4-(2-aminophenyl)-2,4-dioxobutanoate + 2-aminosuccinate
show the reaction diagram
-
-
-
-
?
L-kynurenine + oxaloacetate
?
show the reaction diagram
L-kynurenine + oxaloacetate
kynurenate + L-aspartate
show the reaction diagram
L-kynurenine + oxaloacetate
kynurenic acid + L-aspartate
show the reaction diagram
-
-
-
?
L-kynurenine + oxaloacetate
kynurenic acid + L-aspartate + H2O
show the reaction diagram
L-kynurenine + p-hydroxyphenylpyruvate
?
show the reaction diagram
-
-
-
-
?
L-kynurenine + phenylpyruvate
4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + phenylpyruvate
?
show the reaction diagram
L-kynurenine + phenylpyruvate
kynurenic acid + L-phenylalanine
show the reaction diagram
-
-
-
?
L-kynurenine + phenylpyruvate
phenylalanine + L-glutamate + H2O
show the reaction diagram
43% of activity with 2-oxoglutarate
-
?
L-kynurenine + pyruvate
4-(2-aminophenyl)-2,4-dioxobutanoic acid + L-alanine
show the reaction diagram
-
-
-
-
ir
L-kynurenine + pyruvate
4-(2-aminophenyl)2,4-dioxobutanoate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + pyruvate
?
show the reaction diagram
L-kynurenine + pyruvate
kynurenic acid + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + pyruvate
kynurenic acid + L-alanine + H2O
show the reaction diagram
L-leucine + 2-oxobutanoate
2-oxoisocaproate + 2-aminobutanoate
show the reaction diagram
-
-
-
-
?
L-leucine + 2-oxobutanoate
alpha-ketoleucine + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
L-lysine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-methionine + 2-oxobutanoate
4-methylsulfanyl-2-oxobutanoate + 2-aminobutanoate
show the reaction diagram
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + glutamate
show the reaction diagram
L-methionine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-norleucine + 2-oxoglutarate
2-oxohexanoate + L-glutamate
show the reaction diagram
-
at about 30% the rate of L-kynurenine
-
?
L-phenylalanine + 2-oxobutanoate
phenylpyruvate + 2-aminobutanoate
show the reaction diagram
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
L-phenylalanine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-serine + 2-oxobutanoate
3-hydroxy-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
-
-
-
?
L-serine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-tryptophan + 2-oxobutanoate
3-indole-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
L-tryptophan + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-tyrosine + 2-oxobutanoate
3-(4-hydroxyphenyl)-2-oxopropanoate + 2-aminobutanoate
show the reaction diagram
L-tyrosine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
L-tyrosine + glyoxylate
?
show the reaction diagram
-
-
-
-
?
L-vinylglycine
2-oxobutyrate + NH3
show the reaction diagram
Hansenula schneggii
-
deamination at 1% the rate of kynurenine, suicide substrate
-
?
L-vinylglycine + 1-propanethiol
S-n-propylhomocysteine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
-
?
L-vinylglycine + 2-oxobutyrate
2-aminobutyrate + 2-oxo-3-butenoate
show the reaction diagram
Hansenula schneggii
-
-
-
?
L-vinylglycine + ethanethiol
ethionine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
i.e. S-ethylhomocysteine
?
L-vinylglycine + methanethiol
methionine
show the reaction diagram
Hansenula schneggii
-
gamma-addition reaction in the presence of alkanethiols
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-kynurenine + 2-oxoglutarate
kynurenic acid + L-glutamate + H2O
show the reaction diagram
additional information
?
-
B1B6U5
the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
additional information
-
pyridoxamine 5'-phosphate is not a cofactor
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
approx. 4fold increase in activity at pH 6.4
additional information
-
not activated by chloride salts of Mg2+, Mn2+, Na+, K+ or NH4+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3S)-3-amino-1-hydroxy-3,4-dihydroquinolin-2(1H)-one
(3S)-3-amino-6-benzyl-1-hydroxy-7-methoxy-3,4-dihydroquinolin-2(1H)-one
-
-
(R)-2-amino-4-(4-(ethylsulfonyl)phenyl)-4-oxobutanoic acid
-
71% inhibition at 1 mM, 63% inhibition at 0.1 mM, poor substrate that undergo slow transamination, KM-value 4.5 mM, Kcat-value 0.15 1/sec, pH 7.5, 25C
(S)-(-)-9-(4-aminopiperazin-1-yl)-8-fluoro-3-methyl-6-oxo-2,3-dihydro-6H-1-oxa-3a-azaphenalene-5-carboxylic acid
-
BFF-122
(S)-(4)-(ethylsulfonyl)benzoylalanine
-
no inhibition of the human enzyme
(S)-4-ethylsulfonylbenzoylalanine
-
specific inhibitor of KAT II
(S)-9-(4-aminopiperazine-1-yl)-8-fluoro-3-methyl-6-oxo-2,3,5,6-tetrahydro-4H-1-oxa-3a-azaphenalene-5-carboxylic acid
-
BFF 122, KAT II activity is totally blocked at 0.01 mM, 43% inhibition of KAT II at 0.001 mM, 26% inhibition of KAT I at 1 mM
2-Aminoadipate
-
-
2-oxoadipate
-
-
2-oxoglutarate
3-(2-carboxyethyl)-5-chloro-1H-indole-2-carboxylic acid
-
-
3-(2-carboxyethyl)-5-nitro-1H-indole-2-carboxylic acid
-
-
3-hydroxykynurenine
3-indolepropionic acid
3-Methyl-2-benzothiazolone hydrazone hydrochloride
Hansenula schneggii
-
0.1 mM, complete inhibition, reversible by pyridoxal phosphate
4-carboxy-3-hydroxyphenylglycine
-
inhibition of brain KAT II
4-chloromercuribenzoate
5-(2-(3-chlorophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-(2-(4-bromophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-(2-(4-chlorophenyl) hydrazono)-6-ethoxy-6-oxohexanoic acid
-
-
5-bromo-3-(2-carboxyethyl)-1H-indole-2-carboxylic acid
-
-
6-ethoxy-5-(2-(4-iodophenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-5-(2-(4-methoxyphenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-5-(2-(4-nitrophenyl)hydrazono)-6-oxohexanoic acid
-
-
6-ethoxy-6-oxo-5-(2-p-tolylhydrazono)hexanoic acid
-
-
adipate
alpha-aminoadipate
-
strong inhibition of KAT II
amino-2,3-dihydro-5-methyl-3-oxo-4-isoxazolepropionic acid
-
inhibitory in whole cells
aminoadipate
aminooxyacetic acid
-
inhibitory in whole cells
anthranilate
-
weak inhibition
azelaic acid
-
-
cysteine
cysteine sulfinate
-
poor substrate that undergoes slow transamination, KM-value 12.3 mM, pH 7.5, 25C
Decanoic acid
-
-
Dicarboxylic acids
Diethylglutaric acid
-
-
Dimethylglutaric acid
-
-
-
DL-indole-3-lactic acid
EGTA
-
strong inhibition at pH 6.8 and Ca2+ concentrations below 0.5 mM
glutamate
glutamine
hydroxylamine
indole-3-acetic acid
-
-
Indole-3-propionic acid
Indole-3-pyruvate
-
5 mM, complete inhibition
indole-3-pyruvic acid
-
-
iodoacetate
Isonicotinic acid hydrazide
-
anti-tuberculosis drug, enzyme is inhibited in animals fed with a diet containing isonicotinic acid hydrazide and by injection of the drug
KCN
-
complete inhibition
Kynurenate
-
weak inhibition
L- glutamate
-
5 mM decreases KAT II activity
L-2-amino-4-phosphonobutyric acid
-
inhibition of brain KAT II
L-2-aminoadipate
-
-
L-4-chlorokynurenine
-
-
L-5-chlorokynurenine
-
-
L-asparagine
L-aspartate
L-Cycloserine
Hansenula schneggii
-
0.1 mM, 88% inhibition, reversible by pyridoxal phosphate
L-cysteate
-
5.38 mM, 50% inhibition of brain KAT I, 1.55 mM, 50% inhibition of brain KAT II
L-cysteine
L-cysteine sulfinate
-
0.002 mM, 50% inhibition of brain KAT II
L-glutamate
L-glutamine
L-histidine
L-Homocysteate
-
5.0 mM, 50% inhibition of brain KAT I, 2.1 mM, 50% inhibition of brain KAT II
L-Homocysteine sulfinate
-
1.59 mM, 50% inhibition of brain KAT II
L-kynurenine
L-leucine
L-lysine
L-methionine
L-Penicillamine
Hansenula schneggii
-
0.1 mM, 62% inhibition, reversible by pyridoxal phosphate
L-phenylalanine
L-serine-O-phosphate
-
inhibition of brain KAT II
L-tryptophan
methionine
N-ethylmaleimide
Hansenula schneggii
-
-
N-omega-nitro-L-arginine
-
-
nicotinate
-
weak inhibition
phenylalanine
;
phenylhydrazine
Hansenula schneggii
-
0.1 mM, 81% inhibition, reversible by pyridoxal phosphate
phosphate
-
-
pimelate
pyridoxal 5'-phosphate
-
at high concentrations
quinolinic acid
-
strong inhibition at pH 6.8 and Ca2+ concentrations below 0.5 mM
quisqualate
Quisqualic acid
-
1 mM, isoform KAT I, 81% residual activity, isoform KAT II, 1% residual activity
S-dichlorovinyl-L-cysteine
Sulfate or phosphate esters of steroids
-
-
-
trans-3-indolacrylic acid
-
-
trans-pyrrolidine-2,4-dicarboxylate
-
inhibition of brain KAT II
-
Tris amine
-
the commonly used buffer for KAT I assays greatly inhibits KAT I around neutral pH conditions, but shows no inhibition at basic pH condition
tryptophan
xanthurenate
-
weak inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
L-cysteine
at 1.25 mM, 3fold stimulation, at 10 mM, 70% inhibition
L-kynurenine
-
allosterically regulated
L-tryptophan
-
2 mM, 84% increase in activity of heart KAT at pH 8.0
pyridoxamine 5'-phosphate
Hansenula schneggii
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21.3 - 92.7
2-aminobutanoate
0.00241 - 5.7
2-oxo-4-methylthiobutyrate
0.01 - 20.9
2-oxoadipate
3
2-oxobutanoate
-
pH 7.5, 45C
0.000217 - 42.2
2-oxobutyrate
1.5
2-oxocaproate
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.5 - 10.4
2-oxocaproic acid
0.013 - 8.1
2-oxoglutarate
1
2-oxoisocaproate
5.3
2-oxoisocaproic acid
-
in 100 mM boric acid buffer, pH 9.0, at 45C
1.2 - 10.9
2-oxovalerate
1.9
3-(2-aminobenzoyl)-L-alanine
Hansenula schneggii
-
pH 8.0, 37C
0.2062
3-hydroxykynurenine
in 200 mM phosphate buffer, pH 7.5, at 37C
12.9 - 14.2
3-methyl-2-oxobutanoate
3.3
4-methyl-2-oxopentanoate
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.4
alpha-oxo-gamma-methiol-butyric acid
-
in 100 mM boric acid buffer, pH 9.0, at 45C
1.2
beta-phenylpyruvate
0.4 - 18
glyoxylate
1 - 1.6
hydroxyphenylpyruvate
0.5 - 1.4
indo-3-pyruvate
3.6
Indole-3-pyruvate
-
pH 7.5, 38C
1.36 - 6.3
L-3-hydroxykynurenine
6.2 - 246
L-alanine
1.4 - 23.1
L-asparagine
0.7 - 1.3
L-cysteine
5.6
L-glutamate
-
pH 8.0, 37C, alpha-aminoadipate aminotransferase activity
0.7 - 3.8
L-glutamine
0.7 - 5.4
L-histidine
0.083 - 27
L-kynurenine
5 - 34.5
L-leucine
0.9 - 6.4
L-methionine
1.1 - 3.5
L-phenylalanine
3 - 32.7
L-serine
1.2 - 12.9
L-tryptophan
0.9 - 3.2
L-tyrosine
2.7
L-Vinylglycine
Hansenula schneggii
-
pH 8.0. 25C
2.4 - 3.2
Mercaptopyruvate
0.9 - 16.8
oxaloacetate
0.6 - 1.8
phenylpyruvate
0.00085 - 0.0013
pyridoxal 5'-phosphate
0.0032
pyridoxamine 5'-phosphate
Hansenula schneggii
-
pH 8.0, 37C
0.0175 - 12.1
pyruvate
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
38.3 - 317
2-aminobutanoate
0.16 - 4.27
2-oxo-4-methylthiobutyrate
4.84
2-oxoadipate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
234
2-oxobutanoate
Homo sapiens
-
pH 7.5, 45C
0.0018 - 3.49
2-oxobutyrate
4.83
2-oxocaproate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.41 - 2.59
2-oxocaproic acid
0.37 - 7.67
2-oxoglutarate
296
2-oxoisocaproate
Homo sapiens
-
pH 7.5, 45C
0.74
2-oxoisocaproic acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
0.13 - 2.7
2-oxovalerate
65.12
3-hydroxykynurenine
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH 7.5, at 37C
0.92
3-methyl-2-oxobutanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
0.92
3-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
2.51
4-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1.02
alpha-oxo-gamma-methiol-butyric acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
0.19 - 263
glyoxylate
0.39 - 1.48
hydroxyphenylpyruvate
1.03 - 1.49
indo-3-pyruvate
0.49
Indole-3-pyruvate
Mus musculus
-
pH 7.5, 38C
1.53 - 1540
L-alanine
2.93 - 230
L-asparagine
1.3 - 206
L-cysteine
2.27 - 561
L-glutamine
2 - 168
L-histidine
1.8 - 201
L-kynurenine
339 - 758
L-leucine
2.43 - 215
L-methionine
2.7 - 283
L-phenylalanine
2.17 - 345
L-serine
3.57 - 283
L-tryptophan
1.03 - 139
L-tyrosine
0.44 - 234
Mercaptopyruvate
0.065 - 143
oxaloacetate
0.63 - 110
phenylpyruvate
0.36 - 29
pyruvate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07 - 1.75
2-oxo-4-methylthiobutyrate
5100
0.23
2-oxoadipate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1184
0.02 - 8.2
2-oxobutyrate
371
3.13
2-oxocaproate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
3676
0.04 - 5.18
2-oxocaproic acid
14934
0.045 - 6.24
2-oxoglutarate
34
0.14
2-oxoisocaproic acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
27233
0.01 - 2.25
2-oxovalerate
1316
315
3-hydroxykynurenine
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH7.5, at 37C
2461
0.07
3-methyl-2-oxobutanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
829
0.065
3-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
1133
0.76
4-methyl-2-oxopentanoate
Homo sapiens
-
in 100 mM potassium phosphate buffer (pH 7.4), at 37C
722
36.8
alpha-keto-gamma-methylthiobutyrate
Haemaphysalis longicornis
B1B6U5
in 200 mM phosphate buffer, pH7.5, at 37C
177096
2.54
alpha-oxo-gamma-methiol-butyric acid
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
27232
0.05 - 6.78
glyoxylate
101
0.26 - 1.48
hydroxyphenylpyruvate
3899
1.08 - 2.07
indo-3-pyruvate
12081
0.13
Indole-3-pyruvate
Mus musculus
-
pH 7.5, 38C
2408
0.25
L-alanine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
103
2.095
L-asparagine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
266
1.86
L-cysteine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
74
3.24
L-glutamine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
102
2.86
L-histidine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
349
1.53 - 500
L-kynurenine
409
2.7
L-methionine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
88
2.45
L-phenylalanine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
104
0.72
L-serine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
95
0.5
L-tryptophan
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
119
0.38
L-tyrosine
Mus musculus
-
in 100 mM boric acid buffer, pH 9.0, at 45C
109
0.14 - 1.37
Mercaptopyruvate
5576
0.09 - 0.75
oxaloacetate
57
0.96 - 4.22
phenylpyruvate
198
0.04 - 116
pyruvate
31
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02011
2-oxoglutarate
-
pH 7.5, 25C
13
adipate
Hansenula schneggii
-
-
0.013
cysteine sulfinate
-
17.2 mM dissociation constant for dissociation of the inhibitor from the enzyme substrate complex, pH 7.5, 25C
9.5
pimelate
Hansenula schneggii
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000015 - 0.00003
(3S)-3-amino-1-hydroxy-3,4-dihydroquinolin-2(1H)-one
0.000004 - 0.000005
(3S)-3-amino-6-benzyl-1-hydroxy-7-methoxy-3,4-dihydroquinolin-2(1H)-one
0.00091
(S)-(-)-9-(4-aminopiperazin-1-yl)-8-fluoro-3-methyl-6-oxo-2,3-dihydro-6H-1-oxa-3a-azaphenalene-5-carboxylic acid
Homo sapiens
-
0.0015 mM or the two enantiomers, pH 8.0, 37C
0.293
3-(2-carboxyethyl)-5-chloro-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.666
3-(2-carboxyethyl)-5-nitro-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.02
5-(2-(3-chlorophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.021
5-(2-(4-bromophenyl)hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.02
5-(2-(4-chlorophenyl) hydrazono)-6-ethoxy-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.271
5-bromo-3-(2-carboxyethyl)-1H-indole-2-carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.034
6-ethoxy-5-(2-(4-iodophenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.043
6-ethoxy-5-(2-(4-methoxyphenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.179
6-ethoxy-5-(2-(4-nitrophenyl)hydrazono)-6-oxohexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.033
6-ethoxy-6-oxo-5-(2-p-tolylhydrazono)hexanoic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.002
cysteine sulfinate
Homo sapiens
-
pH 7.5, 25C
0.14
Indole-3-propionic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication