2.7.2.1: acetate kinase
This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.
Word Map on EC 2.7.2.1
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2.7.2.1
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phosphotransacetylase
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acetyl-coa
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cdc42
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methanosarcina
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thermophila
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sludge
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acetogenic
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cdc42-associated
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acetylphosphate
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formate-lyase
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non-receptor
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acetobutylicum
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substrate-level
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acetoin
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tyrobutyricum
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adp-forming
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phosphoketolase
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butyryl-coa
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embden-meyerhof-parnas
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acetate-activating
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synthesis
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industry
- 2.7.2.1
- phosphotransacetylase
- acetyl-coa
- cdc42
- methanosarcina
- thermophila
- sludge
-
acetogenic
-
cdc42-associated
- acetylphosphate
- formate-lyase
-
non-receptor
- acetobutylicum
-
substrate-level
- acetoin
- tyrobutyricum
-
adp-forming
- phosphoketolase
- butyryl-coa
-
embden-meyerhof-parnas
-
acetate-activating
- synthesis
- industry
Reaction
Synonyms
acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase
ECTree
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Temperature Stability
Temperature Stability on EC 2.7.2.1 - acetate kinase
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20 - 80
30 - 70
35 - 60
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84% remaining activity at 35°C, 49% at 45°C, almost zero at 60°C
50 - 70
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with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity
55 - 100
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almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4
60 - 80
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retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C
60 - 90
stable for 30 min at 60°C, loses activity after 30 min at 70°C, 10 min at 80°C and 5 min at 90°C
65
70
purified recombinant His-tagged enzyme, pH 7.5, 50% of maximal activity remaining after 15 min at 55°C for the free enzyme, at 65°C for the acetate-bound enzyme, and at 70°C for the ATP-bound enzyme. Free enzyme is completely stable up to 40°C, inactivation of all enzymes at 80°C
20 - 80
purified recombinant His-tagged enzyme, pH 7.5, the enzyme is completely stable up to 30°C, half-life is about 40-50°C, inactivation at 80°C
purified recombinant enzyme, pH 8.0, 50-70% activity is retained after a 3 h exposure at 30-70°C
30 - 70
50-70% activity is retained after a 3 h exposure at 30-70°C
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stable to heating for 15 min, but rapidly inactivated at higher temperatures
70
stable for 15 min, rapidly inactivated at higher temperature