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Literature summary for 2.7.1.59 extracted from

  • Flores, C.L.; Gancedo, C.
    The gene YALI0E20207g from Yarrowia lipolytica encodes an N-acetylglucosamine kinase implicated in the regulated expression of the genes from the N-acetylglucosamine assimilatory pathway (2015), PLoS ONE, 10, e0122135.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.59

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bl21(DE3) cells and in a Saccharomyces cerevisiae hxk1 hxk2 glk1 triple mutant Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 N-acetyl-D-glucosamine at pH 7.5 and 30°C Yarrowia lipolytica
1
-
 ATP at pH 7.5 and 30°C Yarrowia lipolytica
67
-
 D-mannose at pH 7.5 and 30°C Yarrowia lipolytica
135
-
 D-glucose at pH 7.5 and 30°C Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Yarrowia lipolytica 100% activity ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine Yarrowia lipolytica CJM936 100% activity ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-
Yarrowia lipolytica CJM936
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography, and gel filtration Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose 14% activity compared to N-acetyl-D-glucosamine Yarrowia lipolytica ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose 14% activity compared to N-acetyl-D-glucosamine Yarrowia lipolytica CJM936 ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose 5% activity compared to N-acetyl-D-glucosamine Yarrowia lipolytica ADP + D-mannose 6-phosphate
-
 ?
ATP + D-mannose 5% activity compared to N-acetyl-D-glucosamine Yarrowia lipolytica CJM936 ADP + D-mannose 6-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine 100% activity Yarrowia lipolytica ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
ATP + N-acetyl-D-glucosamine 100% activity Yarrowia lipolytica CJM936 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
additional information no activity with D-fructose Yarrowia lipolytica ?
-
 ?
additional information no activity with D-fructose Yarrowia lipolytica CJM936 ?
-
 ?

Synonyms

Synonyms Comment Organism
N-acetylglucosamine kinase
-
 Yarrowia lipolytica
NAG5
-
 Yarrowia lipolytica
NAGA kinase
-
 Yarrowia lipolytica