Crystallization (Comment) | Organism |
---|---|
in presence of substrate and transition states analogues | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
R241A | severe decrease in kinetic parameters | Methanosarcina thermophila |
R241L | severe decrease in kinetic parameters | Methanosarcina thermophila |
R91A | severe decrease in kinetic parameters | Methanosarcina thermophila |
R91K | severe decrease in kinetic parameters | Methanosarcina thermophila |
R91L | severe decrease in kinetic parameters | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-(gamma-thio)triphosphate | - |
Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
ATP | mutant R91A, pH 7.0 | Methanosarcina thermophila | |
0.063 | - |
ADP | mutant R91A, pH 7.4 | Methanosarcina thermophila | |
0.063 | - |
ADP | mutant R91L, pH 7.4 | Methanosarcina thermophila | |
0.068 | - |
ATP | mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
0.068 | - |
ATP | wild-type, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
0.076 | - |
ATP | mutant R91K, pH 7.0 | Methanosarcina thermophila | |
0.08 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
0.098 | - |
ADP | wild-type, pH 7.4 | Methanosarcina thermophila | |
0.145 | - |
ATP | mutant R91L, pH 7.0 | Methanosarcina thermophila | |
0.168 | - |
ADP | mutant R91K, pH 7.4 | Methanosarcina thermophila | |
0.21 | - |
ADP | mutant R241A, pH 7.4 | Methanosarcina thermophila | |
0.264 | - |
ATP | mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
0.297 | - |
ATP | mutant R241A, pH 7.0 | Methanosarcina thermophila | |
0.47 | - |
acetyl phosphate | wild-type, pH 7.4 | Methanosarcina thermophila | |
0.578 | - |
ADP | mutant R241K, pH 7.4 | Methanosarcina thermophila | |
0.61 | - |
acetyl phosphate | mutant R91K, pH 7.4 | Methanosarcina thermophila | |
0.84 | - |
acetyl phosphate | mutant R241A, pH 7.4 | Methanosarcina thermophila | |
0.92 | - |
acetyl phosphate | mutant R241K, pH 7.4 | Methanosarcina thermophila | |
1.26 | - |
ADP | mutant R241L, pH 7.4 | Methanosarcina thermophila | |
1.36 | - |
acetyl phosphate | mutant R91A, pH 7.4 | Methanosarcina thermophila | |
1.64 | - |
acetyl phosphate | mutant R241L, pH 7.4 | Methanosarcina thermophila | |
2.3 | - |
acetyl phosphate | mutant R91L, pH 7.4 | Methanosarcina thermophila | |
2.7 | - |
acetate | wild-type, pH 7.0 | Methanosarcina thermophila | |
11.4 | - |
ATP | mutant R241K, pH 7.0 | Methanosarcina thermophila | |
17 | - |
ATP | mutant R241L, pH 7.0 | Methanosarcina thermophila | |
24 | - |
acetate | wild-type, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
30 | - |
acetate | mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
70 | - |
acetate | mutant R91K, pH 7.0 | Methanosarcina thermophila | |
77 | - |
acetate | mutant R241K, pH 7.0 | Methanosarcina thermophila | |
83 | - |
acetate | mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl | Methanosarcina thermophila | |
250 | - |
acetate | mutant R91A, pH 7.0 | Methanosarcina thermophila | |
270 | - |
acetate | mutant R241L, pH 7.0 | Methanosarcina thermophila | |
420 | - |
acetate | mutant R91L, pH 7.0 | Methanosarcina thermophila | |
710 | - |
acetate | mutant R241A, pH 7.0 | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | P38502 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + hydroxylamine | - |
Methanosarcina thermophila | acetyl hydroxamate + phosphate | - |
r | |
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
ATP | mutant R91A, pH 7.0 | Methanosarcina thermophila | |
0.22 | - |
ATP | mutant R91L, pH 7.0 | Methanosarcina thermophila | |
0.42 | - |
ADP | mutant R241L, pH 7.4 | Methanosarcina thermophila | |
0.68 | - |
ATP | mutant R241A, pH 7.0 | Methanosarcina thermophila | |
1.3 | - |
ATP | mutant R241K, pH 7.0 | Methanosarcina thermophila | |
1.38 | - |
ATP | mutant R241L, pH 7.0 | Methanosarcina thermophila | |
3.5 | - |
ADP | mutant R91A, pH 7.4 | Methanosarcina thermophila | |
3.7 | - |
ATP | mutant R91K, pH 7.0 | Methanosarcina thermophila | |
4.5 | - |
ADP | mutant R241A, pH 7.4 | Methanosarcina thermophila | |
4.5 | - |
ADP | mutant R241K, pH 7.4 | Methanosarcina thermophila | |
7.7 | - |
ADP | mutant R91L, pH 7.4 | Methanosarcina thermophila | |
23 | - |
ADP | mutant R91K, pH 7.4 | Methanosarcina thermophila | |
913 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
1260 | - |
ADP | wild-type, pH 7.4 | Methanosarcina thermophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
adenosine 5'-(gamma-thio)triphosphate | pH 7.0 | Methanosarcina thermophila |