Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2- mercaptoethanol | - |
Sus scrofa | |
cysteine | reverses inhibition with p-chloroxymercuribenzoate | Sus scrofa |
General Stability | Organism |
---|---|
unstable to freezing and thawing, heat and low pH | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Sus scrofa | |
diphosphate | - |
Sus scrofa | |
N-acetyl-D-glucosamine-6-phosphate | non-competitive | Sus scrofa | |
p-chloromercuribenzoate | reversible with cysteine or 2-mercaptoethanol | Sus scrofa | |
phosphate | - |
Sus scrofa | |
UDP-N-acetylglucosamine | - |
Sus scrofa | |
UTP | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
N-acetyl-D-glucosamine | 37°C, pH 9.0 | Sus scrofa | |
1.8 | - |
ATP | 37°C, pH 9.0 | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Sus scrofa | |
Mg2+ | required | Sus scrofa | |
Mn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
additional information | no activity with Ca2+, Ba2+ | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Sus scrofa | involved in UDP-N-acetylglucosamine formation | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
purified enzyme, 37°C, pH 9.5 | Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, 0.02 M potassium phosphate buffer, pH 7.6, 0.001 M EDTA, 0.01 M 2-mercaptoethanol, stable for at least a week | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | - |
Sus scrofa | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | involved in UDP-N-acetylglucosamine formation | Sus scrofa | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
GTP + N-acetyl-D-glucosamine | 30% of the activity with ATP | Sus scrofa | GDP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
50% loss of activity within 2 min | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 9.4 | - |
Sus scrofa |