2.7.1.74: deoxycytidine kinase
This is an abbreviated version!
For detailed information about deoxycytidine kinase, go to the full flat file.
Word Map on EC 2.7.1.74
-
2.7.1.74
-
leukemia
-
gemcitabine
-
thymidine
-
triphosphate
-
deaminase
-
purine
-
ribonucleotide
-
deoxynucleoside
-
pyrimidine
-
deoxyguanosine
-
salvage
-
myeloid
-
arabinoside
-
cytarabine
-
prodrugs
-
deoxyadenosine
-
1-beta-d-arabinofuranosylcytosine
-
deoxyribonucleoside
-
5'-nucleotidase
-
cladribine
-
cross-resistance
-
ara-ctp
-
2-chlorodeoxyadenosine
-
2',2'-difluorodeoxycytidine
-
fludarabine
-
dttp
-
antileukemic
-
ccrf-cem
-
dfdctp
-
2-chloro-2'-deoxyadenosine
-
gemcitabine-resistant
-
dado
-
deoxycytidylate
-
kinase-deficient
-
clofarabine
-
2',3'-dideoxycytidine
-
5'-phosphorylation
-
arabinosyl
-
deoxypyrimidine
-
t-lymphoblastoid
-
deoxycoformycin
-
medicine
-
2'-deoxyadenosine
-
deoxythymidine
-
l-nucleoside
-
dideoxycytidine
-
t-lymphoblasts
-
2',3'-dideoxyinosine
-
tetrahydrouridine
-
arabinosylcytosine
-
anabolites
-
pharmacology
-
analysis
- 2.7.1.74
- leukemia
- gemcitabine
- thymidine
- triphosphate
- deaminase
- purine
- ribonucleotide
- deoxynucleoside
- pyrimidine
- deoxyguanosine
-
salvage
- myeloid
- arabinoside
- cytarabine
-
prodrugs
- deoxyadenosine
- 1-beta-d-arabinofuranosylcytosine
- deoxyribonucleoside
- 5'-nucleotidase
- cladribine
-
cross-resistance
- ara-ctp
- 2-chlorodeoxyadenosine
- 2',2'-difluorodeoxycytidine
- fludarabine
- dttp
-
antileukemic
-
ccrf-cem
-
dfdctp
- 2-chloro-2'-deoxyadenosine
-
gemcitabine-resistant
-
dado
- deoxycytidylate
-
kinase-deficient
- clofarabine
- 2',3'-dideoxycytidine
-
5'-phosphorylation
-
arabinosyl
-
deoxypyrimidine
-
t-lymphoblastoid
- deoxycoformycin
- medicine
- 2'-deoxyadenosine
- deoxythymidine
-
l-nucleoside
- dideoxycytidine
- t-lymphoblasts
- 2',3'-dideoxyinosine
- tetrahydrouridine
- arabinosylcytosine
-
anabolites
- pharmacology
- analysis
Reaction
Synonyms
2'-deoxycytidine kinase, ara-C kinase, arabinofuranosylcytosine kinase, dC kinase, dCK, deoxycytidine kinase, deoxycytidine-cytidine kinase, dNTP:deoxycytidine 5'-phosphotransferase, kinase, deoxycytidine (phosphorylating), NTP:deoxycytidine 5'-phosphotransferase
ECTree
2.7.1.74 deoxycytidine kinaseAdvanced search results
results (
results (Turnover Number
Turnover Number on EC 2.7.1.74 - deoxycytidine kinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.02
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
-
pH 7.6, 37°C, recombinant enzyme
0.38
cytarabine
-
wild type enzyme, pH and temperature not specified in the publication
0.028
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.03
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.072
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.102
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.15
1-beta-D-arabinofuranosylcytosine
residues 65-79 deletion mutant with UTP as cosubstrate
0.17
1-beta-D-arabinofuranosylcytosine
wild type enzyme with UTP as cosubstrate
0.3
1-beta-D-arabinofuranosylcytosine
wild type enzyme with ATP as cosubstrate
0.41
1-beta-D-arabinofuranosylcytosine
residues 65-79 deletion mutant with ATP as cosubstrate
0.34
1-beta-D-arabinosylcytosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.79
1-beta-D-arabinosylcytosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
1.43
1-beta-D-arabinosylcytosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.31
2'-deoxyadenosine
residues 65-79 deletion mutant with UTP as cosubstrate
2.13
2'-deoxyadenosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
2.8
2'-deoxyadenosine
residues 65-79 deletion mutant with ATP as cosubstrate
4.51
2'-deoxyadenosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
5.72
2'-deoxyadenosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.033
2'-deoxycytidine
less than 0.001 mM, wild type enzyme with ATP as cosubstrate
0.04
2'-deoxycytidine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.044
2'-deoxycytidine
less than 0.001 mM, wild type enzyme with UTP as cosubstrate
0.049
2'-deoxycytidine
residues 65-79 deletion mutant with ATP as cosubstrate
0.088
2'-deoxycytidine
less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate
0.25
2'-deoxycytidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
1.8
2'-deoxycytidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.32
2'-deoxyguanosine
residues 65-79 deletion mutant with UTP as cosubstrate
1.73
2'-deoxyguanosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
2.2
2'-deoxyguanosine
residues 65-79 deletion mutant with ATP as cosubstrate
2.6
2'-deoxyguanosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
3.66
2'-deoxyguanosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.028
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.03
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
0.036
ATP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.039
ATP
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.173
ATP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.192
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.36
ATP
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.4
ATP
wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate
1.74
D-thymidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
3.2
D-thymidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
2.73
deoxyadenosine
at pH 8.0, temperature not specified in the publication
4.07
deoxyadenosine
at pH 8.0, temperature not specified in the publication
0.035
deoxycytidine
-
wild type enzyme, pH and temperature not specified in the publication
0.05
deoxycytidine
at pH 8.0, temperature not specified in the publication
0.09
deoxycytidine
at pH 8.0, temperature not specified in the publication
1.23
deoxyguanosine
at pH 8.0, temperature not specified in the publication
3.4
deoxyguanosine
at pH 8.0, temperature not specified in the publication
0.29
deoxythymidine
at pH 8.0, temperature not specified in the publication
1.08
deoxythymidine
at pH 8.0, temperature not specified in the publication
1.05
deoxyuridine
at pH 8.0, temperature not specified in the publication
1.27
deoxyuridine
at pH 8.0, temperature not specified in the publication
0.39
gemcitabine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.57
gemcitabine
-
wild type enzyme, pH and temperature not specified in the publication
2.01
gemcitabine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
2.68
gemcitabine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
1.33
L-thymidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
3.13
L-thymidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.095
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.179
troxacitabine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.192
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.524
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.031
UTP
wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate
0.041
UTP
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.042
UTP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.072
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.075
UTP
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.093
UTP
wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.102
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
0.217
UTP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.524
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
