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Literature summary for 2.7.7.7 extracted from

  • Nishida, M.; Hada, T.; Kuramochi, K.; Yoshida, H.; Yonezawa, Y.; Kuriyama, I.; Sugawara, F.; Yoshida, H.; Mizushina, Y.
    Diallyl sulfides: Selective inhibitors of family X DNA polymerases from garlic (Allium sativum L.) (2008), Food Chem., 108, 551-560.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Allicin
-
Homo sapiens
Allicin
-
Rattus norvegicus
alliin
-
Homo sapiens
alliin
-
Rattus norvegicus
diallyl pentasulfide
-
Homo sapiens
diallyl pentasulfide
-
Rattus norvegicus
diallyl tetrasulfide
-
Homo sapiens
diallyl tetrasulfide
-
Rattus norvegicus
diallyl trisulfide
-
Homo sapiens
diallyl trisulfide
-
Rattus norvegicus
additional information DNA polymerase alpha is not inhibited by allicin, alliin, diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Bos taurus
additional information DNA polymerase isozymes I and II are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Brassica oleracea
additional information DNA polymerase isozymes alpha, delta and epsilon are not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Drosophila melanogaster
additional information DNA polymerase I is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Escherichia coli
additional information DNA polymerase lambda is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor Homo sapiens
additional information DNA polymerase delta is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Oncorhynchus masou
additional information DNA polymerase beta is strongly inhibited by an extract from Allium sativum consisting of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide, competitive inhibitor Rattus norvegicus
additional information DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Tequatrovirus T4
additional information DNA polymerase is not inhibited by diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00118
-
deoxynucleoside triphosphate DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens
0.00305
-
deoxynucleoside triphosphate DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Brassica oleracea
-
-
-
Drosophila melanogaster
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Oncorhynchus masou
-
-
-
Rattus norvegicus
-
-
-
Tequatrovirus T4
-
-
-
Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Drosophila melanogaster diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Thermus aquaticus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Homo sapiens diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Rattus norvegicus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Bos taurus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Tequatrovirus T4 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Brassica oleracea diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Oncorhynchus masou diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase
-
Drosophila melanogaster
DNA polymerase
-
Thermus aquaticus
DNA polymerase
-
Escherichia coli
DNA polymerase
-
Tequatrovirus T4
DNA polymerase
-
Brassica oleracea
DNA polymerase alpha
-
Bos taurus
DNA polymerase beta
-
Rattus norvegicus
DNA polymerase delta
-
Oncorhynchus masou
DNA polymerase lambda
-
Homo sapiens
Klenow fragment
-
Escherichia coli
Pol
-
Thermus aquaticus
Pol
-
Tequatrovirus T4
pol alpha
-
Bos taurus
pol alpha isozyme Drosophila melanogaster
pol beta
-
Rattus norvegicus
pol delta
-
Oncorhynchus masou
pol delta isozyme Drosophila melanogaster
POl epsilon isozyme Drosophila melanogaster
Pol I isozyme Escherichia coli
Pol I isozyme Brassica oleracea
Pol II isozyme Brassica oleracea
Pol lambda X family DNA polymerase Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information Allium sativum extract has a KI value of 0.00368 mM using DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus
additional information
-
additional information Allium sativum extract has an KI value of 0.0112 mM using DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
Allium sativum extract has an IC50 of 0.0345 mM, using DNA polymerase lambda in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens additional information
additional information
-
Allium sativum extract has an IC50 value of 0.0097 mM using DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus additional information
0.115
-
DNA polymerase beta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Rattus norvegicus diallyl trisulfide
0.146
-
DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, and 0.1 mM EDTA, at 37°C Homo sapiens diallyl trisulfide