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12 - 3869
acetyl phosphate
65.4
Isobutyrate
-
at pH 5.7 and 37°C
80.2
valerate
-
at pH 5.7 and 37°C
17
acetate
mutant enzyme G331Q/I332M, at pH 5.5 and 45°C
80
acetate
pH 7.5, 30°C, recombinant His-tagged isozyme AckA2
177
acetate
-
at pH 5.7 and 37°C
190
acetate
-
mutant E97D, pH 7.5, 37°C
195
acetate
pH 7.5, 10°C, recombinant enzyme
233.3
acetate
pH 8.0, 30°C
245
acetate
pH 7.5, 15°C, recombinant enzyme
280
acetate
-
pH 7.0, 36°C
300
acetate
pH 7.5, 20°C, recombinant enzyme
318
acetate
-
acetate kinase EutQ, at 40°C, pH not specified in the publication
385
acetate
pH 7.5, 25°C, recombinant enzyme
545
acetate
-
acetate kinase EutP, at 40°C, pH not specified in the publication
560
acetate
pH 7.5, 10°C, recombinant enzyme
760
acetate
pH 7.5, 15°C, recombinant enzyme
761
acetate
pH 7.5, 30°C, recombinant His-tagged isozyme AckA1
789
acetate
wild type enzyme, at pH 5.5 and 45°C
1042
acetate
-
wild-type, pH 7.5, 37°C
1165
acetate
pH 7.5, 20°C, recombinant enzyme
1180
acetate
pH 7.5, 37°C
1420
acetate
pH 7.5, 25°C, recombinant enzyme
12
acetyl phosphate
-
mutant F179A, pH 7.4
12
acetyl phosphate
mutant enzyme G331Q/I332M, at pH 7.0 and 37°C
93
acetyl phosphate
pH 7.5, 30°C, recombinant His-tagged isozyme AckA2
105
acetyl phosphate
at pH 8.0 and 37°C
316.7
acetyl phosphate
pH 8.0, 30°C
700
acetyl phosphate
-
pH 7.0, 36°C
875
acetyl phosphate
-
pH 7.4, 25°C
875
acetyl phosphate
pH 7.5, 10°C, recombinant enzyme
890
acetyl phosphate
pH 7.5, 10°C, recombinant enzyme
1039
acetyl phosphate
-
mutant L122A, pH 7.4
1105
acetyl phosphate
pH 7.5, 30°C, recombinant His-tagged isozyme AckA1
1340
acetyl phosphate
pH 7.5, 15°C, recombinant enzyme
1550
acetyl phosphate
pH 7.5, 15°C, recombinant enzyme
1550
acetyl phosphate
wild type enzyme, at pH 7.0 and 37°C
1700
acetyl phosphate
pH 7.5, 20°C, recombinant enzyme
1764
acetyl phosphate
pH 7.5, 37°C
2100
acetyl phosphate
pH 7.5, 25°C, recombinant enzyme
2150
acetyl phosphate
pH 7.5, 20°C, recombinant enzyme
2396
acetyl phosphate
-
mutant P232A, pH 7.4
2572
acetyl phosphate
-
mutant V93G, pH 7.4
2680
acetyl phosphate
-
wild-type, pH 7.4
3167
acetyl phosphate
pH 8.0, 30°C
3600
acetyl phosphate
pH 7.5, 25°C, recombinant enzyme
3869
acetyl phosphate
-
mutant V93A, pH 7.4
0.42
ADP
mutant R241L, pH 7.4
2.63
ADP
R91A/Q43W, double mutant
3.5
ADP
mutant R91A, pH 7.4
4.5
ADP
mutant R241A, pH 7.4
4.5
ADP
mutant R241K, pH 7.4
7.7
ADP
mutant R91L, pH 7.4
10.5
ADP
R241K/Q43W, double mutant
12
ADP
mutant enzyme G331Q/I332M, at pH 7.0 and 37°C
14.6
ADP
R241A/Q43W, double mutant
23
ADP
mutant R91K, pH 7.4
25.7
ADP
R91K/Q43W, double mutant
177
ADP
-
pH 7.4, 25°C, 0.5 M NaCl
318
ADP
-
pH 7.4, 25°C, 0.5 M NaCl
884
ADP
pH 7.5, 10°C, recombinant enzyme
892
ADP
pH 7.5, 10°C, recombinant enzyme
1260
ADP
wild-type, pH 7.4
1350
ADP
pH 7.5, 15°C, recombinant enzyme
1487
ADP
at pH 8.0 and 37°C
1565
ADP
pH 7.5, 15°C, recombinant enzyme
1700
ADP
pH 7.5, 20°C, recombinant enzyme
1750
ADP
co-substrate: acetate, pH 7.5, 37°C
1915
ADP
wild type enzyme, at pH 7.0 and 37°C
2135
ADP
pH 7.5, 25°C, recombinant enzyme
2178
ADP
pH 7.5, 20°C, recombinant enzyme
3515
ADP
pH 7.5, 25°C, recombinant enzyme
0.11
ATP
mutant R91A, pH 7.0
0.22
ATP
mutant R91L, pH 7.0
0.34
ATP
pH 6.5, 30°C, recombinant mutant N213T/G332D/E336L/T385N
0.4
ATP
R241K/Q43W, double mutant
0.68
ATP
mutant R241A, pH 7.0
1.3
ATP
mutant R241K, pH 7.0
1.38
ATP
mutant R241L, pH 7.0
1.83
ATP
R241A/Q43W, double mutant
2.07
ATP
R91A/Q43W, double mutant
2.2
ATP
-
mutant F179A, pH 7.0
3.7
ATP
mutant R91K, pH 7.0
6.8
ATP
37°C, pH 7.0-7.5, mutant enzyme G239S
9.9
ATP
pH 6.5, 30°C, recombinant mutant G332D
10.5
ATP
pH 6.5, 30°C, recombinant mutant I334M
11.8
ATP
R91K/Q43W, double mutant
13
ATP
37°C, pH 7.0-7.5, mutant enzyme N211S
14
ATP
mutant enzyme G331Q/I332M, at pH 5.5 and 45°C
16
ATP
37°C, pH 7.0-7.5, mutant enzyme G331Q
16.3
ATP
pH 6.5, 30°C, recombinant mutant N213T
32
ATP
37°C, pH 7.0-7.5, mutant enzyme N211T
41
ATP
pH 6.5, 30°C, recombinant mutant G333Q
77
ATP
37°C, pH 7.0-7.5, mutant enzyme G239A
88
ATP
37°C, pH 7.0-7.5, mutant enzyme G331A
132
ATP
-
mutant P232A, pH 7.0
150
ATP
-
mutant L122A, pH 7.0
205
ATP
pH 7.5, 10°C, recombinant enzyme
235
ATP
pH 7.5, 15°C, recombinant enzyme
250
ATP
-
with acetate as cosubstrate, at pH 5.7 and 37°C
305
ATP
pH 7.5, 20°C, recombinant enzyme
347
ATP
-
mutant V93G, pH 7.0
350
ATP
pH 7.5, 25°C, recombinant enzyme
374
ATP
37°C, pH 7.0-7.5, mutant enzyme N211A
395
ATP
pH 6.5, 30°C, recombinant mutant N337E
434
ATP
-
acetate kinase EutQ, at 40°C, pH not specified in the publication
565
ATP
pH 7.5, 10°C, recombinant enzyme
657
ATP
-
acetate kinase EutP, at 40°C, pH not specified in the publication
711
ATP
wild type enzyme, at pH 5.5 and 45°C
715
ATP
37°C, pH 7.0-7.5, wild-type enzyme
745
ATP
pH 7.5, 15°C, recombinant enzyme
844
ATP
-
mutant V93A, pH 7.0
870
ATP
co-substrate: formate, pH 7.5, 37°C
900
ATP
co-substrate: propionate, pH 7.5, 37°C
908
ATP
pH 6.5, 30°C, recombinant wild-type enzyme
913
ATP
wild-type, pH 7.0
1055
ATP
-
wild-type, pH 7.0
1130
ATP
pH 7.5, 20°C, recombinant enzyme
1170
ATP
co-substrate: acetate, pH 7.5, 37°C
1500
ATP
pH 7.5, 25°C, recombinant enzyme
0.18
Butyrate
-
wild-type, pH 7.0
42.4
Butyrate
-
mutant V93A, pH 7.0
118
Butyrate
-
at pH 5.7 and 37°C
294
Butyrate
-
mutant V93G, pH 7.0
2.1
CTP
37°C, pH 7.0-7.5, mutant enzyme G331Q
17
CTP
37°C, pH 7.0-7.5, mutant enzyme G331A
460
CTP
37°C, pH 7.0-7.5, wild-type enzyme
8.6
GTP
37°C, pH 7.0-7.5, mutant enzyme G331Q
67
GTP
37°C, pH 7.0-7.5, mutant enzyme G331A
571
GTP
37°C, pH 7.0-7.5, wild-type enzyme
11
ITP
37°C, pH 7.0-7.5, mutant enzyme G331Q
84
ITP
37°C, pH 7.0-7.5, mutant enzyme G331A
742
ITP
37°C, pH 7.0-7.5, wild-type enzyme
0.37
propionate
-
mutant F179A, pH 7.0
6.7
propionate
-
mutant L122A, pH 7.0
8.5
propionate
-
mutant P232A, pH 7.0
172
propionate
-
at pH 5.7 and 37°C
218
propionate
-
wild-type, pH 7.0
840
propionate
-
mutant V93G, pH 7.0
940
propionate
pH 7.5, 37°C
1029
propionate
-
mutant V93A, pH 7.0
2.8
TTP
37°C, pH 7.0-7.5, mutant enzyme G331Q
20
TTP
37°C, pH 7.0-7.5, mutant enzyme G331A
540
TTP
37°C, pH 7.0-7.5, wild-type enzyme
2.8
UTP
37°C, pH 7.0-7.5, mutant enzyme G331Q
43
UTP
37°C, pH 7.0-7.5, mutant enzyme G331A
415
UTP
37°C, pH 7.0-7.5, wild-type enzyme