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2.7.1.59: N-acetylglucosamine kinase

This is an abbreviated version!
For detailed information about N-acetylglucosamine kinase, go to the full flat file.

Word Map on EC 2.7.1.59

Reaction

ATP
+
N-acetyl-D-glucosamine
=
ADP
+
N-acetyl-D-glucosamine 6-phosphate

Synonyms

2-acetylamino-2-deoxy-D-glucose kinase, acetylaminodeoxyglucokinase, acetylglucosamine kinase(phosphorylating), ATP:2-acetylamino-2-deoxy-D-glucose 6-phosphotransferase, CaNag5p, GlcNAc kinase, GlcNAc-kinase, GNK, Hxk1, MurK, N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, N-acetylmuramic acid/N-acetylglucosamine kinase, NAG5, NAGA kinase, NagK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.59 N-acetylglucosamine kinase

Purification

Purification on EC 2.7.1.59 - N-acetylglucosamine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from E. coli
from liver and kidney
-
fusion protein from Escherichia coli
glutathione Sepharose column chromatography, and gel filtration
-
glutathione Sepharose column chromatography, MonoQ HR 5/5 column chromatography, and Superdex75 gel filtration
native enzyme 842fold from K12 strain MG1655 in a multistep procedure, recombinant enzyme from strain XL1-Blue
-
Ni-NTA column chromatography
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinnat TAP- and His-tagged HXK1 by tandem affinity purification using anti-FLAG and Ni-nitrilotriacetic acid agarose
-
Talon metal affinity resin column chromatography, and gel filtration
-