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2.7.2.1: acetate kinase

This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.

Word Map on EC 2.7.2.1

Reaction

ATP
+
acetate
=
ADP
+
acetyl phosphate

Synonyms

acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.1 acetate kinase

Temperature Stability

Temperature Stability on EC 2.7.2.1 - acetate kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 80
30 - 70
35 - 60
-
84% remaining activity at 35°C, 49% at 45°C, almost zero at 60°C
50 - 70
-
with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity
55 - 100
-
almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4
60
-
10 min incubation causes a loss in activity of 50%
60 - 80
-
retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C
60 - 90
stable for 30 min at 60°C, loses activity after 30 min at 70°C, 10 min at 80°C and 5 min at 90°C