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2.7.1.74: deoxycytidine kinase

This is an abbreviated version!
For detailed information about deoxycytidine kinase, go to the full flat file.

Word Map on EC 2.7.1.74

Reaction

NTP
+
deoxycytidine
=
NDP
 +
dCMP

Synonyms

2'-deoxycytidine kinase, ara-C kinase, arabinofuranosylcytosine kinase, dC kinase, dCK, deoxycytidine kinase, deoxycytidine-cytidine kinase, dNTP:deoxycytidine 5'-phosphotransferase, kinase, deoxycytidine (phosphorylating), NTP:deoxycytidine 5'-phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.74 deoxycytidine kinase

Storage Stability

Storage Stability on EC 2.7.1.74 - deoxycytidine kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 months
-
-20°C, in 5 mM DTT and 50% glycerol, stable
-
-30°C, several months in the presence of 2-mercaptoethanol
-
-70°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 12 months
-
-85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months
-
4°C, at least 1 week
-
4°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 2 weeks
-
4°C, if purified in the absence of 2-mercaptoethanol it loses most of its activity within 2 days, DTT restores
-
4°C, t1/2: 7 days with and t1/2: 72 h without bovine serum albumin
-
dithiothreitol is more effective than 2-mercaptoethanol in stabilizing during storage
-
glycerol, bovine serum albumin, 2-mercaptoethanol or EDTA does not enhance storage stability
-