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Literature summary for 2.7.1.59 extracted from

  • Datta, A.
    N-Acetyl-D-glucosamine kinase from hog spleen (1975), Methods Enzymol., 42c, 58-62.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2- mercaptoethanol
-
Sus scrofa
cysteine reverses inhibition with p-chloroxymercuribenzoate Sus scrofa

General Stability

General Stability Organism
unstable to freezing and thawing, heat and low pH Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
Sus scrofa
diphosphate
-
Sus scrofa
N-acetyl-D-glucosamine-6-phosphate non-competitive Sus scrofa
p-chloromercuribenzoate reversible with cysteine or 2-mercaptoethanol Sus scrofa
phosphate
-
Sus scrofa
UDP-N-acetylglucosamine
-
Sus scrofa
UTP
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
N-acetyl-D-glucosamine 37°C, pH 9.0 Sus scrofa
1.8
-
ATP 37°C, pH 9.0 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Sus scrofa
Mg2+ required Sus scrofa
Mn2+ can partially replace Mg2+ in activation Sus scrofa
additional information no activity with Ca2+, Ba2+ Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Sus scrofa involved in UDP-N-acetylglucosamine formation ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
purified enzyme, 37°C, pH 9.5 Sus scrofa

Storage Stability

Storage Stability Organism
4°C, 0.02 M potassium phosphate buffer, pH 7.6, 0.001 M EDTA, 0.01 M 2-mercaptoethanol, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-glucosamine
-
Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine involved in UDP-N-acetylglucosamine formation Sus scrofa ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
GTP + N-acetyl-D-glucosamine 30% of the activity with ATP Sus scrofa GDP + N-acetyl-D-glucosamine 6-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
50% loss of activity within 2 min Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 9.4
-
Sus scrofa