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Literature summary for 2.7.2.1 extracted from

  • Chittori, S.; Savithri, H.S.; Murthy, M.R.
    Structural and mechanistic investigations on Salmonella typhimurium acetate kinase (AckA): identification of a putative ligand binding pocket at the dimeric interface (2012), BMC Struct. Biol., 12, 24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of Salmonella typhimurium AckA unliganded (Form-I, 2.70 A resolution) and citrate-bound (Form-II, 1.90 A resolution) are reported. Unexpectedly, Form-II StAckA structure show a drastic change in the conformation of residues 230-300 compared to that of Form-I Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
2,4-diaminobutyric acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2-oxobutyrate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoglutarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
citrate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
succinate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.075
-
 ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.078
-
 GTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.088
-
 CTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.096
-
 UTP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
 ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.28
-
 acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2
-
 acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
11.2
-
 propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
13.5
-
 formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
13.5
-
 ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Vmax: 15%, compared to Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ Vmax: 100% Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ Vmax: 100% Salmonella enterica subsp. enterica serovar Typhimurium
Ni2+ Vmax: 5%, compared to Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 calculated from cDNA Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium P63411
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, 15 days, no loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 r
ATP + formate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + formyl phosphate
-
 ?
ATP + propionate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + propionyl phosphate
-
 ?
CTP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium CDP + acetyl phosphate
-
 ?
GTP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium GDP + acetyl phosphate
-
 ?
UTP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium UDP + acetyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
StAckA
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 37 optimal temperature range Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
870
-
 ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
880
-
 formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
900
-
 ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
940
-
 propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1170
-
 ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1180
-
 acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1750
-
 ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1764
-
 acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
7.2 7.5 optimal pH range Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3
-
 pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutyrate
5
-
 pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2,4-diaminobutyric acid
7
-
 pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoglutarate
63
-
 pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium citrate
75
-
 pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium succinate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
68
-
 formate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
85
-
 propionate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
985
-
 acetate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
6300
-
 acetyl phosphate pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
10240
-
 ATP co-substrate: formate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
11350
-
 ATP co-substrate: propionate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
16860
-
 ATP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
17650
-
 ADP co-substrate: acetate, pH 7.5, 37°C Salmonella enterica subsp. enterica serovar Typhimurium