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0.00012
2'-deoxyguanosine triphosphate
-
in 50 mM Tris-HCl (pH 7.6), 100 mM KCl, 5 mM MgCl2, 10% (v/v) glycerol, 1 mM dithiothreitol, at 30°C
0.006 - 0.0144
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
0.0151
5-ethynyl-dCTP
-
pH and temperature not specified in the publication
-
0.0063
5-ethynyl-dUTP
-
pH and temperature not specified in the publication
-
0.013 - 1.22
5-Methyl-dCTP
0.006
5-phenyl-dCTP
-
pH and temperature not specified in the publication
-
0.0079
5-phenyl-dUTP
-
pH and temperature not specified in the publication
-
0.0141
5-vinyl-dCTP
-
pH and temperature not specified in the publication
-
0.0131
5-vinyl-dUTP
-
pH and temperature not specified in the publication
-
0.0003
5-[(1E)-3-[(4-[[(5-azido-2-nitrophenyl)carbonyl]amino]butanoyl)amino]prop-1-en-1-yl]uridine 5'-triphosphate
-
pH 9.0, 25°C
0.0055
5-[(1E)-3-{[(5-azido-2-nitrophenyl)carbonyl]amino}prop-1-en-1-yl]-2'-deoxyuridine 5'-triphosphate
-
pH 9.0, 25°C
0.04
5-[N-(2-nitro-5-azidobenzoyl)ami-nomethyl]-2'-deoxyuridine 5'-triphosphate
-
pH 9.0, 25°C
0.0011 - 0.344
7-deaza-2'-deoxyadenosine 5'-triphosphate
0.0125
7-deaza-dGTP
-
pH and temperature not specified in the publication
0.0073
7-ethynyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
0.0075
7-ethynyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
0.0061
7-methyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
0.0111
7-methyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
0.0056
7-phenyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
0.0041
7-phenyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
0.0109
7-vinyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
0.0095
7-vinyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
0.00623
Cy3-dATP
-
pH 8.0, 25°C
-
0.003
Cy3-dCTP
-
pH 8.0, 25°C
-
0.0112
Cy3-dGTP
-
pH 8.0, 25°C
-
0.00893
Cy3-dUTP
-
pH 8.0, 25°C
-
0.1056
dAMP:dA
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template A
-
0.551
dCMP:dA
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template A
-
0.00002772 - 0.00002847
dCMP:dG
-
0.00118 - 0.64
deoxynucleoside triphosphate
2.091
dGMP:dA
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template A
-
0.16
dPTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.00003176
dTMP:dA
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template A
-
0.223 - 0.403
N1-methyl-2'-deoxyadenosine 5'-triphosphate
0.0012 - 0.0087
North-methanocarba-dATP
0.0013
South-methanocarba-dATP
pH 7.5, 37°C
0.0032
TTP
-
pH and temperature not specified in the publication
additional information
additional information
-
0.006
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.0144
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.067
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.98
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.013
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
1.22
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.0011
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.0024
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.0028
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.297
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
0.344
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
1.153
dAMP:dG
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template G
-
1.42
dAMP:dG
-
37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G
-
0.00028
dATP
-
pH 8.0, 25°C
0.0008
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.0014
dATP
pH 7.5, 37°C
0.002
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409M
0.0025
dATP
-
55°C, pH not specified in the publication, wild-type enzyme
0.0028
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.0032
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.007
dATP
pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.0087
dATP
-
pH and temperature not specified in the publication
0.0088
dATP
pH 7.8, 37°C, dATP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
0.01
dATP
-
55°C, pH not specified in the publication, mutant enzyme A408S
0.011
dATP
-
template base: dT, pH 7.5, 37°C
0.012
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409V
0.014
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409I
0.02
dATP
pH 7.8, 37°C, incorporation of dATP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
0.022
dATP
-
pH 9.0, 37°C, recombinant mutant enzyme
0.022
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.03
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
0.046
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410V
0.069
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410L
0.13
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410I
0.18
dATP
pH 7.8, 37°C, incorporation of dATP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.2
dATP
-
template base: dI, pH 7.5, 37°C
0.205
dATP
pH 7.5, 60°C, incorporation opposite an abasic site
0.253
dATP
-
pH 8.0, 37°C, recombinant enzyme
0.274
dATP
-
pH 8.0, 50°C, recombinant enzyme
0.325
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.39
dATP
pH 7.5, 37°C, DNA polymerase Dpo3
0.43
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.49
dATP
-
template base: dG, pH 7.5, 37°C
0.59
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.69
dATP
pH 7.5, 37°C, DNA polymerase Dpo4
0.72
dATP
-
template base: dX, pH 7.5, 37°C
0.74
dATP
-
pH and temperature not specified in the publication
0.77
dATP
-
pH and temperature not specified in the publication
0.79
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.84
dATP
-
template base: dA, pH 7.5, 37°C
0.88
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
1.1
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
1.2
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
1.5
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
1.9
dATP
-
template base: dC, pH 7.5, 37°C
2.1
dATP
pH 7.5, 37°C, DNA polymerase Dpo1
2.2
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
2.3
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
2.5
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
2.8
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
3.2
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.00002772
dCMP:dG
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template G
-
0.00002847
dCMP:dG
-
37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G
-
0.000077
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.000098
dCTP
pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.0002
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
0.00027
dCTP
-
pH 8.0, 25°C
0.00044
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
0.00071
dCTP
pH 7.8, 37°C, dCTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
0.00249
dCTP
-
mutant L606K, pH 7.5, 37°C, misincorporation of dTTP with G at first position
0.0025
dCTP
-
template base: dG, pH 7.5, 37°C
0.0041
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.00464
dCTP
-
wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position
0.0062
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0067
dCTP
-
pH and temperature not specified in the publication
0.0068
dCTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
0.0077
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.00792
dCTP
-
mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position
0.0084
dCTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.0098
dCTP
pH 7.5, 37°C, DNA polymerase Dpo2
0.01
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.012
dCTP
37°C, pH 7.4, mutant enzyme T239W
0.012
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.014
dCTP
37°C, pH 7.4, mutant enzyme N188W
0.017
dCTP
-
template base: dI, pH 7.5, 37°C
0.018
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
0.02
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
0.022
dCTP
pH 7.5, 37°C, dCTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
0.024
dCTP
-
pH 9.0, 37°C, recombinant mutant enzyme
0.026
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.027
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.039
dCTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.045
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.052
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.075
dCTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.13
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.65
dCTP
-
pH and temperature not specified in the publication
0.73
dCTP
-
template base: dX, pH 7.5, 37°C
0.773
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.83
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.94
dCTP
-
template base: dT, pH 7.5, 37°C
1
dCTP
-
template base: dA, pH 7.5, 37°C
1.2
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
1.3
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
1.8
dCTP
-
template base: dC, pH 7.5, 37°C
2
dCTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
2.4
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
2.5
dCTP
-
pH and temperature not specified in the publication
0.00118
deoxynucleoside triphosphate
-
DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C
0.0014
deoxynucleoside triphosphate
wild type enzyme, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.0015
deoxynucleoside triphosphate
mutant enzyme R821A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.002
deoxynucleoside triphosphate
mutant enzyme Y824A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.0026
deoxynucleoside triphosphate
mutant enzyme R823A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.003
deoxynucleoside triphosphate
pH 8.6, 75°C, mM of each nucleotide in an equimolar mixture of the four nucleotides, mutant enzyme H633R
0.003
deoxynucleoside triphosphate
pH 8.6, 75°C, mM of each nucleotide in an equimolar mixture of the four nucleotides, wild-type enzyme
0.00305
deoxynucleoside triphosphate
-
DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C
0.0034
deoxynucleoside triphosphate
mutant enzyme R822A/Y824A in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.0037
deoxynucleoside triphosphate
mutant enzyme R822A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C
0.17
deoxynucleoside triphosphate
-
pH 7.5, 75°C, KM-value of each nucleotide in an equimolar mixture of the four nucleotides, fusion protein of the Sso7d protein to the C-terminus of Tpa DNA polymerase
0.64
deoxynucleoside triphosphate
-
pH 7.5, 75°C, KM-value of each nucleotide in an equimolar mixture of the four nucleotides, unmodified Tpa DNA polymerase
0.263
dGMP:dG
-
37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G
-
0.3511
dGMP:dG
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template G
-
0.00022
dGTP
-
pH 8.0, 25°C
0.0053
dGTP
-
pH and temperature not specified in the publication
0.012
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.013
dGTP
pH 7.8, 37°C, dGTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.017
dGTP
-
template base: dC, pH 7.5, 37°C
0.028
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8ocoG)CACT-5'
0.049
dGTP
pH 7.8, 37°C, dGCTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.05
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.077
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.084
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.086
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.19
dGTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.19
dGTP
-
template base: dG, pH 7.5, 37°C
0.2
dGTP
-
template base: dI, pH 7.5, 37°C
0.25
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.31
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.43
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.47
dGTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.48
dGTP
-
template base: dX, pH 7.5, 37°C
0.58
dGTP
-
template base: dT, pH 7.5, 37°C
0.6
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.77
dGTP
-
template base: dA, pH 7.5, 37°C
1.2
dGTP
-
pH and temperature not specified in the publication
1.2
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
1.4
dGTP
-
pH and temperature not specified in the publication
1.6
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
2.3
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
2.3
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
2.5
dGTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.00000066
DNAn
-
0.0000032
DNAn
-
pH 7.5, 75°C, template in the presence of an excess of annealed primer, fusion protein of the Sso7d protein to the C-terminus of Tpa DNA polymerase
0.0000057
DNAn
-
pH 7.5, 75°C, template in the presence of an excess of annealed primer, unmodified Tpa DNA polymerase
0.0000066
DNAn
pH 8.6, 75°C, mM of template, in the presence of an excess of annealed primer, mutant enzyme H633R
0.0000104
DNAn
pH 8.6, 75°C, mM of template, in the presence of an excess of annealed prime, wild-type enzyme
0.0013
DNAn
-
in the presence of dNTPs
0.0017
DNAn
-
in the absence of dNTPs
1.26
dTMP:dG
-
37°C, pH 7.5, wild-type enzyme, substitution opposite the template G
-
1.43
dTMP:dG
-
37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G
-
0.00035
dTTP
-
pH 8.0, 25°C
0.00132
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mn2+
0.002
dTTP
-
pH 9.0, 25°C
0.006
dTTP
pH 7.8, 37°C, dTTP insertion opposite the N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.0061
dTTP
-
template base: dA, pH 7.5, 37°C
0.0093
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
0.0103
dTTP
pH 7.5, 37°C, dTTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
0.013
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
0.01469
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mg2+
0.029
dTTP
pH 7.8, 37°C, dTTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.0314
dTTP
-
pH 8.0, 22°C, recombinant enzyme
0.11
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.14
dTTP
-
pH 9.0, 37°C, recombinant mutant enzyme
0.175
dTTP
-
wild-type enzyme, pH 7.5, 37°C, misincorporation of dTTP with G at first position
0.2
dTTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
0.211
dTTP
-
mutant L606G, pH 7.5, 37°C, misincorporation of dTTP with G at first position
0.23
dTTP
pH 8.2, 50°C, recombinant M1pol
0.28
dTTP
-
pH 8.2, 50°C, recombinant enzyme mutant K4polL329A
0.316
dTTP
-
pH 8.0, 22°C, recombinant enzyme
0.38
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.56
dTTP
-
template base: dX, pH 7.5, 37°C
0.75
dTTP
-
template base: dT, pH 7.5, 37°C
0.78
dTTP
-
template base: dG, pH 7.5, 37°C
0.82
dTTP
-
template base: dI, pH 7.5, 37°C
0.87
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.93
dTTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.94
dTTP
-
pH and temperature not specified in the publication
0.94
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.96
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.99
dTTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
1
dTTP
-
pH and temperature not specified in the publication
1.1
dTTP
-
template base: dC, pH 7.5, 37°C
1.3
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
1.6
dTTP
pH 7.5, 37°C, DNA polymerase Dpo4
1.8
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
2.1
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
2.3
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
2.7
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
3.1
dTTP
pH 7.5, 37°C, DNA polymerase Dpo1
3.7
dTTP
pH 7.5, 37°C, DNA polymerase Dpo2
3.8
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
8.9
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
38.1
dTTP
-
pH 8.0, 37°C, recombinant enzyme
47.4
dTTP
-
pH 8.0, 50°C, recombinant enzyme
0.223
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
0.232
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.279
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.287
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.403
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.0012
North-methanocarba-dATP
pH 7.5, 37°C
0.0087
North-methanocarba-dATP
pH 7.5, 37°C
additional information
additional information
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additional information
additional information
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additional information
additional information
Tequatrovirus T4
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additional information
additional information
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additional information
additional information
Salasvirus phi29
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additional information
additional information
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additional information
additional information
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steady-state kinetics
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additional information
additional information
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biphasic kinetic analysis support a universal kinetic mechanism for the bypass of DNA double lesions, binding constants of the Dpo4-DNA binary complex, overview
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additional information
additional information
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pre-steady state kinetics, and biphasic kinetics of nucleotide incorporation at 56°C
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additional information
additional information
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kinetic parameters for nucleotide incorporation by Sso pol B1 exo(-) at 55°C
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additional information
additional information
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kinetic parameters of several elementary steps for the forward polymerization reaction
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additional information
additional information
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pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
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additional information
additional information
pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
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additional information
additional information
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steady-state kinetic parameters for dCTP incorporation by Dpo4 T239W
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additional information
additional information
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steady-state kinetic parameters for next-base extension past 3-(2'-deoxy-beta-D-erythro-pentofuranosyl) pyrimido[1,2-alpha]purin-10(3H)-one
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additional information
additional information
steady-state kinetic parameters for one-base incorporation
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additional information
additional information
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steady-state kinetic parameters for one-base incorporation
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additional information
additional information
steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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additional information
additional information
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steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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additional information
additional information
steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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additional information
additional information
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steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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additional information
additional information
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steady-state kinetic parameters for the single dNTP incorporation by Dpo4 T239W
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additional information
additional information
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biphasic dissociation kinetics of the polymerase-DNA binary complex
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additional information
additional information
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in pre-steady-state kinetic measurements, when temperature is raised from 22°C to 50°C, the rate (kpol) for cognate dTTP and non-cognate dATP nucleotide incorporations increases 6 and 4fold, respectively, whereas the Kd for both nucleotide incorporations changes only slightly. Single turnover kinetics
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additional information
additional information
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kinetic constants for DNA-dependent and RNA-dependent DNA polymerization Michaelis-Menten mechanism and kinetic model of the mutant enzyme, overview
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additional information
additional information
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kinetics of nucleotide binding and incorporation, detailed overview. Weak binding is followed by a fast conformational change leading to much tighter binding, which is then followed by the chemical reaction, fast release of diphosphate, structure-function modeling
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additional information
additional information
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kinetics of nucleotide binding and incorporation, detailed overview. Weak binding is followed by a fast conformational change leading to much tighter binding, which is then followed by the chemical reaction, following the chemistry step, the enzyme shows fast release of diphosphate and then translocates to allow the binding of the next nucleotide, structure-function modeling. single turnover analysis shows that that the rate of the chemical reaction and the rate of diphosphate release are coincident
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additional information
additional information
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Michaelis-Menten kinetics and single nucleotide kinetics of wild-type and mutat enzymes, overview
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additional information
additional information
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the reaction rate of K4polL329A exhibits a saturated profile of the Michaelis-Menten kinetics for dTTP concentrations but a substrate inhibition profile for poly(rA)-p(dT)45 concentrations
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additional information
additional information
the reaction rate of M1pol exhibits a saturated profile of the Michaelis-Menten kinetics for dTTP concentrations but a substrate inhibition profile for poly(rA)-p(dT)45 concentrations
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additional information
additional information
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transient state kinetic analyses of DNA polymerase beta, stopped flow absorbance assay development and kinetic modeling and simulations, overview. Pre-steady-state kinetic experiments measuring single nucleotide incorporation catalyzed by Pol beta are performed at 37°C, pH 7.1, over a range of both [Mg2+] and [Mg·dATP2?]. Rapid quench kinetics. Kinetics of nucleotide-induced subdomain closing
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additional information
additional information
kinetic for nucleotide insertion opposite template cytosine
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additional information
additional information
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kinetic for nucleotide insertion opposite template cytosine
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additional information
additional information
kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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additional information
additional information
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kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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additional information
additional information
pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4
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additional information
additional information
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pH 7.5, 50°C, Km is 0.71 mM for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine
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additional information
additional information
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steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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additional information
additional information
steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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additional information
additional information
steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4
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additional information
additional information
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steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT
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additional information
additional information
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kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts
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additional information
additional information
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kinetic study of dNTP primer-extension opposite a benzo[a]pyrene-N2-dG-adduct with four DNA polymerases, including Sulfolobus solfataricus Dpo4 and Sulfolobus acidocaldarius Dbh. Vmax/Km is similar for correct dCTP insertion with Dpo4 and Dbh. Compared to Dpo4, Dbh misinsertion is slower for dATP (about 20fold), dGTP (about 110fold) and dTTP (about 6fold), due to decreases in Vmax. These findings provide support that Dbh is in the same Y-Family DNA polymerase class as eukaryotic DNA polymerase kappa and bacterial DNA polymerase IV, which accurately bypass N2-dG adducts
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