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2.7.2.1: acetate kinase

This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.

Word Map on EC 2.7.2.1

Reaction

ATP
+
acetate
=
ADP
 +
acetyl phosphate

Synonyms

acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.1 acetate kinase

Purification

Purification on EC 2.7.2.1 - acetate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes purified
-
gel filtration
HisTrap column chromatography
purified wild-type and recombinant enzymes, R175L and R340A are unstable and cannot be purified
-
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography and anion exchange chromatography
recombinant His-tagged isozyme AckA1 from Escherichia coli strain MC1061 by nickel affinity chromatography
recombinant His-tagged isozyme AckA2 from Escherichia coli strain MC1061 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain DH5alpha by ATP affinity chromatography
Strep-Tactin Sepharose column chromatography
-
Talon resin column chromatography, MonoQ column chromatography and Superdex 200 pg gel filtration
using imidazole elution [20 mM Tris-HCl, pH 7.9, 0.5 M NaCl, 10% glycerol, 8 M urea, 1 mM PMSF (pH 7.9)] buffers containing final concentrations of imidazole (100 mM, 200 mM, and 1 M imidazole)