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Literature summary for 2.7.1.74 extracted from

  • Kierdaszuk, B.; Eriksson, S.
    Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergents (1990), Biochemistry, 29, 4109-4114.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CHAPS slight stimulation, deoxycytidine as substrate Homo sapiens
sodium cholate slight stimulation, deoxycytidine as substrate Homo sapiens
Zwittergent 3-14 slight stimulation, deoxycytidine as substrate Homo sapiens

General Stability

General Stability Organism
bovine serum albumin stabilizes Homo sapiens
dTTP, ATP or deoxycytidine stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information deoxycytidine phosphorylating ability shows different inhibition pattern than deoxyadenosine activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxycytidine Homo sapiens key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs ADP + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell from spleen Homo sapiens
-
spleen
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, t1/2: 7 days with and t1/2: 72 h without bovine serum albumin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
Homo sapiens ADP + 5'-dCMP
-
?
ATP + deoxycytidine key anabolic enzyme for activation of purine or pyrimidine deoxynucleosides as well as cytidine arabinoside and other anti-tumor drugs Homo sapiens ADP + ?
-
?
additional information multisubstrate enzyme, that also phosphorylates purine deoxyribonucleotides Homo sapiens ?
-
?
additional information enzyme exists in different conformational states with different substrate kinetic properties Homo sapiens ?
-
?
NTP + deoxyadenosine
-
Homo sapiens NDP + dAMP
-
?
NTP + deoxycytidine
-
Homo sapiens NDP + dCMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 55 about half-maximal activity at 37°C and 55°C Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
temperature stability of deoxycytidine kinase activity differs appreciably from deoxyadenosine kinase activity Homo sapiens
37
-
at least 6 h stable Homo sapiens
54
-
inactivation Homo sapiens