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Literature summary for 2.7.1.74 extracted from

  • Bohman, C.; Eriksson, S.
    Deoxycytidine kinase from human leukemic spleen: preparation and characteristics of homogeneous enzyme (1988), Biochemistry, 27, 4258-4265.
    View publication on PubMed
Search for more literature for 2.7.1.74

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dCTP kinetics Homo sapiens
deoxyadenosine deoxycytidine Homo sapiens
deoxycytidine deoxyadenosine as substrate; deoxyguanosine as substrate Homo sapiens
deoxyguanosine deoxycytidine as substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens
0.0008 0.0015 deoxycytidine pH 8.0, 37°C Homo sapiens
0.02 0.04 cytosine arabinoside pH 8.0, 37°C Homo sapiens
0.12
-
 deoxyadenosine pH 8.0, 37°C Homo sapiens
0.15
-
 deoxyguanosine pH 8.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion not Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+/ATP complex is the reactive species Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Homo sapiens
61000
-
 gel filtration, glycerol gradient centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens
spleen Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell leukemic T-lymphoblasts Homo sapiens
-
leukemia cell from spleen Homo sapiens
-
additional information very low activity in normal spleens and placentas Homo sapiens
-
spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11 0.15 pH 8.0, 37°C Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 12 months Homo sapiens
4°C, concentrated enzyme solution in Tris-buffer with MgCl2 and mercaptoethanol, at least 2 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 5'-dCMP
-
 ?
dTTP + 2'-deoxycytidine
-
 Homo sapiens dTDP + 2'-deoxy-CMP
-
 ?
additional information presumably one common nucleoside acceptor site Homo sapiens ?
-
 ?
NTP + cytosine arabinoside
-
 Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + deoxyadenosine
-
 Homo sapiens NDP + dAMP
-
 ?
NTP + deoxycytidine
-
 Homo sapiens NDP + dCMP
-
 ?
NTP + deoxyguanosine
-
 Homo sapiens NDP + dGMP
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.5 plateau, higher activity in Tris than in phosphate buffer Homo sapiens