Application | Comment | Organism |
---|---|---|
analysis | development of a coupled fluorescence assay for human KATII with short assay time | Homo sapiens |
analysis | development of a sensitive, high-throughput, label-free RapidFire mass spectrometry assay for kynurenine aminotransferase. The method is directly applicable to KATII enzymes from different animal species, allowing to select proper animal model(s) to evaluate human KATII inhibitors | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Sf9 cell | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3S)-3-amino-1-hydroxy-3,4-dihydroquinolin-2(1H)-one | - |
Homo sapiens | |
(3S)-3-amino-6-benzyl-1-hydroxy-7-methoxy-3,4-dihydroquinolin-2(1H)-one | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
L-kynurenine | mass spectrometry assay, pH 7.5, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N5Z0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + 2-oxoglutarate | - |
Homo sapiens | 4-(2-aminophenyl)2,4-dioxobutanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KAT2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
L-kynurenine | mass spectrometry assay, pH 7.5, temperature not specified in the publication | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000004 | - |
mass spectrometry assay, pH 7.5, temperature not specified in the publication | Homo sapiens | (3S)-3-amino-6-benzyl-1-hydroxy-7-methoxy-3,4-dihydroquinolin-2(1H)-one | |
0.000005 | - |
fluorescence assay, pH 7.5, temperature not specified in the publication | Homo sapiens | (3S)-3-amino-6-benzyl-1-hydroxy-7-methoxy-3,4-dihydroquinolin-2(1H)-one | |
0.000015 | - |
flourescence assay, pH 7.5, temperature not specified in the publication | Homo sapiens | (3S)-3-amino-1-hydroxy-3,4-dihydroquinolin-2(1H)-one | |
0.00003 | - |
mass spectrometry assay, pH 7.5, temperature not specified in the publication | Homo sapiens | (3S)-3-amino-1-hydroxy-3,4-dihydroquinolin-2(1H)-one |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
L-kynurenine | mass spectrometry assay, pH 7.5, temperature not specified in the publication | Homo sapiens |