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0.29 - 0.34
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
10.4
5-ethynyl-dCTP
-
pH and temperature not specified in the publication
-
4.6
5-ethynyl-dUTP
-
pH and temperature not specified in the publication
-
0.02 - 0.33
5-Methyl-dCTP
7.5
5-phenyl-dCTP
-
pH and temperature not specified in the publication
-
9.3
5-phenyl-dUTP
-
pH and temperature not specified in the publication
-
15.4
5-vinyl-dCTP
-
pH and temperature not specified in the publication
-
17.7
5-vinyl-dUTP
-
pH and temperature not specified in the publication
-
0.068 - 0.405
7-deaza-2'-deoxyadenosine 5'-triphosphate
12.1
7-deaza-dGTP
-
pH and temperature not specified in the publication
6.3
7-ethynyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
17.5
7-ethynyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
5.5
7-methyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
12.7
7-methyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
13.5
7-phenyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
13.7
7-phenyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
7.1
7-vinyl-7-deaza-dATP
-
pH and temperature not specified in the publication
-
13.8
7-vinyl-7-deaza-dGTP
-
pH and temperature not specified in the publication
-
0.056
ATP
-
polymerase activity, D190A mutant, pH 7.6, 37°C
1.16
Cy3-dATP
-
pH 8.0, 25°C
-
1.75
Cy3-dCTP
-
pH 8.0, 25°C
-
1.64
Cy3-dGTP
-
pH 8.0, 25°C
-
1.57
Cy3-dUTP
-
pH 8.0, 25°C
-
6
dADP
pH and temperature not specified in the publication
0.0667 - 116
deoxynucleoside triphosphate
0.05
dPTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.012 - 0.15
N1-methyl-2'-deoxyadenosine 5'-triphosphate
0.014 - 0.08
North-methanocarba-dATP
0.000042 - 0.011
primed M13
-
0.0088
South-methanocarba-dATP
pH 7.5, 37°C
6.6
TTP
-
pH and temperature not specified in the publication
additional information
additional information
-
0.29
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.34
2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.03
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.04
2-thio-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.02
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.33
5-Methyl-dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.068
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.085
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
0.2
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.265
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.405
7-deaza-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.000016
dATP
pH 7.5, 37°C, DNA polymerase Dpo3
0.000083
dATP
pH 7.8, 37°C, incorporation of dATP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.0002
dATP
pH 7.8, 37°C, dATP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.00024
dATP
pH 7.8, 37°C, dATP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
0.00025
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3'
0.00029
dATP
pH 7.8, 37°C, dATP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
0.0012
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0016
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0017
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0018
dATP
pH 7.5, 37°C, DNA polymerase Dpo1
0.0025
dATP
pH 7.8, 37°C, incorporation of dATP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8oxoG)CACT-5'
0.0027
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0028
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0029
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0036
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0053
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0057
dATP
-
template base: dG, pH 7.5, 37°C
0.0068
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0098
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.011
dATP
-
template base: dC, pH 7.5, 37°C
0.012
dATP
-
template base: dI, pH 7.5, 37°C
0.02
dATP
-
template base: dX, pH 7.5, 37°C
0.021
dATP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.022
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409I
0.022
dATP
-
template base: dA, pH 7.5, 37°C
0.028
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409M
0.029
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410V
0.034
dATP
-
55°C, pH not specified in the publication, mutant enzyme L409V
0.037
dATP
-
55°C, pH not specified in the publication, mutant enzyme A408S
0.043
dATP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.045
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.063
dATP
pH 7.5, 60°C, incorporation opposite an abasic site
0.073
dATP
pH 7.5, 37°C, DNA polymerase Dpo4
0.185
dATP
-
pH 8.0, 37°C, recombinant enzyme
0.22
dATP
-
55°C, pH not specified in the publication, wild-type enzyme
0.23
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.24
dATP
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.31
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410L
0.32
dATP
-
55°C, pH not specified in the publication, mutant enzyme Y410I
0.36
dATP
-
template base: dT, pH 7.5, 37°C
0.38
dATP
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.4
dATP
-
pH 8.0, 50°C, recombinant enzyme
0.96
dATP
-
pH 9.0, 37°C, recombinant mutant enzyme
1.69
dATP
-
pH 8.0, 25°C
8
dATP
-
pH and temperature not specified in the publication
0.000053
dCTP
pH 7.5, 37°C, DNA polymerase Dpo2
0.0001
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.00024
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCGT-G-TCAATCCTTACGAGCATCGCCCCC-3'
0.00027
dCTP
pH 7.8, 37°C, dCTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.0003
dCTP
pH 7.8, 37°C, dCTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3'
0.00053
dCTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.00053
dCTP
pH 7.8, 37°C, dCTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCGT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-TCAATCCTTACGAGCATCGCCCCC-3'
0.0008
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0021
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.0022
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.005
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8oxoG)CACT-5'
0.0054
dCTP
-
template base: dA, pH 7.5, 37°C
0.0055
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0057
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0062
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.011
dCTP
-
template base: dC, pH 7.5, 37°C
0.011
dCTP
-
template base: dX, pH 7.5, 37°C
0.013
dCTP
pH 7.8, 37°C, incorporation of dCTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.015
dCTP
-
template base: dT, pH 7.5, 37°C
0.019
dCTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
0.02
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with O6-methylguanine as template
0.022
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.027
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.038
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
0.042
dCTP
pH 7.5, 37°C, dCTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
0.044
dCTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
0.056
dCTP
pH 7.5, 37°C, dCTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
0.065
dCTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.092
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.23
dCTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.28
dCTP
pH 7.4, 37°C, steady-state kinetics for single nucleotide primer extension with guanine as template
0.34
dCTP
37°C, pH 7.4, mutant enzyme T239W
0.38
dCTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.41
dCTP
-
template base: dI, pH 7.5, 37°C
0.47
dCTP
37°C, pH 7.4, mutant enzyme N188W
0.89
dCTP
-
pH 9.0, 37°C, recombinant mutant enzyme
0.93
dCTP
-
template base: dG, pH 7.5, 37°C
1.75
dCTP
-
pH 8.0, 25°C
6.1
dCTP
-
pH and temperature not specified in the publication
12
dCTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.0667
deoxynucleoside triphosphate
-
wild-type enzyme, pH 7.5, 60°C
0.0667
deoxynucleoside triphosphate
-
wild-type enzyme, pH 7.5, 60°C
0.15
deoxynucleoside triphosphate
-
G418K/E507Q mutant, pH 7.5, 60°C
0.15
deoxynucleoside triphosphate
-
G418K/E507Q mutant, pH 7.5, 60°C
1.05
deoxynucleoside triphosphate
-
equimolar mixture of all nucleotides, pH 7.5, 70°C
2.5 - 4
deoxynucleoside triphosphate
-
pH 7.6, 30°C
3.9
deoxynucleoside triphosphate
pH 8.6, 75°C, mM of each nucleotide in an equimolar mixture of the four nucleotides, wild-type enzyme
4.2
deoxynucleoside triphosphate
pH 8.6, 75°C, mM of each nucleotide in an equimolar mixture of the four nucleotides, mutant enzyme H633R
92
deoxynucleoside triphosphate
-
pH 7.5, 75°C, turnover number of each nucleotide in an equimolar mixture of the four nucleotides, unmodified Tpa DNA polymerase
116
deoxynucleoside triphosphate
-
pH 7.5, 75°C, turnover number of each nucleotide in an equimolar mixture of the four nucleotides, fusion protein of the Sso7d protein to the C-terminus of Tpa DNA polymerase
0.000013
dGTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.0002
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.00032
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.00038
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (8oxoG) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(8oxoG)CACT-5'
0.00043
dGTP
pH 7.8, 37°C, dGTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3'
0.00055
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0006
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0006
dGTP
pH 7.8, 37°C, dGTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.00068
dGTP
pH 7.8, 37°C, incorporation of dGTP opposite (G) in the double stranded oligonucleotide: 5'-GGGGGAAGGATTC-3'/3'-CCCCCTTCCTAAG(G)CACT-5'
0.0016
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0016
dGTP
-
template base: dI, pH 7.5, 37°C
0.0033
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.0045
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0046
dGTP
-
template base: dA, pH 7.5, 37°C
0.0049
dGTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0052
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0063
dGTP
-
template base: dG, pH 7.5, 37°C
0.0067
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.0068
dGTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.01
dGTP
-
template base: dX, pH 7.5, 37°C
0.042
dGTP
-
template base: dT, pH 7.5, 37°C
0.043
dGTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.15
dGTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.54
dGTP
-
template base: dC, pH 7.5, 37°C
6.48
dGTP
-
pH 8.0, 25°C
13.1
dGTP
-
pH and temperature not specified in the publication
2.4
DNAn
pH 8.6, 75°C, mM of template, in the presence of an excess of annealed primer, mutant enzyme H633R
2.6
DNAn
pH 8.6, 75°C, mM of template, in the presence of an excess of annealed primer, wild-type enzyme
12.9
DNAn
-
pH 7.5, 75°C, mol of template, in the presence of an excess of annealed primer, fusion protein of the Sso7d protein to the C-terminus of Tpa DNA polymerase
13.6
DNAn
-
pH 7.5, 75°C, mol of template, in the presence of an excess of annealed primer, unmodified Tpa DNA polymerase
0.0000072
dTTP
pH 7.5, 37°C, DNA polymerase Dpo2
0.00011
dTTP
pH 7.5, 37°C, DNA polymerase Dpo3
0.00013
dTTP
pH 7.8, 37°C, dTTP insertion opposite N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine in 5'-TCAT-(N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine)-GAATCCTTACGAGCATCGCCCCC-3'
0.00019
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.00029
dTTP
pH 7.8, 37°C, dTTP insertion opposite an unmodified deoxyguanosine in 5'-TCAT-G-GAATCCTTACGAGCATCGCCCCC-3'
0.0004
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG
0.0021
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.0023
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.003
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG
0.0046
dTTP
-
template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C
0.0047
dTTP
-
template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C
0.0096
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mn2+
0.012
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.0123
dTTP
pH 8.8, 30°C, recombinant enzyme, in presence of Mg2+
0.014
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.014
dTTP
-
template base: dI, pH 7.5, 37°C
0.017
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.017
dTTP
-
template base: dC, pH 7.5, 37°C
0.021
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.025
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG
0.025
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site
0.031
dTTP
-
template base: dT, pH 7.5, 37°C
0.034
dTTP
-
template base: dG, pH 7.5, 37°C
0.035
dTTP
-
template base: dX, pH 7.5, 37°C
0.043
dTTP
-
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G
0.063
dTTP
pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG
0.096
dTTP
-
pH 8.0, 22°C, recombinant enzyme
0.096
dTTP
pH 7.5, 37°C, DNA polymerase Dpo1
0.16
dTTP
-
template base: dA, pH 7.5, 37°C
0.28
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-butanetriol in 5'-TCTC-N6dA-butanetriol-GTTTATGGACCACC-3'
0.35
dTTP
pH 7.5, 37°C, dTTP insertion opposite the N6dA-(OH)2butyl-GSH in 5'-TCTC-N6dA-(OH)2butyl-GSH-GTTTATGGACCACC-3'
0.36
dTTP
pH 7.5, 37°C, dTTP insertion opposite an unmodified deoxyadenosine in 5'-TCTCAGTTTATGGACCACC-3'
0.48
dTTP
pH 7.5, 37°C, DNA polymerase Dpo4
0.64
dTTP
-
pH 7.5, 25°C
1
dTTP
pH 8.2, 50°C, recombinant M1pol
1.4
dTTP
-
pH 9.0, 37°C, recombinant mutant enzyme
1.7
dTTP
-
pH 8.2, 50°C, recombinant enzyme mutant K4polL329A
6.57
dTTP
-
pH 8.0, 25°C
25.8
dTTP
-
pH 8.0, 22°C, recombinant enzyme
75.6
dTTP
-
pH 8.0, 37°C, recombinant enzyme
175
dTTP
-
pH 8.0, 50°C, recombinant enzyme
0.012
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of a cis-syn thymine dimer of the template
0.028
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite an abasic site
0.063
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of a cis-syn thymine dimer of the template
0.145
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 5'-thymine of an nondamaged template
0.15
N1-methyl-2'-deoxyadenosine 5'-triphosphate
pH 7.5, 60°C, incorporation opposite the 3'-thymine of an nondamaged template
0.014
North-methanocarba-dATP
pH 7.5, 37°C
0.08
North-methanocarba-dATP
pH 7.5, 37°C
4.17
Nucleotide
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pol II
0.000042
primed M13
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exonuclease activity, D190A mutant, pH 7.6, 37°C
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0.011
primed M13
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exonuclease activity, wild-type, pH 7.6, 37°C
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DNA polymerase IV (Dpo4) shows 90-fold higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The catalytic efficiency for these events (with dCTP or C) is similar for G and 8-oxoG templates. Extension beyond an 8-oxoG:C pair is similar to G:C and faster than for an 8-oxoG:A pair, in contrast to other polymerases. dCTP insertion opposite 8-oxoG was lower than for opposite guanine
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DNA polymerase IV (Dpo4) shows 90-fold higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The catalytic efficiency for these events (with dCTP or C) is similar for G and 8-oxoG templates. Extension beyond an 8-oxoG:C pair is similar to G:C and faster than for an 8-oxoG:A pair, in contrast to other polymerases. dCTP insertion opposite 8-oxoG was lower than for opposite guanine
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pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
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pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme
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steady-state kinetic parameters for dCTP incorporation by Dpo4 T239W
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steady-state kinetic parameters for one-base incorporation
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steady-state kinetic parameters for one-base incorporation
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steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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steady-state kinetic parameters for one-base incorporation opposite G and N2-alkyl G adducts by Dpo4. Steady-state kinetic parameters for next base extension from G (or N2-alkyl G):C (or T) template primer termini by Dpo4
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steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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steady-state kinetic parameters for single-base extension reactions by Dpo4 in the presence of either Ca2+ or Mg2+
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steady-state kinetic parameters for the single dNTP incorporation by Dpo4 T239W
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the fidelity of nucleotide incorporation by Dpo3 from the polymerase active site alone is 1000-10000 at 37°C. The functional exonuclease proofreading active site will increase fidelity by at least 100
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the fidelity of nucleotide incorporation by Dpo3 from the polymerase active site alone is 1000-10000 at 37°C. The functional exonuclease proofreading active site will increase fidelity by at least 100
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Tpa-S DNA polymerase (the fusion of the Sso7d protein to the C-terminus of Tpa DNA polymerase) has nearly an identical turnover rate for DNA with the unmodified Tpa DNA polymerase. However, Tpa-S DNA polymerase has a somewhat higher (about 1.26fold) turnover rate in nucleotide incorporation than Tpa DNA polymerase
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kinetic for nucleotide insertion opposite template cytosine
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kinetic for nucleotide insertion opposite template cytosine
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kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene
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pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4
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pH 7.5, 50°C, kcat is 0.16/s for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine
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steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini
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steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4
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steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT
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the average speed of utilization of dNTPs by the Taq polymerase is kcat: 47/s
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