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Literature summary for 2.7.7.7 extracted from

  • Zhang, H.; Bren, U.; Kozekov, I.D.; Rizzo, C.J.; Stec, D.F.; Guengerich, F.P.
    Steric and electrostatic effects at the C2 atom substituent influence replication and miscoding of the DNA deamination product deoxyxanthosine and analogs by DNA polymerases (2009), J. Mol. Biol., 392, 251-269.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
dCTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.0061
-
dTTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.0068
-
dCTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.011
-
dATP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.017
-
dGTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.017
-
dCTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.19
-
dGTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.2
-
dTTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.2
-
dATP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.2
-
dGTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.48
-
dGTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.49
-
dATP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.56
-
dTTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.58
-
dGTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.72
-
dATP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.73
-
dCTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.75
-
dTTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.77
-
dGTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.78
-
dTTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.82
-
dTTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.84
-
dATP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.94
-
dCTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.99
-
dTTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
1
-
dCTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
1.1
-
dTTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
1.8
-
dCTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
1.9
-
dATP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
2
-
dCTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + DNAn
-
Saccharolobus solfataricus ?
-
?
dATP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?
dCTP + DNAn
-
Saccharolobus solfataricus ?
-
?
dCTP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?
deoxynucleoside triphosphate + DNAn removal of the 2-amino group from the template dG (i.e. deoxyinosine) has little impact on the catalytic efficiency of either polymerase, although the misincorporation frequency is increased by an order of magnitude. Deoxyxanthosine is highly miscoding with both polymerases, and incorporation of several bases is observed. The addition of bromine or oxygen at C2 lowers the Tm further, strongly inhibits and increases the frequency of misincorporation Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn removal of the 2-amino group from the template dG (i.e. deoxyinosine) has little impact on the catalytic efficiency of either polymerase, although the misincorporation frequency is increased by an order of magnitude. Deoxyxanthosine is highly miscoding with both polymerases, and incorporation of several bases is observed. The addition of bromine or oxygen at C2 lowers the Tm further, strongly inhibits and increases the frequency of misincorporation Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
dGTP + DNAn
-
Saccharolobus solfataricus ?
-
?
dGTP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?
dITP + DNAn
-
Saccharolobus solfataricus ?
-
?
dTTP + DNAn
-
Saccharolobus solfataricus ?
-
?
dTTP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?

Synonyms

Synonyms Comment Organism
Dpo4
-
Saccharolobus solfataricus
Pol IV
-
Saccharolobus solfataricus
translesion DNA polymerase
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0016
-
dGTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.0046
-
dTTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.0046
-
dGTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.0047
-
dTTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.0054
-
dCTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.0057
-
dATP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.0063
-
dGTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.01
-
dGTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.011
-
dATP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.011
-
dCTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.011
-
dCTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.012
-
dATP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.014
-
dTTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.015
-
dCTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.017
-
dTTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.019
-
dCTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.02
-
dATP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.022
-
dATP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.031
-
dTTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.034
-
dTTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.035
-
dTTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.042
-
dGTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.044
-
dCTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.16
-
dTTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.36
-
dATP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.41
-
dCTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.54
-
dGTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.93
-
dCTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0015
-
dTTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.0046
-
dTTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.0054
-
dCTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.0058
-
dATP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.006
-
dGTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.0061
-
dCTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
0.008
-
dGTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.0095
-
dCTP template base: 2-bromo-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.012
-
dATP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.015
-
dCTP template base: dX , pH 7.5, 37°C Saccharolobus solfataricus
0.016
-
dCTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.017
-
dTTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.021
-
dGTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.024
-
dTTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
0.026
-
dATP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
0.028
-
dATP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.033
-
dGTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.041
-
dTTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
0.044
-
dTTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus
0.06
-
dATP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
0.063
-
dTTP template base: dX, pH 7.5, 37°C Saccharolobus solfataricus
0.072
-
dGTP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
6.5
-
dCTP template base: 2-fluoro-2'-deoxyinosine, pH 7.5, 37°C Saccharolobus solfataricus
26
-
dTTP template base: dA, pH 7.5, 37°C Saccharolobus solfataricus
32
-
dGTP template base: dC, pH 7.5, 37°C Saccharolobus solfataricus
33
-
dATP template base: dT, pH 7.5, 37°C Saccharolobus solfataricus
34
-
dCTP template base: dI, pH 7.5, 37°C Saccharolobus solfataricus
370
-
dCTP template base: dG, pH 7.5, 37°C Saccharolobus solfataricus