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Literature summary for 2.7.2.1 extracted from

  • Gorrell, A.; Lawrence, S.H.; Ferry, J.G.
    Structural and kinetic analyses of arginine residues in the active site of the acetate kinase from Methanosarcina thermophila (2005), J. Biol. Chem., 280, 10731-10742.
    View publication on PubMed
Search for more literature for 2.7.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
in presence of substrate and transition states analogues Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
R241A severe decrease in kinetic parameters Methanosarcina thermophila
R241L severe decrease in kinetic parameters Methanosarcina thermophila
R91A severe decrease in kinetic parameters Methanosarcina thermophila
R91K severe decrease in kinetic parameters Methanosarcina thermophila
R91L severe decrease in kinetic parameters Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-(gamma-thio)triphosphate
-
 Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 ATP mutant R91A, pH 7.0 Methanosarcina thermophila
0.063
-
 ADP mutant R91A, pH 7.4 Methanosarcina thermophila
0.063
-
 ADP mutant R91L, pH 7.4 Methanosarcina thermophila
0.068
-
 ATP mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
0.068
-
 ATP wild-type, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
0.076
-
 ATP mutant R91K, pH 7.0 Methanosarcina thermophila
0.08
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
0.098
-
 ADP wild-type, pH 7.4 Methanosarcina thermophila
0.145
-
 ATP mutant R91L, pH 7.0 Methanosarcina thermophila
0.168
-
 ADP mutant R91K, pH 7.4 Methanosarcina thermophila
0.21
-
 ADP mutant R241A, pH 7.4 Methanosarcina thermophila
0.264
-
 ATP mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
0.297
-
 ATP mutant R241A, pH 7.0 Methanosarcina thermophila
0.47
-
 acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
0.578
-
 ADP mutant R241K, pH 7.4 Methanosarcina thermophila
0.61
-
 acetyl phosphate mutant R91K, pH 7.4 Methanosarcina thermophila
0.84
-
 acetyl phosphate mutant R241A, pH 7.4 Methanosarcina thermophila
0.92
-
 acetyl phosphate mutant R241K, pH 7.4 Methanosarcina thermophila
1.26
-
 ADP mutant R241L, pH 7.4 Methanosarcina thermophila
1.36
-
 acetyl phosphate mutant R91A, pH 7.4 Methanosarcina thermophila
1.64
-
 acetyl phosphate mutant R241L, pH 7.4 Methanosarcina thermophila
2.3
-
 acetyl phosphate mutant R91L, pH 7.4 Methanosarcina thermophila
2.7
-
 acetate wild-type, pH 7.0 Methanosarcina thermophila
11.4
-
 ATP mutant R241K, pH 7.0 Methanosarcina thermophila
17
-
 ATP mutant R241L, pH 7.0 Methanosarcina thermophila
24
-
 acetate wild-type, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
30
-
 acetate mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
70
-
 acetate mutant R91K, pH 7.0 Methanosarcina thermophila
77
-
 acetate mutant R241K, pH 7.0 Methanosarcina thermophila
83
-
 acetate mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
250
-
 acetate mutant R91A, pH 7.0 Methanosarcina thermophila
270
-
 acetate mutant R241L, pH 7.0 Methanosarcina thermophila
420
-
 acetate mutant R91L, pH 7.0 Methanosarcina thermophila
710
-
 acetate mutant R241A, pH 7.0 Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila P38502
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + hydroxylamine
-
 Methanosarcina thermophila acetyl hydroxamate + phosphate
-
 r
ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 ATP mutant R91A, pH 7.0 Methanosarcina thermophila
0.22
-
 ATP mutant R91L, pH 7.0 Methanosarcina thermophila
0.42
-
 ADP mutant R241L, pH 7.4 Methanosarcina thermophila
0.68
-
 ATP mutant R241A, pH 7.0 Methanosarcina thermophila
1.3
-
 ATP mutant R241K, pH 7.0 Methanosarcina thermophila
1.38
-
 ATP mutant R241L, pH 7.0 Methanosarcina thermophila
3.5
-
 ADP mutant R91A, pH 7.4 Methanosarcina thermophila
3.7
-
 ATP mutant R91K, pH 7.0 Methanosarcina thermophila
4.5
-
 ADP mutant R241A, pH 7.4 Methanosarcina thermophila
4.5
-
 ADP mutant R241K, pH 7.4 Methanosarcina thermophila
7.7
-
 ADP mutant R91L, pH 7.4 Methanosarcina thermophila
23
-
 ADP mutant R91K, pH 7.4 Methanosarcina thermophila
913
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
1260
-
 ADP wild-type, pH 7.4 Methanosarcina thermophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 adenosine 5'-(gamma-thio)triphosphate pH 7.0 Methanosarcina thermophila