Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.7 extracted from

  • Han, Q.; Robinson, H.; Cai, T.; Tagle, D.A.; Li, J.
    Biochemical and structural characterization of mouse mitochondrial aspartate aminotransferase, a newly identified kynurenine aminotransferase-IV (2011), Biosci. Rep., 31, 323-332.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
chitin-binding domain fusion proteinfusionexpressed in Escherichia coli Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method, PEG 4000, Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
phenylpyruvate pH 7.5, 38°C Mus musculus
0.9
-
oxaloacetate pH 7.5, 38°C Mus musculus
1.6
-
hydroxyphenylpyruvate pH 7.5, 38°C Mus musculus
2.4
-
2-oxoglutarate pH 7.5, 38°C Mus musculus
3.2
-
Mercaptopyruvate pH 7.5, 38°C Mus musculus
3.6
-
Indole-3-pyruvate pH 7.5, 38°C Mus musculus
4.2
-
glyoxylate pH 7.5, 38°C Mus musculus
5.7
-
2-oxo-4-methylthiobutyrate pH 7.5, 38°C Mus musculus
8.3
-
pyruvate pH 7.5, 38°C Mus musculus
10.4
-
2-oxocaproic acid pH 7.5, 38°C Mus musculus
10.9
-
2-oxovalerate pH 7.5, 38°C Mus musculus
42.2
-
2-oxobutyrate pH 7.5, 38°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Mus musculus 5739
-

Organism

Organism UniProt Comment Textmining
Mus musculus P05202
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, ion exchange chromatography (Q-Sepharose), gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + 2-oxo-4-methylthiobutyrate
-
Mus musculus kynurenic acid + L-methionine
-
?
L-kynurenine + 2-oxobutyrate
-
Mus musculus kynurenic acid + 2-aminobutanoate
-
?
L-kynurenine + 2-oxocaproic acid
-
Mus musculus kynurenic acid + 2-aminohexanoate
-
?
L-kynurenine + 2-oxoglutarate
-
Mus musculus kynurenic acid + L-glutamate + H2O
-
?
L-kynurenine + 2-oxovalerate
-
Mus musculus kynurenic acid + 2-aminopentanoate
-
?
L-kynurenine + glyoxylate
-
Mus musculus kynurenic acid + glycine + H2O
-
?
L-kynurenine + hydroxyphenylpyruvate
-
Mus musculus kynurenic acid + L-tyrosine
-
?
L-kynurenine + indole-3-pyruvate
-
Mus musculus kynurenic acid + L-tryptophan
-
?
L-kynurenine + mercaptopyruvate
-
Mus musculus kynurenic acid + ?
-
?
L-kynurenine + oxaloacetate
-
Mus musculus kynurenic acid + L-aspartate + H2O
-
?
L-kynurenine + phenylpyruvate
-
Mus musculus kynurenic acid + L-phenylalanine
-
?
L-kynurenine + pyruvate
-
Mus musculus kynurenic acid + L-alanine + H2O
-
?

Subunits

Subunits Comment Organism
homodimer crystal structure analysis Mus musculus

Synonyms

Synonyms Comment Organism
aspartate aminotransferase
-
Mus musculus
kynurenine aminotransferase-IV
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
2-oxovalerate pH 7.5, 38°C Mus musculus
0.19
-
glyoxylate pH 7.5, 38°C Mus musculus
0.32
-
oxaloacetate pH 7.5, 38°C Mus musculus
0.37
-
pyruvate pH 7.5, 38°C Mus musculus
0.41
-
hydroxyphenylpyruvate pH 7.5, 38°C Mus musculus
0.41
-
2-oxo-4-methylthiobutyrate pH 7.5, 38°C Mus musculus
0.41
-
2-oxocaproic acid pH 7.5, 38°C Mus musculus
0.44
-
Mercaptopyruvate pH 7.5, 38°C Mus musculus
0.49
-
Indole-3-pyruvate pH 7.5, 38°C Mus musculus
0.53
-
2-oxoglutarate pH 7.5, 38°C Mus musculus
0.63
-
phenylpyruvate pH 7.5, 38°C Mus musculus
0.69
-
2-oxobutyrate pH 7.5, 38°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
2-oxovalerate pH 7.5, 38°C Mus musculus
0.02
-
2-oxobutyrate pH 7.5, 38°C Mus musculus
0.04
-
2-oxocaproic acid pH 7.5, 38°C Mus musculus
0.05
-
pyruvate pH 7.5, 38°C Mus musculus
0.05
-
glyoxylate pH 7.5, 38°C Mus musculus
0.07
-
2-oxo-4-methylthiobutyrate pH 7.5, 38°C Mus musculus
0.13
-
Indole-3-pyruvate pH 7.5, 38°C Mus musculus
0.14
-
Mercaptopyruvate pH 7.5, 38°C Mus musculus
0.22
-
2-oxoglutarate pH 7.5, 38°C Mus musculus
0.26
-
hydroxyphenylpyruvate pH 7.5, 38°C Mus musculus
0.35
-
oxaloacetate pH 7.5, 38°C Mus musculus
0.96
-
phenylpyruvate pH 7.5, 38°C Mus musculus