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Literature summary for 2.7.7.7 extracted from

  • Bebenek, K.; Garcia-Diaz, M.; Patishall, S.R.; Kunkel, T.A.
    Biochemical properties of Saccharomyces cerevisiae DNA polymerase IV (2005), J. Biol. Chem., 280, 20051-20058.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the gap-filling activity of pol IV is not enhanced by a 5'-phosphate on the downstream primer but is stimulated by a 5'-terminal synthetic abasic site Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
dATP
-
Saccharomyces cerevisiae
0.024
-
dCTP
-
Saccharomyces cerevisiae
0.139
-
rCTP
-
Saccharomyces cerevisiae
0.189
-
rATP
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharomyces cerevisiae role in non-homologous end joining of double strand breaks, perhaps including those with damaged ends, possible role for pol IV in base excision repair diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + DNAn
-
Saccharomyces cerevisiae ?
-
?
dCTP + DNAn
-
Saccharomyces cerevisiae ?
-
?
deoxynucleoside triphosphate + DNAn role in non-homologous end joining of double strand breaks, perhaps including those with damaged ends, possible role for pol IV in base excision repair Saccharomyces cerevisiae diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme has intrinsic 5'-2-deoxyribose-5-phosphate lyase activity. Pol IV has low processivity and can fill short gaps in DNA. The gap-filling activity of pol IV is not enhanced by a 5'-phosphate on the downstream primer but is stimulated by a 5'-terminal synthetic abasic site. Pol IV incorporates rNTPs into DNA with high efficiency relative to dNTPs. Pol IV is highly inaccurate, with an unusual error specificity indicating the ability to extend primer termini with limited homology Saccharomyces cerevisiae diphosphate + DNAn+1
-
?
rATP + DNAn
-
Saccharomyces cerevisiae ?
-
?
rCTP + DNAn
-
Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
Saccharomyces cerevisiae
Pol IV
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
rATP
-
Saccharomyces cerevisiae
0.09
-
rCTP
-
Saccharomyces cerevisiae
0.14
-
dATP
-
Saccharomyces cerevisiae
0.16
-
dCTP
-
Saccharomyces cerevisiae