Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.2.1: acetate kinase

This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.

Word Map on EC 2.7.2.1

Reaction

ATP
+
acetate
=
ADP
+
acetyl phosphate

Synonyms

acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.1 acetate kinase

Cloned

Cloned on EC 2.7.2.1 - acetate kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ack gene identified by PCR, heterologously expressed in Escherichia coli JM 109
cloned and hyper-expressed in Escherichia coli
-
DNA fragment encoding 246 bp of the acetate kinase gene of strain DSM 792 cloned by a PCR-based approach
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Rosetta-2 cells
-
expressed in Escherichia coli BL21(lambdaDE3) cells
-
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Rosetta(DE3) and BL21(DE3) cells
-
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells
expressed in Escherichia coli, reporter system used that allows cloning of promoter fragments in front of a promoterless lacZ gene and insertion in Mycoplasma pneumoniae
expressed on cell surface of Escherichia coli BL21(DE3) cells
expression in Escherichia coli
functionally expressed of the His6-tagged enzyme in Escherichia coli JM109
gene ack, DNA and amino acid sequence determination, RT-PCR analysis, transcriptional organization, phylogenetic analysis in Methylotrophic bacteria, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3)
gene ackA, recombinant expression in Escherichia coli strain BL21(DE3), co-overexpression with Escherichia coli thymidylate kinase, EC 2.7.4.9, resulting in a large number of inclusion bodies
gene ackA, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
gene ackA, recombinant His-tagged enzyme overexpression in Escherichia coli strain BL21(DE3), subcloning in Saccharomyces cerevisiae strain YPH499
gene ackA, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
gene acka1, recombinant expression of His-tagged isozyme AckA1 in Escherichia coli strain MC1061
gene acka2, recombinant expression of His-tagged isozyme AckA2 in Escherichia coli strain MC1061
heterologous expression of the His6-tagged enzyme in Escherichia coli Rosetta (DE3)
overexpressed in Escherichia coli BL21(DE3)
recombinant acetate kinase with a 6-residue N-terminal histidine tag is heterologously produced in Escherichia coli BL21(DE3)
wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b
-
wild-type and variant acetate kinases are overproduced in Escherichia coli BL21(DE3)
-