Any feedback?
Literature summary for 2.7.2.1 extracted from
- Purification and properties of acetate kinase from Acholeplasma laidlawii (1979), J. Bacteriol., 137, 764-772.
General Stability
| General Stability | Organism |
|---|---|
| almost no decrease in activity after several freezing and thawing cycles | Acholeplasma laidlawii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl phosphate | - |
Acholeplasma laidlawii |  |
| ADP | - |
Acholeplasma laidlawii | |
| HgCl2 | - |
Acholeplasma laidlawii | |
| K+ | - |
Acholeplasma laidlawii | |
| additional information | not inactivated by N-ethylmaleimide | Acholeplasma laidlawii | |
| Na+ | - |
Acholeplasma laidlawii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
acetyl phosphate | pH 7.4, 25°C | Acholeplasma laidlawii | |
| 0.2 | - |
acetyl phosphate | pH 7.4, 25°C, 0.5 M NaCl | Acholeplasma laidlawii | |
| 0.24 | - |
ADP | pH 7.4, 25°C | Acholeplasma laidlawii | |
| 0.3 | - |
ATP | pH 7.4, 25°C | Acholeplasma laidlawii | |
| 1.53 | - |
ADP | pH 7.4, 25°C, 0.5 M NaCl | Acholeplasma laidlawii | |
| 38.5 | - |
acetate | pH 7.4, 25°C | Acholeplasma laidlawii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Acholeplasma laidlawii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11900 | - |
gel filtration, Sephadex G200 | Acholeplasma laidlawii |
| 51000 | - |
2 * 51000, SDS-PAGE | Acholeplasma laidlawii |
| 64000 | - |
sucrose density gradient ultracentrifugation, 0.5 M NaCl | Acholeplasma laidlawii |
| 71000 | - |
gel filtration, Sephadex G200, 0.5 M NaCl | Acholeplasma laidlawii |
| 130000 | - |
sucrose density gradient ultracentrifugation | Acholeplasma laidlawii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Acholeplasma laidlawii | - |
ADP + acetyl phosphate | - |
r | |
| ATP + acetate | Acholeplasma laidlawii oral | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acholeplasma laidlawii | - |
oral strain | - |
| Acholeplasma laidlawii oral | - |
oral strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acholeplasma laidlawii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 205.9 | - |
- |
Acholeplasma laidlawii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, almost no decrease in activity within 1 year of storage | Acholeplasma laidlawii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Acholeplasma laidlawii | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | - |
Acholeplasma laidlawii oral | ADP + acetyl phosphate | - |
r | |
| ATP + propionate | - |
Acholeplasma laidlawii | ADP + propionyl phosphate | - |
r | |
| CTP + acetate | - |
Acholeplasma laidlawii | CDP + acetyl phosphate | - |
r | |
| CTP + acetate | - |
Acholeplasma laidlawii oral | CDP + acetyl phosphate | - |
r | |
| GTP + acetate | - |
Acholeplasma laidlawii | GDP + acetyl phosphate | - |
r | |
| GTP + acetate | - |
Acholeplasma laidlawii oral | GDP + acetyl phosphate | - |
r | |
| ITP + acetate | - |
Acholeplasma laidlawii | IDP + acetyl phosphate | - |
r | |
| ITP + acetate | - |
Acholeplasma laidlawii oral | IDP + acetyl phosphate | - |
r | |
| additional information | UTP is no phosphate donor, lactate, succinate, maleate formate and bicarbonate are not acceptors | Acholeplasma laidlawii | ? | - |
? | |
| additional information | UTP is no phosphate donor, lactate, succinate, maleate formate and bicarbonate are not acceptors | Acholeplasma laidlawii oral | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, SDS-PAGE | Acholeplasma laidlawii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 177 | - |
ADP | pH 7.4, 25°C, 0.5 M NaCl | Acholeplasma laidlawii | |
| 268 | - |
ATP | pH 7.4, 25°C | Acholeplasma laidlawii | |
| 318 | - |
ADP | pH 7.4, 25°C, 0.5 M NaCl | Acholeplasma laidlawii | |
| 875 | - |
acetyl phosphate | pH 7.4, 25°C | Acholeplasma laidlawii | |
| 1000 | - |
ADP | pH 7.4, 25°C | Acholeplasma laidlawii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
