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BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Gowda, A.S.; Krishnegowda, G.; Suo, Z.; Amin, S.; Spratt, T.E.
    Low fidelity bypass of O(2)-(3-pyridyl)-4-oxobutylthymine, the most persistent bulky adduct produced by the tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone by model DNA polymerases (2012), Chem. Res. Toxicol., 25, 1195-1202.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the rate of insertion of dNTPs opposite and extension past both O2-[4-(3-pyridyl)-4-oxobut-1-yl]-thymidine and O2-methylthymidine is measured. The size of the alkyl chain only marginally affects the reactivity and the specificity of adduct bypass is very low. Dpo4 catalyzes the incorporation opposite and extension past the adducts approximately 1000fold more slowly than undamaged DNA. dA is the preferred base pair partner for O2-[4-(3-pyridyl)-4-oxobut-1-yl]-thymidine and dT is the preferred base pair partner for O2-methylthymidine Saccharolobus solfataricus diphosphate + DNAn+1
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Synonyms

Synonyms Comment Organism
DNA polymerase IV
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Saccharolobus solfataricus
Dpo4
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Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information steady-state parameters for the enzyme catalyzed insertion opposite and extension past O2-alkyl-dT Saccharolobus solfataricus